Rapid detection of nitrite and Listeria monocytogenes in food based on multiple simulated enzyme activities of Fe3O4-ZnO-MnO2 nanocomposites
Food safety has emerged as a paramount concern in global health, prompting innovative approaches to ensure the safety of people's sustenance. In this study, a novel strategy was devised to fabricate Fe3O4-ZnO-MnO2 hybrid nanobiocatalysts, which exhibited remarkable enzymatic activity surpassing...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524008563 |
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| Summary: | Food safety has emerged as a paramount concern in global health, prompting innovative approaches to ensure the safety of people's sustenance. In this study, a novel strategy was devised to fabricate Fe3O4-ZnO-MnO2 hybrid nanobiocatalysts, which exhibited remarkable enzymatic activity surpassing that of Horseradish peroxidase (HRP) catalysis. It demonstrated exceptional proficiency in decomposing 3,3′,5,5′-tetramethylbenzidine (TMB) without the need for harsh reaction conditions or the aid of H2O2. We established colorimetric detection systems based on Fe3O4-ZnO-MnO2-TMB both for nitrite (NO2−) and Listeria monocytogenes (LM) in food. Impressively, the detection limit of nitrite reached an astonishingly low level of 0.022 mg L−1, and the detection limit for LM was determined to be 3.5 cfu mL−1. These compelling results unequivocally validate the potential of these hybrid nanobiocatalysts to fortify food safety measures. Moreover, they serve as a valuable reference for the colorimetric detection of diverse analytes and the simultaneous detection of multiple targets. |
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| ISSN: | 2590-1575 |