Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice
Osteoporosis (OP) is a prevalent chronic bone metabolic disorder that affects the elderly population, leading to an increased susceptibility to bone fragility. Despite extensive research on the onset and progression of OP, the precise mechanisms underlying this condition remain elusive. The m6A modi...
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025005031 |
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| author | Lifeng Zheng Chao Lan Xinyue Gao An Zhu Yaoqing Chen Jinluan Lin Sunjie Yan Ximei Shen |
| author_facet | Lifeng Zheng Chao Lan Xinyue Gao An Zhu Yaoqing Chen Jinluan Lin Sunjie Yan Ximei Shen |
| author_sort | Lifeng Zheng |
| collection | DOAJ |
| description | Osteoporosis (OP) is a prevalent chronic bone metabolic disorder that affects the elderly population, leading to an increased susceptibility to bone fragility. Despite extensive research on the onset and progression of OP, the precise mechanisms underlying this condition remain elusive. The m6A modification, a prevalent form of chemical RNA modification, primarily regulates posttranscriptional processes, including RNA stability, splicing, and translation. Numerous studies have underscored the crucial functions of m6A regulators in OP. This study aimed to explore the relationship between OP and RNA m6A methylation, investigating its underlying mechanisms through comprehensive bioinformatic analysis and experimental validation. The mRNA sequencing (mRNA-seq) and methylated RNA immunoprecipitation sequencing (MeRIP-seq) were performed on control mice as well as ovariectomized mice to discover differentially expressed genes (DEGs) and m6A regulators in OP. The results revealed dysregulation of a majority of bone metabolism-related genes and m6A regulators in ovariectomized mice, indicating a closely linked relationship between them. Our research findings indicated that m6A modification is essential in regulating OP, offering potential insights for prevention and treatment. |
| format | Article |
| id | doaj-art-9dcdb61133034584b543aab3ce8050a4 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-9dcdb61133034584b543aab3ce8050a42025-02-06T05:12:34ZengElsevierHeliyon2405-84402025-02-01113e42123Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis miceLifeng Zheng0Chao Lan1Xinyue Gao2An Zhu3Yaoqing Chen4Jinluan Lin5Sunjie Yan6Ximei Shen7Department of Orthopedics, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Orthopedics, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, ChinaDepartment of Endocrinology, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, ChinaKey Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, Fuzhou, 350108, ChinaKey Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, Fuzhou, 350108, ChinaDepartment of Orthopedics, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Orthopedics, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, ChinaDepartment of Orthopedics, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Orthopedics, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, ChinaDepartment of Endocrinology, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Endocrinology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, China; Clinical Research Center for Metabolic Diseases of Fujian Province, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Fujian Key Laboratory of Glycolipid and Bone Mineral Metabolism, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Diabetes Research Institute of Fujian Province, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Metabolic Diseases Research Institute, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, ChinaDepartment of Endocrinology, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Department of Endocrinology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, China; Clinical Research Center for Metabolic Diseases of Fujian Province, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Fujian Key Laboratory of Glycolipid and Bone Mineral Metabolism, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Diabetes Research Institute of Fujian Province, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Metabolic Diseases Research Institute, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China; Corresponding author. Department of Endocrinology, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China.Osteoporosis (OP) is a prevalent chronic bone metabolic disorder that affects the elderly population, leading to an increased susceptibility to bone fragility. Despite extensive research on the onset and progression of OP, the precise mechanisms underlying this condition remain elusive. The m6A modification, a prevalent form of chemical RNA modification, primarily regulates posttranscriptional processes, including RNA stability, splicing, and translation. Numerous studies have underscored the crucial functions of m6A regulators in OP. This study aimed to explore the relationship between OP and RNA m6A methylation, investigating its underlying mechanisms through comprehensive bioinformatic analysis and experimental validation. The mRNA sequencing (mRNA-seq) and methylated RNA immunoprecipitation sequencing (MeRIP-seq) were performed on control mice as well as ovariectomized mice to discover differentially expressed genes (DEGs) and m6A regulators in OP. The results revealed dysregulation of a majority of bone metabolism-related genes and m6A regulators in ovariectomized mice, indicating a closely linked relationship between them. Our research findings indicated that m6A modification is essential in regulating OP, offering potential insights for prevention and treatment.http://www.sciencedirect.com/science/article/pii/S2405844025005031OsteoporosisBone metabolismOvariectomyOsteoporosis animal modelsm6A methylation |
| spellingShingle | Lifeng Zheng Chao Lan Xinyue Gao An Zhu Yaoqing Chen Jinluan Lin Sunjie Yan Ximei Shen Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice Heliyon Osteoporosis Bone metabolism Ovariectomy Osteoporosis animal models m6A methylation |
| title | Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice |
| title_full | Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice |
| title_fullStr | Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice |
| title_full_unstemmed | Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice |
| title_short | Landscape analysis of m6A modification reveals the dysfunction of bone metabolism in osteoporosis mice |
| title_sort | landscape analysis of m6a modification reveals the dysfunction of bone metabolism in osteoporosis mice |
| topic | Osteoporosis Bone metabolism Ovariectomy Osteoporosis animal models m6A methylation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025005031 |
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