In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging
Recently, mesenchymal stem cells (MSCs) are increasingly used as a panacea for multiple types of disease short of effective treatment. Dozens of clinical trials published demonstrated strikingly positive therapeutic effects of MSCs. However, as a specific agent, little research has focused on the dy...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/3970942 |
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| author | Juan Cao Shike Hou Hui Ding Ziquan Liu Meijuan Song Xiaojing Qin Xue Wang Mengyang Yu Zhiguang Sun Jinyang Liu Shuli Sun Peixin Xiao Qi Lv Haojun Fan |
| author_facet | Juan Cao Shike Hou Hui Ding Ziquan Liu Meijuan Song Xiaojing Qin Xue Wang Mengyang Yu Zhiguang Sun Jinyang Liu Shuli Sun Peixin Xiao Qi Lv Haojun Fan |
| author_sort | Juan Cao |
| collection | DOAJ |
| description | Recently, mesenchymal stem cells (MSCs) are increasingly used as a panacea for multiple types of disease short of effective treatment. Dozens of clinical trials published demonstrated strikingly positive therapeutic effects of MSCs. However, as a specific agent, little research has focused on the dynamic distribution of MSCs after in vivo administration. In this study, we track systemically transplanted allogeneic bone marrow mesenchymal stem cells (BMSCs) in normal rats through bioluminescence imaging (BLI) in real time. Ex vivo organ imaging, immunohistochemistry (IHC), and RT-PCR were conducted to verify the histological distribution of BMSCs. Our results showed that BMSCs home to the dorsal skin apart from the lungs and kidneys after tail vein injection and could not be detected 14 days later. Allogeneic BMSCs mainly appeared not at the parenchymatous organs but at the subepidermal connective tissue and adipose tissue in healthy rats. There were no significant MSCs-related adverse effects except for transient decrease in neutrophils. These findings will provide experimental evidences for a better understanding of the biocharacteristics of BMSCs. |
| format | Article |
| id | doaj-art-9e49ea5388074ce9969ef2b59e98bd00 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9e49ea5388074ce9969ef2b59e98bd002025-02-03T05:51:22ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/39709423970942In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence ImagingJuan Cao0Shike Hou1Hui Ding2Ziquan Liu3Meijuan Song4Xiaojing Qin5Xue Wang6Mengyang Yu7Zhiguang Sun8Jinyang Liu9Shuli Sun10Peixin Xiao11Qi Lv12Haojun Fan13Institute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaDepartment of Pathology, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin, ChinaRecently, mesenchymal stem cells (MSCs) are increasingly used as a panacea for multiple types of disease short of effective treatment. Dozens of clinical trials published demonstrated strikingly positive therapeutic effects of MSCs. However, as a specific agent, little research has focused on the dynamic distribution of MSCs after in vivo administration. In this study, we track systemically transplanted allogeneic bone marrow mesenchymal stem cells (BMSCs) in normal rats through bioluminescence imaging (BLI) in real time. Ex vivo organ imaging, immunohistochemistry (IHC), and RT-PCR were conducted to verify the histological distribution of BMSCs. Our results showed that BMSCs home to the dorsal skin apart from the lungs and kidneys after tail vein injection and could not be detected 14 days later. Allogeneic BMSCs mainly appeared not at the parenchymatous organs but at the subepidermal connective tissue and adipose tissue in healthy rats. There were no significant MSCs-related adverse effects except for transient decrease in neutrophils. These findings will provide experimental evidences for a better understanding of the biocharacteristics of BMSCs.http://dx.doi.org/10.1155/2016/3970942 |
| spellingShingle | Juan Cao Shike Hou Hui Ding Ziquan Liu Meijuan Song Xiaojing Qin Xue Wang Mengyang Yu Zhiguang Sun Jinyang Liu Shuli Sun Peixin Xiao Qi Lv Haojun Fan In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging Stem Cells International |
| title | In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging |
| title_full | In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging |
| title_fullStr | In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging |
| title_full_unstemmed | In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging |
| title_short | In Vivo Tracking of Systemically Administered Allogeneic Bone Marrow Mesenchymal Stem Cells in Normal Rats through Bioluminescence Imaging |
| title_sort | in vivo tracking of systemically administered allogeneic bone marrow mesenchymal stem cells in normal rats through bioluminescence imaging |
| url | http://dx.doi.org/10.1155/2016/3970942 |
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