Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes
The present study was conducted to analyze the correlation between the milk fat content of Binglangjiang buffaloes and their microbial and host metabolites. The 10 buffaloes with the highest milk fat content (HF, 5.60 ± 0.61%) and the 10 with the lowest milk fat content (LF, 1.49 ± 0.13%) were selec...
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MDPI AG
2025-01-01
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| author | Ye Yu Runqi Fu Chunjia Jin Lin Han Huan Gao Binlong Fu Min Qi Qian Li Jing Leng |
| author_facet | Ye Yu Runqi Fu Chunjia Jin Lin Han Huan Gao Binlong Fu Min Qi Qian Li Jing Leng |
| author_sort | Ye Yu |
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| description | The present study was conducted to analyze the correlation between the milk fat content of Binglangjiang buffaloes and their microbial and host metabolites. The 10 buffaloes with the highest milk fat content (HF, 5.60 ± 0.61%) and the 10 with the lowest milk fat content (LF, 1.49 ± 0.13%) were selected. Their rumen fluid and plasma were collected for rumen microbiota and metabolome analysis. The results showed that the rumen bacteria abundance of <i>Synergistota</i>, <i>Quinella</i>, <i>Selenomonas</i>, and <i>Fretibacterium</i> was significantly higher in the HF buffaloes. The abundance of 14 rumen fungi, including <i>Candida</i>, <i>Talaromyces</i>, <i>Cyrenella</i>, and <i>Stilbella</i>, was significantly higher in the HF buffaloes. The analysis of the metabolites in the rumen and plasma showed that several metabolites differed between the HF and LF buffaloes. A total of 68 and 42 differential metabolites were identified in the rumen and plasma, respectively. By clustering these differential metabolites, most of those clustered in the HF group were lipid and lipid-like molecules such as secoeremopetasitolide B, lucidenic acid J LysoPE (0:0/18:2 (9Z, 12Z)), and 5-tetradecenoic acid. Spearman’s rank correlations showed that <i>Quinella</i>, <i>Fretibacterium</i>, <i>Selenomonas</i>, <i>Cyrenella</i>, and <i>Stilbella</i> were significantly positively correlated with the metabolites of the lipids and lipid-like molecules in the rumen and plasma. The results suggest that rumen microbiota such as <i>Quinella</i>, <i>Fretibacterium</i>, <i>Selenomonas</i>, and <i>Cyrenella</i> may regulate milk fat synthesis by influencing the lipid metabolites in the rumen and plasma. In addition, the combined analysis of the rumen microbiota and host metabolites may provide a fundamental understanding of the role of the microbiota and host in regulating milk fat synthesis. |
| format | Article |
| id | doaj-art-9e4c864072f64b4aa201915009bf596c |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Animals |
| spelling | doaj-art-9e4c864072f64b4aa201915009bf596c2025-01-24T13:18:16ZengMDPI AGAnimals2076-26152025-01-0115224810.3390/ani15020248Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in BuffaloesYe Yu0Runqi Fu1Chunjia Jin2Lin Han3Huan Gao4Binlong Fu5Min Qi6Qian Li7Jing Leng8Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaThe present study was conducted to analyze the correlation between the milk fat content of Binglangjiang buffaloes and their microbial and host metabolites. The 10 buffaloes with the highest milk fat content (HF, 5.60 ± 0.61%) and the 10 with the lowest milk fat content (LF, 1.49 ± 0.13%) were selected. Their rumen fluid and plasma were collected for rumen microbiota and metabolome analysis. The results showed that the rumen bacteria abundance of <i>Synergistota</i>, <i>Quinella</i>, <i>Selenomonas</i>, and <i>Fretibacterium</i> was significantly higher in the HF buffaloes. The abundance of 14 rumen fungi, including <i>Candida</i>, <i>Talaromyces</i>, <i>Cyrenella</i>, and <i>Stilbella</i>, was significantly higher in the HF buffaloes. The analysis of the metabolites in the rumen and plasma showed that several metabolites differed between the HF and LF buffaloes. A total of 68 and 42 differential metabolites were identified in the rumen and plasma, respectively. By clustering these differential metabolites, most of those clustered in the HF group were lipid and lipid-like molecules such as secoeremopetasitolide B, lucidenic acid J LysoPE (0:0/18:2 (9Z, 12Z)), and 5-tetradecenoic acid. Spearman’s rank correlations showed that <i>Quinella</i>, <i>Fretibacterium</i>, <i>Selenomonas</i>, <i>Cyrenella</i>, and <i>Stilbella</i> were significantly positively correlated with the metabolites of the lipids and lipid-like molecules in the rumen and plasma. The results suggest that rumen microbiota such as <i>Quinella</i>, <i>Fretibacterium</i>, <i>Selenomonas</i>, and <i>Cyrenella</i> may regulate milk fat synthesis by influencing the lipid metabolites in the rumen and plasma. In addition, the combined analysis of the rumen microbiota and host metabolites may provide a fundamental understanding of the role of the microbiota and host in regulating milk fat synthesis.https://www.mdpi.com/2076-2615/15/2/248rumen microbiomerumen metabolomeplasma metabolomemilk fatBinglangjiang buffaloes |
| spellingShingle | Ye Yu Runqi Fu Chunjia Jin Lin Han Huan Gao Binlong Fu Min Qi Qian Li Jing Leng Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes Animals rumen microbiome rumen metabolome plasma metabolome milk fat Binglangjiang buffaloes |
| title | Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes |
| title_full | Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes |
| title_fullStr | Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes |
| title_full_unstemmed | Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes |
| title_short | Multi-Omics Insights into Rumen Microbiota and Metabolite Interactions Regulating Milk Fat Synthesis in Buffaloes |
| title_sort | multi omics insights into rumen microbiota and metabolite interactions regulating milk fat synthesis in buffaloes |
| topic | rumen microbiome rumen metabolome plasma metabolome milk fat Binglangjiang buffaloes |
| url | https://www.mdpi.com/2076-2615/15/2/248 |
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