First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants
Abstract Background Rickettsial infections are often neglected and poorly recognized by physicians in many tropical and subtropical regions. Despite a number of recent reports describing rickettsial diseases in new locations and the discovery of new rickettsiae, medical science and research have lar...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70014 |
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| author | Nagwa Eisawi Jabbar Ahmed Mohammed A. Bakheit Dina A. Hassan Mohammed O. Hussien Abdel Rahim M. El Hussein |
| author_facet | Nagwa Eisawi Jabbar Ahmed Mohammed A. Bakheit Dina A. Hassan Mohammed O. Hussien Abdel Rahim M. El Hussein |
| author_sort | Nagwa Eisawi |
| collection | DOAJ |
| description | Abstract Background Rickettsial infections are often neglected and poorly recognized by physicians in many tropical and subtropical regions. Despite a number of recent reports describing rickettsial diseases in new locations and the discovery of new rickettsiae, medical science and research have largely neglected the diagnosis and antimicrobial treatment of rickettsial infections in subtropical and tropical areas; thus, much remains to be discovered. This study aimed to detect and characterize spotted fever group (SFG) rickettsiae in ixodid ticks infesting domestic ruminants in Khartoum State. Methods Polymerase chain reaction targeting both genes that encode for citrate synthase (gltA) and outer membrane protein (ompA) was performed for the presence of SFG rickettsia followed by sequence and phylogenetic analysis. Results Of the 202 ticks examined for the presence of SFG rickettsia, gltA gene was detected in 4 samples (2%). Furthermore, gltA‐positive samples were used to amplify the ompA gene, in which only two samples yielded positive results. Sequence and phylogenetic analysis of the positive samples revealed four different species of SFG rickettsiae: Rickettsia aeschlimannii, Rickettsia rhipicephali, Rickettsia massiliae and Rickettsia raoultii. Conclusions These results indicated the presence of SFG rickettsia in Sudanese ticks. This also indicates that humans have an opportunity to acquire these infections. It is important to keep in mind the need for careful consideration of rickettsial infections in individuals with a fever of unknown origin. |
| format | Article |
| id | doaj-art-9ec0266186a1422ea96fd353014e3c92 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-9ec0266186a1422ea96fd353014e3c922025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70014First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminantsNagwa Eisawi0Jabbar Ahmed1Mohammed A. Bakheit2Dina A. Hassan3Mohammed O. Hussien4Abdel Rahim M. El Hussein5Central Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanDivision of Veterinary Infection Biology and Immunology Research Center Borstel Borstel GermanyFaculty of Veterinary Medicine University of Khartoum Khartoum North SudanCentral Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanCentral Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanCentral Laboratory Ministry of Higher Education and Scientific Research Khartoum SudanAbstract Background Rickettsial infections are often neglected and poorly recognized by physicians in many tropical and subtropical regions. Despite a number of recent reports describing rickettsial diseases in new locations and the discovery of new rickettsiae, medical science and research have largely neglected the diagnosis and antimicrobial treatment of rickettsial infections in subtropical and tropical areas; thus, much remains to be discovered. This study aimed to detect and characterize spotted fever group (SFG) rickettsiae in ixodid ticks infesting domestic ruminants in Khartoum State. Methods Polymerase chain reaction targeting both genes that encode for citrate synthase (gltA) and outer membrane protein (ompA) was performed for the presence of SFG rickettsia followed by sequence and phylogenetic analysis. Results Of the 202 ticks examined for the presence of SFG rickettsia, gltA gene was detected in 4 samples (2%). Furthermore, gltA‐positive samples were used to amplify the ompA gene, in which only two samples yielded positive results. Sequence and phylogenetic analysis of the positive samples revealed four different species of SFG rickettsiae: Rickettsia aeschlimannii, Rickettsia rhipicephali, Rickettsia massiliae and Rickettsia raoultii. Conclusions These results indicated the presence of SFG rickettsia in Sudanese ticks. This also indicates that humans have an opportunity to acquire these infections. It is important to keep in mind the need for careful consideration of rickettsial infections in individuals with a fever of unknown origin.https://doi.org/10.1002/vms3.70014gltAKhartoum StateompASFG rickettsiaeticks |
| spellingShingle | Nagwa Eisawi Jabbar Ahmed Mohammed A. Bakheit Dina A. Hassan Mohammed O. Hussien Abdel Rahim M. El Hussein First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants Veterinary Medicine and Science gltA Khartoum State ompA SFG rickettsiae ticks |
| title | First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants |
| title_full | First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants |
| title_fullStr | First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants |
| title_full_unstemmed | First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants |
| title_short | First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants |
| title_sort | first molecular diagnosis of the human pathogen rickettsia raoultii and other spotted fever group rickettsiae in sudanese ixodid ticks from domestic ruminants |
| topic | gltA Khartoum State ompA SFG rickettsiae ticks |
| url | https://doi.org/10.1002/vms3.70014 |
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