Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis
This study aimed to produce, characterize, and optimize glucose syrup from Colocasia esculenta starch through enzymatic hydrolysis, an area that remains insufficiently explored and studied in the existing literature. The process involved starch extraction from Colocasia esculenta and three sequentia...
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Elsevier
2025-07-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625003807 |
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| author | Bethelhem Teshome Mikiyas Abewaa Jemal Abdu Magesh Rangaraju Ashagrie Mengistu Solomon Tibebu Tigabu Haddis |
| author_facet | Bethelhem Teshome Mikiyas Abewaa Jemal Abdu Magesh Rangaraju Ashagrie Mengistu Solomon Tibebu Tigabu Haddis |
| author_sort | Bethelhem Teshome |
| collection | DOAJ |
| description | This study aimed to produce, characterize, and optimize glucose syrup from Colocasia esculenta starch through enzymatic hydrolysis, an area that remains insufficiently explored and studied in the existing literature. The process involved starch extraction from Colocasia esculenta and three sequential steps were followed for the extraction of glucose syrup. These stages include gelatinization, liquefaction, and saccharification. The physicochemical properties of Colocasia esculenta-derived starch were analyzed, revealing a moisture content of 12 %, water-holding capacity of 70 %, ash content of 0.66 %, particle size distribution of 7 μm, amylose content of 23.57 %, bulk density of 0.87 g/cm3, and a pH of 7.2. The study investigated the effects of pH, mixing time, and enzyme concentration on glucose syrup yield. The experiments were conducted at a constant temperature of 62.5 °C, with enzyme concentrations of 0.3, 0.35, and 0.4 mg/L, hydrolysis times of 240, 480, and 720 min, and pH levels of 4.5, 4.75, and 5. The results showed that optimal conditions of pH 4.75, enzyme concentration of 0.40 mg/L, and hydrolysis time of 720 min yielded the highest reducing sugar content (46 %), degree Brix (44.2 %), and maximum glucose yield (76 %). Overall, this study demonstrated that locally available Colocasia esculenta is a viable source for producing glucose syrup with desirable characteristics. |
| format | Article |
| id | doaj-art-9e5f8a5e162c42e6a48bcccb06160f0d |
| institution | DOAJ |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-9e5f8a5e162c42e6a48bcccb06160f0d2025-08-20T03:23:34ZengElsevierResults in Chemistry2211-71562025-07-011610239710.1016/j.rechem.2025.102397Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysisBethelhem Teshome0Mikiyas Abewaa1Jemal Abdu2Magesh Rangaraju3Ashagrie Mengistu4Solomon Tibebu5Tigabu Haddis6Department of Chemical Engineering, College of Engineering and Technology, Wachemo University, P.O. Box. 667, Hossana, EthiopiaDepartment of Chemical Engineering, College of Engineering and Technology, Wachemo University, P.O. Box. 667, Hossana, Ethiopia; Corresponding author.Department of Chemical Engineering, College of Engineering and Technology, Wachemo University, P.O. Box. 667, Hossana, EthiopiaDepartment of Chemical Engineering, Adhiparasakthi Engineering College, P.O. BOX. 603319, Melmaruvathur, IndiaThe Federal Democratic Republic of Ethiopia, Manufacturing Industry Development Institute, Addis Ababa, EthiopiaDepartment of Environmental Engineering, College of Engineering, Addis Ababa Science and Technology University, Addis Ababa, EthiopiaDepartment of Chemical Engineering, Faculty of Engineering, Ariel University, P.O.Box. 407000, Ariel, IsraelThis study aimed to produce, characterize, and optimize glucose syrup from Colocasia esculenta starch through enzymatic hydrolysis, an area that remains insufficiently explored and studied in the existing literature. The process involved starch extraction from Colocasia esculenta and three sequential steps were followed for the extraction of glucose syrup. These stages include gelatinization, liquefaction, and saccharification. The physicochemical properties of Colocasia esculenta-derived starch were analyzed, revealing a moisture content of 12 %, water-holding capacity of 70 %, ash content of 0.66 %, particle size distribution of 7 μm, amylose content of 23.57 %, bulk density of 0.87 g/cm3, and a pH of 7.2. The study investigated the effects of pH, mixing time, and enzyme concentration on glucose syrup yield. The experiments were conducted at a constant temperature of 62.5 °C, with enzyme concentrations of 0.3, 0.35, and 0.4 mg/L, hydrolysis times of 240, 480, and 720 min, and pH levels of 4.5, 4.75, and 5. The results showed that optimal conditions of pH 4.75, enzyme concentration of 0.40 mg/L, and hydrolysis time of 720 min yielded the highest reducing sugar content (46 %), degree Brix (44.2 %), and maximum glucose yield (76 %). Overall, this study demonstrated that locally available Colocasia esculenta is a viable source for producing glucose syrup with desirable characteristics.http://www.sciencedirect.com/science/article/pii/S2211715625003807Colocasia esculentaEnzyme hydrolysisExtractionGlucose syrupOptimizationStarch |
| spellingShingle | Bethelhem Teshome Mikiyas Abewaa Jemal Abdu Magesh Rangaraju Ashagrie Mengistu Solomon Tibebu Tigabu Haddis Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis Results in Chemistry Colocasia esculenta Enzyme hydrolysis Extraction Glucose syrup Optimization Starch |
| title | Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis |
| title_full | Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis |
| title_fullStr | Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis |
| title_full_unstemmed | Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis |
| title_short | Optimization of glucose syrup extraction from Colocasia esculenta starch using enzymatic hydrolysis |
| title_sort | optimization of glucose syrup extraction from colocasia esculenta starch using enzymatic hydrolysis |
| topic | Colocasia esculenta Enzyme hydrolysis Extraction Glucose syrup Optimization Starch |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625003807 |
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