Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication
Abstract To build in vitro tissues for therapeutic applications, it is essential to replicate the spatial distribution of cells that occurs during morphogenesis in vivo. However, it remains technically challenging to simultaneously regulate the geometric alignment and aggregation of cells during tis...
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| Format: | Article |
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Nature Portfolio
2025-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-59026-4 |
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| author | Byungjun Kang Eunseon Jeong Seung Yeop Han Jeong Hyun Heo Yunam Lee Suah Choi Yunjung Choi Donyoung Kang Youn-Hoo Hwang Jiin Lee Jung Hwa Seo Jinyoung Kim Inhea Jeong Enji Kim Juyoung Lee Dae-Eun Kim Jang-Ung Park Sung-Rae Cho Yoonhee Jin Seung-Woo Cho Hyungsuk Lee |
| author_facet | Byungjun Kang Eunseon Jeong Seung Yeop Han Jeong Hyun Heo Yunam Lee Suah Choi Yunjung Choi Donyoung Kang Youn-Hoo Hwang Jiin Lee Jung Hwa Seo Jinyoung Kim Inhea Jeong Enji Kim Juyoung Lee Dae-Eun Kim Jang-Ung Park Sung-Rae Cho Yoonhee Jin Seung-Woo Cho Hyungsuk Lee |
| author_sort | Byungjun Kang |
| collection | DOAJ |
| description | Abstract To build in vitro tissues for therapeutic applications, it is essential to replicate the spatial distribution of cells that occurs during morphogenesis in vivo. However, it remains technically challenging to simultaneously regulate the geometric alignment and aggregation of cells during tissue fabrication. Here, we introduce the acoustofluidic bioassembly induced morphogenesis, which is the combination of precise arrangement of cells by the mechanical forces produced by acoustofluidic cues, and the morphological and functional changes of cells in the following in vitro and in vivo cultures. The acoustofluidic bioassembly can be used to create tissues with regulated nano-, micro-, and macro-structures. We demonstrate that the neuromuscular tissue fabricated with the acoustofluidic bioassembly exhibits enhanced contraction dynamics, electrophysiology, and therapeutic efficacy. The potential of the acoustofluidic bioassembly as an in situ application is demonstrated by fabricating artificial tissues at the defect sites of living tissues. The acoustofluidic bioassembly induced morphogenesis can provide a pioneering platform to fabricate tissues for biomedical applications. |
| format | Article |
| id | doaj-art-9e4c638c602e4c99b936f5ac791e5558 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-9e4c638c602e4c99b936f5ac791e55582025-08-20T03:09:19ZengNature PortfolioNature Communications2041-17232025-05-0116111910.1038/s41467-025-59026-4Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabricationByungjun Kang0Eunseon Jeong1Seung Yeop Han2Jeong Hyun Heo3Yunam Lee4Suah Choi5Yunjung Choi6Donyoung Kang7Youn-Hoo Hwang8Jiin Lee9Jung Hwa Seo10Jinyoung Kim11Inhea Jeong12Enji Kim13Juyoung Lee14Dae-Eun Kim15Jang-Ung Park16Sung-Rae Cho17Yoonhee Jin18Seung-Woo Cho19Hyungsuk Lee20School of Mechanical Engineering, Yonsei UniversityDepartment of Biotechnology, Yonsei UniversityDepartment of Biotechnology, Yonsei UniversityDepartment of Physiology, Yonsei University College of MedicineSchool of Mechanical Engineering, Yonsei UniversityDepartment of Biotechnology, Yonsei UniversitySchool of Mechanical Engineering, Yonsei UniversitySchool of Mechanical Engineering, Yonsei UniversitySchool of Mechanical Engineering, Yonsei UniversityDepartment of Biotechnology, Yonsei UniversityDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of MedicineDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of MedicineDepartment of Materials Science and Engineering, Yonsei UniversityDepartment of Materials Science and Engineering, Yonsei UniversitySchool of Mechanical Engineering, Yonsei UniversitySchool of Mechanical Engineering, Yonsei UniversityDepartment of Materials Science and Engineering, Yonsei UniversityDepartment and Research Institute of Rehabilitation Medicine, Yonsei University College of MedicineDepartment of Physiology, Yonsei University College of MedicineDepartment of Biotechnology, Yonsei UniversitySchool of Mechanical Engineering, Yonsei UniversityAbstract To build in vitro tissues for therapeutic applications, it is essential to replicate the spatial distribution of cells that occurs during morphogenesis in vivo. However, it remains technically challenging to simultaneously regulate the geometric alignment and aggregation of cells during tissue fabrication. Here, we introduce the acoustofluidic bioassembly induced morphogenesis, which is the combination of precise arrangement of cells by the mechanical forces produced by acoustofluidic cues, and the morphological and functional changes of cells in the following in vitro and in vivo cultures. The acoustofluidic bioassembly can be used to create tissues with regulated nano-, micro-, and macro-structures. We demonstrate that the neuromuscular tissue fabricated with the acoustofluidic bioassembly exhibits enhanced contraction dynamics, electrophysiology, and therapeutic efficacy. The potential of the acoustofluidic bioassembly as an in situ application is demonstrated by fabricating artificial tissues at the defect sites of living tissues. The acoustofluidic bioassembly induced morphogenesis can provide a pioneering platform to fabricate tissues for biomedical applications.https://doi.org/10.1038/s41467-025-59026-4 |
| spellingShingle | Byungjun Kang Eunseon Jeong Seung Yeop Han Jeong Hyun Heo Yunam Lee Suah Choi Yunjung Choi Donyoung Kang Youn-Hoo Hwang Jiin Lee Jung Hwa Seo Jinyoung Kim Inhea Jeong Enji Kim Juyoung Lee Dae-Eun Kim Jang-Ung Park Sung-Rae Cho Yoonhee Jin Seung-Woo Cho Hyungsuk Lee Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication Nature Communications |
| title | Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication |
| title_full | Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication |
| title_fullStr | Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication |
| title_full_unstemmed | Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication |
| title_short | Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication |
| title_sort | acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication |
| url | https://doi.org/10.1038/s41467-025-59026-4 |
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