A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye

<b>Background/Objectives</b>: Cerebral folate transporter deficiency is characterized by pauses and regression in general development stages, with ataxia, choreoathetoid movements, and myoclonic epilepsy generally resistant to treatment. The aim of this study was to comprehensively evalu...

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Main Authors: Abdurrahman Akgun, Ibrahim Tas
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Diagnostics
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Online Access:https://www.mdpi.com/2075-4418/15/7/892
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author Abdurrahman Akgun
Ibrahim Tas
author_facet Abdurrahman Akgun
Ibrahim Tas
author_sort Abdurrahman Akgun
collection DOAJ
description <b>Background/Objectives</b>: Cerebral folate transporter deficiency is characterized by pauses and regression in general development stages, with ataxia, choreoathetoid movements, and myoclonic epilepsy generally resistant to treatment. The aim of this study was to comprehensively evaluate cases followed up in two centres in Türkiye for a diagnosis of folate receptor-α deficiency. <b>Methods</b>: The study included nine cases from six different families. <b>Results</b>: The patients comprised 22.2% males and there was parental consanguinity in 88.9% of cases. The mean age at which complaints were first noticed was 3.7 years, and the age of definitive diagnosis was 10.4 years. The most frequently seen first complaints were febrile convulsions and attention deficit-hyperactivity-learning difficulties. The diagnosis most commonly made before the definitive diagnosis was epilepsy, and the first seizure occurred at a mean of 5.2 years. On cranial imaging, white matter involvement, cerebellar atrophy and cerebral atrophy were determined most often. Definitive diagnosis was established solely through clinical findings and genetic analysis. Three different variants in the <i>FOLR1</i> gene were determined. Treatment with folinic acid at a dose of 5.2 mg/kg/day of PO was started at the age of 9.8 years on average, and intravenous folinate was started at different doses. <b>Conclusions</b>: This study stands out as one of the largest case series in the literature and identifies a previously unreported novel variant. Our study suggests that FOLR1-related CFD should be considered in cases with febrile convulsions, developmental delay, ataxia, autism spectrum disorder, acquired microcephaly, and MRI findings of white matter involvement and cerebellar atrophy. Due to an asymptomatic early period, CFD diagnosis may be delayed, and treatment after symptom onset may be less effective. Incorporating <i>FOLR1</i> gene analysis into newborn screening programmes could facilitate early diagnosis and treatment. It is thought that the application of vagus nerve stimulation, in addition to folinic acid and anticonvulsant drug treatment, could be effective in seizure control.
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spelling doaj-art-9e51cc3f25c2416e9a9b00bbb8e1eda62025-08-20T02:09:11ZengMDPI AGDiagnostics2075-44182025-04-0115789210.3390/diagnostics15070892A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from TürkiyeAbdurrahman Akgun0Ibrahim Tas1Division of Metabolism, Department of Pediatrics, Firat University School of Medicine, 23200 Elazig, TürkiyeClinic of Pediatric Metabolic Diseases, Umraniye Training and Research Hospital, University of Health Sciences, 34766 Istanbul, Türkiye<b>Background/Objectives</b>: Cerebral folate transporter deficiency is characterized by pauses and regression in general development stages, with ataxia, choreoathetoid movements, and myoclonic epilepsy generally resistant to treatment. The aim of this study was to comprehensively evaluate cases followed up in two centres in Türkiye for a diagnosis of folate receptor-α deficiency. <b>Methods</b>: The study included nine cases from six different families. <b>Results</b>: The patients comprised 22.2% males and there was parental consanguinity in 88.9% of cases. The mean age at which complaints were first noticed was 3.7 years, and the age of definitive diagnosis was 10.4 years. The most frequently seen first complaints were febrile convulsions and attention deficit-hyperactivity-learning difficulties. The diagnosis most commonly made before the definitive diagnosis was epilepsy, and the first seizure occurred at a mean of 5.2 years. On cranial imaging, white matter involvement, cerebellar atrophy and cerebral atrophy were determined most often. Definitive diagnosis was established solely through clinical findings and genetic analysis. Three different variants in the <i>FOLR1</i> gene were determined. Treatment with folinic acid at a dose of 5.2 mg/kg/day of PO was started at the age of 9.8 years on average, and intravenous folinate was started at different doses. <b>Conclusions</b>: This study stands out as one of the largest case series in the literature and identifies a previously unreported novel variant. Our study suggests that FOLR1-related CFD should be considered in cases with febrile convulsions, developmental delay, ataxia, autism spectrum disorder, acquired microcephaly, and MRI findings of white matter involvement and cerebellar atrophy. Due to an asymptomatic early period, CFD diagnosis may be delayed, and treatment after symptom onset may be less effective. Incorporating <i>FOLR1</i> gene analysis into newborn screening programmes could facilitate early diagnosis and treatment. It is thought that the application of vagus nerve stimulation, in addition to folinic acid and anticonvulsant drug treatment, could be effective in seizure control.https://www.mdpi.com/2075-4418/15/7/892cerebral folate transporter deficiencyFOLR15-methyltetrahydrofolatefolic acidfolinic acid
spellingShingle Abdurrahman Akgun
Ibrahim Tas
A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye
Diagnostics
cerebral folate transporter deficiency
FOLR1
5-methyltetrahydrofolate
folic acid
folinic acid
title A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye
title_full A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye
title_fullStr A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye
title_full_unstemmed A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye
title_short A Clinical and Genetic Evaluation of Cases with Folate Receptor α Gene Mutation: A Case Series from Türkiye
title_sort clinical and genetic evaluation of cases with folate receptor α gene mutation a case series from turkiye
topic cerebral folate transporter deficiency
FOLR1
5-methyltetrahydrofolate
folic acid
folinic acid
url https://www.mdpi.com/2075-4418/15/7/892
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