Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum
Background Preeclampsia (PE) is characterized as de novo hypertension (HTN) with end-organ damage, especially in the brain. PE is hypothesized to be caused by placental ischemia. PE affects ~5–8% of USA pregnancies and increases the risk for HTN and cerebrovascular diseases (CVD) later in life. We h...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Hypertension in Pregnancy |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10641955.2025.2454597 |
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| author | Savanna Smith Jonna Smith Kylie Jones Angie Castillo Natalia Wiemann Ahfiya Howard Mark Cunningham |
| author_facet | Savanna Smith Jonna Smith Kylie Jones Angie Castillo Natalia Wiemann Ahfiya Howard Mark Cunningham |
| author_sort | Savanna Smith |
| collection | DOAJ |
| description | Background Preeclampsia (PE) is characterized as de novo hypertension (HTN) with end-organ damage, especially in the brain. PE is hypothesized to be caused by placental ischemia. PE affects ~5–8% of USA pregnancies and increases the risk for HTN and cerebrovascular diseases (CVD) later in life. We hypothesize that blood pressure (BP), cerebral oxidative stress, and cerebral inflammation will increase in postpartum (PP) placental ischemic dams.Methods Placental ischemia was induced in pregnant Sprague Dawley dams, utilizing reduced uterine perfusion pressure (RUPP) surgery. At 6 weeks PP (~3 human years), BP was measured via carotid catheterization, and cerebral oxidative stress and inflammation were assessed via ELISAs, biochemical assays, and Western blots.Results BP, cerebral pro-inflammatory cytokines (TNF-α and IL-6), and GFAP (a marker of astrocyte activity) were increased in PP RUPP dams. Cerebral hydrogen peroxide (H2O2) was also increased in PP RUPP dams, and had a strong correlation with PP RUPP BP, proinflammatory cytokines (TNF- α and IL-6), and GFAP astrocyte activation.Conclusion PP RUPP dams have increased BP, cerebral oxidative stress, and cerebral inflammation at 6 weeks postpartum. These changes in cerebral inflammation and oxidative stress may contribute to the pathology and development of HTN and CVDs in postpartum dams. |
| format | Article |
| id | doaj-art-9dc668e7dc9e42469573e917e72d0b76 |
| institution | Kabale University |
| issn | 1064-1955 1525-6065 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hypertension in Pregnancy |
| spelling | doaj-art-9dc668e7dc9e42469573e917e72d0b762025-01-31T04:44:23ZengTaylor & Francis GroupHypertension in Pregnancy1064-19551525-60652025-12-0144110.1080/10641955.2025.2454597Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartumSavanna Smith0Jonna Smith1Kylie Jones2Angie Castillo3Natalia Wiemann4Ahfiya Howard5Mark Cunningham6Department of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USADepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USADepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USADepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USATexas College of Osteopathic Medicine, University of North Texas Health Science Center, Fort Worth, TX, USASchool of Social Work, Texas A & M University-Commerce, Commerce, TX, USADepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USABackground Preeclampsia (PE) is characterized as de novo hypertension (HTN) with end-organ damage, especially in the brain. PE is hypothesized to be caused by placental ischemia. PE affects ~5–8% of USA pregnancies and increases the risk for HTN and cerebrovascular diseases (CVD) later in life. We hypothesize that blood pressure (BP), cerebral oxidative stress, and cerebral inflammation will increase in postpartum (PP) placental ischemic dams.Methods Placental ischemia was induced in pregnant Sprague Dawley dams, utilizing reduced uterine perfusion pressure (RUPP) surgery. At 6 weeks PP (~3 human years), BP was measured via carotid catheterization, and cerebral oxidative stress and inflammation were assessed via ELISAs, biochemical assays, and Western blots.Results BP, cerebral pro-inflammatory cytokines (TNF-α and IL-6), and GFAP (a marker of astrocyte activity) were increased in PP RUPP dams. Cerebral hydrogen peroxide (H2O2) was also increased in PP RUPP dams, and had a strong correlation with PP RUPP BP, proinflammatory cytokines (TNF- α and IL-6), and GFAP astrocyte activation.Conclusion PP RUPP dams have increased BP, cerebral oxidative stress, and cerebral inflammation at 6 weeks postpartum. These changes in cerebral inflammation and oxidative stress may contribute to the pathology and development of HTN and CVDs in postpartum dams.https://www.tandfonline.com/doi/10.1080/10641955.2025.2454597Preeclampsiapostpartuminflammationoxidative stresshypertension |
| spellingShingle | Savanna Smith Jonna Smith Kylie Jones Angie Castillo Natalia Wiemann Ahfiya Howard Mark Cunningham Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum Hypertension in Pregnancy Preeclampsia postpartum inflammation oxidative stress hypertension |
| title | Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum |
| title_full | Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum |
| title_fullStr | Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum |
| title_full_unstemmed | Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum |
| title_short | Placental ischemia during pregnancy induces hypertension, cerebral inflammation, and oxidative stress in dams postpartum |
| title_sort | placental ischemia during pregnancy induces hypertension cerebral inflammation and oxidative stress in dams postpartum |
| topic | Preeclampsia postpartum inflammation oxidative stress hypertension |
| url | https://www.tandfonline.com/doi/10.1080/10641955.2025.2454597 |
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