Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
This study established an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to study the pharmacokinetics of four antiepileptic drugs, lamotrigine, oxcarbazepine, lacosamide, and topiramate, in rats after oral administration. The gradient elution was performed on a...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/1838645 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551266795913216 |
|---|---|
| author | Erjie Qiu Lu Yu Qishun Liang Congcong Wen |
| author_facet | Erjie Qiu Lu Yu Qishun Liang Congcong Wen |
| author_sort | Erjie Qiu |
| collection | DOAJ |
| description | This study established an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to study the pharmacokinetics of four antiepileptic drugs, lamotrigine, oxcarbazepine, lacosamide, and topiramate, in rats after oral administration. The gradient elution was performed on a UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) column with acetonitrile-0.1% formic acid as the mobile phase at a flow rate of 0.4 mL/min. Protein precipitation by acetonitrile was adopted for plasma sample pretreatment. Electrospray- (ESI-) positive/negative ion switching and multiple reaction monitoring (MRM) modes were adopted for ion quantitative determination of antiepileptic drugs. UPLC-MS/MS detection and Drug and Statistics (DAS) software fitting were performed to blood samples collected from rats after oral administration of lamotrigine, oxcarbazepine, lacosamide, and topiramate (5 mg/kg). All drugs examined showed linearity within 5–5000 ng/ml (R2 > 0.9987), the intraday accuracy was within 92%–108%, and the interday accuracy was within 93%–109%. The relative standard deviations (RSD) of intraday and interday were less than 15%. The matrix effect was within 91%–105%, and the recovery was better than 88%. The established UPLC-MS/MS method was successfully applied to the pharmacokinetic study of lamotrigine, oxcarbazepine, lacosamide, and topiramate in rats. |
| format | Article |
| id | doaj-art-9ebc9a0437df4507a8dce47f0cbff200 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-9ebc9a0437df4507a8dce47f0cbff2002025-02-03T06:01:51ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/1838645Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass SpectrometryErjie Qiu0Lu Yu1Qishun Liang2Congcong Wen3Department of Clinical PharmacyDepartment of Clinical PharmacyLaboratory Animal CentreLaboratory Animal CentreThis study established an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to study the pharmacokinetics of four antiepileptic drugs, lamotrigine, oxcarbazepine, lacosamide, and topiramate, in rats after oral administration. The gradient elution was performed on a UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) column with acetonitrile-0.1% formic acid as the mobile phase at a flow rate of 0.4 mL/min. Protein precipitation by acetonitrile was adopted for plasma sample pretreatment. Electrospray- (ESI-) positive/negative ion switching and multiple reaction monitoring (MRM) modes were adopted for ion quantitative determination of antiepileptic drugs. UPLC-MS/MS detection and Drug and Statistics (DAS) software fitting were performed to blood samples collected from rats after oral administration of lamotrigine, oxcarbazepine, lacosamide, and topiramate (5 mg/kg). All drugs examined showed linearity within 5–5000 ng/ml (R2 > 0.9987), the intraday accuracy was within 92%–108%, and the interday accuracy was within 93%–109%. The relative standard deviations (RSD) of intraday and interday were less than 15%. The matrix effect was within 91%–105%, and the recovery was better than 88%. The established UPLC-MS/MS method was successfully applied to the pharmacokinetic study of lamotrigine, oxcarbazepine, lacosamide, and topiramate in rats.http://dx.doi.org/10.1155/2022/1838645 |
| spellingShingle | Erjie Qiu Lu Yu Qishun Liang Congcong Wen Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry International Journal of Analytical Chemistry |
| title | Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | simultaneous determination of lamotrigine oxcarbazepine lacosamide and topiramate in rat plasma by ultra performance liquid chromatography tandem mass spectrometry |
| url | http://dx.doi.org/10.1155/2022/1838645 |
| work_keys_str_mv | AT erjieqiu simultaneousdeterminationoflamotrigineoxcarbazepinelacosamideandtopiramateinratplasmabyultraperformanceliquidchromatographytandemmassspectrometry AT luyu simultaneousdeterminationoflamotrigineoxcarbazepinelacosamideandtopiramateinratplasmabyultraperformanceliquidchromatographytandemmassspectrometry AT qishunliang simultaneousdeterminationoflamotrigineoxcarbazepinelacosamideandtopiramateinratplasmabyultraperformanceliquidchromatographytandemmassspectrometry AT congcongwen simultaneousdeterminationoflamotrigineoxcarbazepinelacosamideandtopiramateinratplasmabyultraperformanceliquidchromatographytandemmassspectrometry |