Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia
Prophages are bacteriophages that integrate their genomes into the bacterial chromosome. This research aimed to analyze and characterize prophages integrated into 44 Pseudomonas aeruginosa strains isolated from tertiary hospitals in Saudi Arabia. A total of 97 intact prophages were identified among...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1563781/full |
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| author | Ahlam Alsaadi Mohammed Imam Abdulrahman A. Alghamdi Safia S. Aljedani Amal Alsari Haya Aljami Mohammad Bosaeed Mohammad Bosaeed |
| author_facet | Ahlam Alsaadi Mohammed Imam Abdulrahman A. Alghamdi Safia S. Aljedani Amal Alsari Haya Aljami Mohammad Bosaeed Mohammad Bosaeed |
| author_sort | Ahlam Alsaadi |
| collection | DOAJ |
| description | Prophages are bacteriophages that integrate their genomes into the bacterial chromosome. This research aimed to analyze and characterize prophages integrated into 44 Pseudomonas aeruginosa strains isolated from tertiary hospitals in Saudi Arabia. A total of 97 intact prophages were identified among clinical strains, with 16 prophages found present in more than one strain simultaneously. All prophages were found to have lengths ranging from 7.7 kb to 74.1 kb, and their GC content was found to be between 49.91% and 64.9%. Our findings show that prophages are present in the majority of the isolated P. aeruginosa strains (41 out of 44). Additionally, several proteins related to viral defense (toxin/antitoxin modules and proteins against restriction-modification enzymes) were identified, supporting the idea that prophages influence bacterial pathogenesis and anti-phage defenses. |
| format | Article |
| id | doaj-art-9e93e7cdc8df4e80a2ba46da1cbcf7bd |
| institution | OA Journals |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-9e93e7cdc8df4e80a2ba46da1cbcf7bd2025-08-20T02:19:58ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-04-011510.3389/fcimb.2025.15637811563781Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi ArabiaAhlam Alsaadi0Mohammed Imam1Abdulrahman A. Alghamdi2Safia S. Aljedani3Amal Alsari4Haya Aljami5Mohammad Bosaeed6Mohammad Bosaeed7King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaDepartment of Microbiology and Parasitology, Qunfudah College of Medicine, Umm Al-Qura University, Al-Qunfudah, Makkah, Saudi ArabiaKing Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaKing Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaKing Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaKing Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaKing Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaDepartment of Medicine, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, Saudi ArabiaProphages are bacteriophages that integrate their genomes into the bacterial chromosome. This research aimed to analyze and characterize prophages integrated into 44 Pseudomonas aeruginosa strains isolated from tertiary hospitals in Saudi Arabia. A total of 97 intact prophages were identified among clinical strains, with 16 prophages found present in more than one strain simultaneously. All prophages were found to have lengths ranging from 7.7 kb to 74.1 kb, and their GC content was found to be between 49.91% and 64.9%. Our findings show that prophages are present in the majority of the isolated P. aeruginosa strains (41 out of 44). Additionally, several proteins related to viral defense (toxin/antitoxin modules and proteins against restriction-modification enzymes) were identified, supporting the idea that prophages influence bacterial pathogenesis and anti-phage defenses.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1563781/fullPseudomonas aeruginosaprophagesmulti-drug resistancebacteriophagesSaudi Arabia |
| spellingShingle | Ahlam Alsaadi Mohammed Imam Abdulrahman A. Alghamdi Safia S. Aljedani Amal Alsari Haya Aljami Mohammad Bosaeed Mohammad Bosaeed Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia Frontiers in Cellular and Infection Microbiology Pseudomonas aeruginosa prophages multi-drug resistance bacteriophages Saudi Arabia |
| title | Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia |
| title_full | Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia |
| title_fullStr | Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia |
| title_full_unstemmed | Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia |
| title_short | Genomic analysis of prophages in 44 clinical strains of Pseudomonas aeruginosa isolated in Saudi Arabia |
| title_sort | genomic analysis of prophages in 44 clinical strains of pseudomonas aeruginosa isolated in saudi arabia |
| topic | Pseudomonas aeruginosa prophages multi-drug resistance bacteriophages Saudi Arabia |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1563781/full |
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