Protocol for Agrobacterium-mediated transformation of tomato and flow cytometry-based ploidy confirmation
Summary: Here, we present an Agrobacterium-mediated transformation protocol for tomato, Solanum lycopersicum cv. Micro-Tom, which incorporates flow cytometric ploidy analysis, a critical yet often overlooked step. We detail steps for cotyledon explants, inoculating with Agrobacterium, and co-cultiva...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672500365X |
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