Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study
Abstract Background Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers in the world. The major etiological factors are considered to be tobacco and alcohol. However, the etiological factors for non-habit associated oral squamous cell carcinoma (NHOSCC) remains an enigma. S...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12903-024-05406-z |
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| author | Mudiyayirakkani Muthusamy Pratibha Ramani Paramasivam Arumugam Parthiban Rudrapathy Boopathi Kangusamy Vishnu Priya Veeraraghavan Selvaraj Jayaraman Balachander Kannan Anitha Pandi |
| author_facet | Mudiyayirakkani Muthusamy Pratibha Ramani Paramasivam Arumugam Parthiban Rudrapathy Boopathi Kangusamy Vishnu Priya Veeraraghavan Selvaraj Jayaraman Balachander Kannan Anitha Pandi |
| author_sort | Mudiyayirakkani Muthusamy |
| collection | DOAJ |
| description | Abstract Background Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers in the world. The major etiological factors are considered to be tobacco and alcohol. However, the etiological factors for non-habit associated oral squamous cell carcinoma (NHOSCC) remains an enigma. So we focused in assessing various etiological factors like genetic factor, microbial factor, dental factor and the biochemical factor of non-habit associated oral squamous cell carcinoma. The aim was to assess Harvey Rat Sarcoma Virus gene (HRAS) mutation, total bacterial count, Herpes Simplex Virus-1 (HSV-1), regressive changes of teeth, total antioxidant capacity and its association with NHOSCC. Materials and methods A total of 564 (n = 564) patients with OSCC were included in the study. Out of 564 patients, 282 patients had NHOSCC and 282 patients had habit associated oral squamous cell carcinoma (HOSCC). The isolated DNA from the tissue was subjected to Sanger’s sequencing analysis for mutation analysis of the HRAS gene. The isolated serum was subjected to HSV-1 ELISA analysis and TAC ELISA analysis. The dental cast used to analyze the presence of sharp teeth/ any other form of regressive changes of teeth. Results Firstly, we found 3 novel pathogenic mutations c.16C > A/p.L6M (missense mutation), c.359 T > C/p.L120P (point mutation), c.382C > T/p.R128W (missense mutation) of HRAS gene in NHOSCC samples by genetic analysis. No significant difference was noted in the total bacterial count between the non-habit associated and habit associated oral squamous cell carcinoma (HOSCC). The binary logistic regression showed patients with HSV1 infection have 2.667 odds (2.667 OR, CI, 1.589- 4.484) of getting NHOSCC and it was found to be statistically significant (p < 0.001).The dental analysis revealed that patients with regressive changes have 4.432 odds (4.432 OR, CI, 2.807- 6.998) of getting NHOSCC and it was found to be statistically significant (p < 0.001). The biochemical analysis revealed patients with lower total antioxidant capacity have 0.671 odds (0.671 OR, CI, 0.621–0.725) of getting NHOSCC and was found to be statistically significant (p < 0.001). Our results suggest that the frequency of HRAS mutation in NHOSCC is high. HSV1, oxidative stress and regressive changes of teeth are associated with NHOSCC. Conclusion Our results suggest that the frequency of HRAS mutation in NHOSCC is high. HSV1, oxidative stress and regressive changes of teeth are associated with NHOSCC. |
| format | Article |
| id | doaj-art-9e7b5b5215f6416f95971dbd0efa7179 |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-01-01 |
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| series | BMC Oral Health |
| spelling | doaj-art-9e7b5b5215f6416f95971dbd0efa71792025-01-19T12:41:01ZengBMCBMC Oral Health1472-68312025-01-0125111810.1186/s12903-024-05406-zAssessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control studyMudiyayirakkani Muthusamy0Pratibha Ramani1Paramasivam Arumugam2Parthiban Rudrapathy3Boopathi Kangusamy4Vishnu Priya Veeraraghavan5Selvaraj Jayaraman6Balachander Kannan7Anitha Pandi8Department of Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesDepartment of Oral and Maxillofacial Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesSaveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesMicrobiology Division, Department of CLSTR, Malabar Cancer Centre, Post Graduate Institute of Oncological Sciences and ResearchICMR-National Institute of EpidemiologyCentre of Molecular Medicine and Diagnostics, Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesCentre of Molecular Medicine and Diagnostics, Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesMolecular Biology Laboratory, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesClinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical SciencesAbstract Background Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers in the world. The major etiological factors are considered to be tobacco and alcohol. However, the etiological factors for non-habit associated oral squamous cell carcinoma (NHOSCC) remains an enigma. So we focused in assessing various etiological factors like genetic factor, microbial factor, dental factor and the biochemical factor of non-habit associated oral squamous cell carcinoma. The aim was to assess Harvey Rat Sarcoma Virus gene (HRAS) mutation, total bacterial count, Herpes Simplex Virus-1 (HSV-1), regressive changes of teeth, total antioxidant capacity and its association with NHOSCC. Materials and methods A total of 564 (n = 564) patients with OSCC were included in the study. Out of 564 patients, 282 patients had NHOSCC and 282 patients had habit associated oral squamous cell carcinoma (HOSCC). The isolated DNA from the tissue was subjected to Sanger’s sequencing analysis for mutation analysis of the HRAS gene. The isolated serum was subjected to HSV-1 ELISA analysis and TAC ELISA analysis. The dental cast used to analyze the presence of sharp teeth/ any other form of regressive changes of teeth. Results Firstly, we found 3 novel pathogenic mutations c.16C > A/p.L6M (missense mutation), c.359 T > C/p.L120P (point mutation), c.382C > T/p.R128W (missense mutation) of HRAS gene in NHOSCC samples by genetic analysis. No significant difference was noted in the total bacterial count between the non-habit associated and habit associated oral squamous cell carcinoma (HOSCC). The binary logistic regression showed patients with HSV1 infection have 2.667 odds (2.667 OR, CI, 1.589- 4.484) of getting NHOSCC and it was found to be statistically significant (p < 0.001).The dental analysis revealed that patients with regressive changes have 4.432 odds (4.432 OR, CI, 2.807- 6.998) of getting NHOSCC and it was found to be statistically significant (p < 0.001). The biochemical analysis revealed patients with lower total antioxidant capacity have 0.671 odds (0.671 OR, CI, 0.621–0.725) of getting NHOSCC and was found to be statistically significant (p < 0.001). Our results suggest that the frequency of HRAS mutation in NHOSCC is high. HSV1, oxidative stress and regressive changes of teeth are associated with NHOSCC. Conclusion Our results suggest that the frequency of HRAS mutation in NHOSCC is high. HSV1, oxidative stress and regressive changes of teeth are associated with NHOSCC.https://doi.org/10.1186/s12903-024-05406-zNon habit associated oral squamous cell carcinomaHRAS mutationHSV1Regressive changes of toothOxidative stress |
| spellingShingle | Mudiyayirakkani Muthusamy Pratibha Ramani Paramasivam Arumugam Parthiban Rudrapathy Boopathi Kangusamy Vishnu Priya Veeraraghavan Selvaraj Jayaraman Balachander Kannan Anitha Pandi Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study BMC Oral Health Non habit associated oral squamous cell carcinoma HRAS mutation HSV1 Regressive changes of tooth Oxidative stress |
| title | Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study |
| title_full | Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study |
| title_fullStr | Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study |
| title_full_unstemmed | Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study |
| title_short | Assessment of various etiological factors for oral squamous cell carcinoma in non-habit patients- a cross sectional case control study |
| title_sort | assessment of various etiological factors for oral squamous cell carcinoma in non habit patients a cross sectional case control study |
| topic | Non habit associated oral squamous cell carcinoma HRAS mutation HSV1 Regressive changes of tooth Oxidative stress |
| url | https://doi.org/10.1186/s12903-024-05406-z |
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