Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males
This study focuses on the regulatory effects of genes encoding the juvenile hormone (JH) receptor methoprene-tolerant (<i>Met</i>) and transcription factor krüppel homolog 1 (<i>Kr-h1</i>) on the reproductive capacity of <i>Coccinella septempunctata</i> male adult...
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2025-01-01
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| author | Ying Cheng Yuhang Zhou Cao Li |
| author_facet | Ying Cheng Yuhang Zhou Cao Li |
| author_sort | Ying Cheng |
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| description | This study focuses on the regulatory effects of genes encoding the juvenile hormone (JH) receptor methoprene-tolerant (<i>Met</i>) and transcription factor krüppel homolog 1 (<i>Kr-h1</i>) on the reproductive capacity of <i>Coccinella septempunctata</i> male adults. <i>Met</i> and <i>Kr-h1</i> expression levels were analyzed in males fed on artificial diets with and without JH by quantitative real-time PCR, and the effects of <i>Met</i> and <i>Kr-h1</i> on male reproduction were analyzed by RNA interference technology. <i>Met</i> transcription levels in 5- and 10-day-old males fed with a JH-supplemented diet were lower than those without JH. <i>Kr-h1</i> expression in 5-day-old adult males was lower in diets lacking JH but was higher in 10-day-old males fed on a diet lacking JH. There were no significant differences in the testes sizes of male ladybugs injected with <i>Met</i>-dsRNA when compared to <i>GFP</i>-dsRNA; however, the testis volume of ladybugs injected with <i>Kr-h1</i>-dsRNA was smaller than those injected with <i>GFP</i>-dsRNA. After males were injected with <i>Met</i>-dsRNA and <i>Kr-h1</i>-dsRNA, the mean egg production by females decreased by 12.75% and 23.10%, respectively, at 20 d postinjection. Our results show that <i>Met</i> and <i>Kr-h1</i> have important roles in regulating reproduction by directly affecting testes development in males and egg production in females. |
| format | Article |
| id | doaj-art-9dc3b820c81248699e9e1b03346b6122 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-9dc3b820c81248699e9e1b03346b61222025-01-24T13:35:43ZengMDPI AGInsects2075-44502025-01-011614910.3390/insects16010049Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> MalesYing Cheng0Yuhang Zhou1Cao Li2Guizhou Institute of Plant Protection, Guiyang 550006, ChinaGuizhou Institute of Plant Protection, Guiyang 550006, ChinaGuizhou Provincial Pollution-Free Engineering Center of Plant Protection, Guiyang 550006, ChinaThis study focuses on the regulatory effects of genes encoding the juvenile hormone (JH) receptor methoprene-tolerant (<i>Met</i>) and transcription factor krüppel homolog 1 (<i>Kr-h1</i>) on the reproductive capacity of <i>Coccinella septempunctata</i> male adults. <i>Met</i> and <i>Kr-h1</i> expression levels were analyzed in males fed on artificial diets with and without JH by quantitative real-time PCR, and the effects of <i>Met</i> and <i>Kr-h1</i> on male reproduction were analyzed by RNA interference technology. <i>Met</i> transcription levels in 5- and 10-day-old males fed with a JH-supplemented diet were lower than those without JH. <i>Kr-h1</i> expression in 5-day-old adult males was lower in diets lacking JH but was higher in 10-day-old males fed on a diet lacking JH. There were no significant differences in the testes sizes of male ladybugs injected with <i>Met</i>-dsRNA when compared to <i>GFP</i>-dsRNA; however, the testis volume of ladybugs injected with <i>Kr-h1</i>-dsRNA was smaller than those injected with <i>GFP</i>-dsRNA. After males were injected with <i>Met</i>-dsRNA and <i>Kr-h1</i>-dsRNA, the mean egg production by females decreased by 12.75% and 23.10%, respectively, at 20 d postinjection. Our results show that <i>Met</i> and <i>Kr-h1</i> have important roles in regulating reproduction by directly affecting testes development in males and egg production in females.https://www.mdpi.com/2075-4450/16/1/49<i>Coccinella septempunctata</i>juvenile hormone<i>methoprene-tolerant</i><i>krüppel homolog 1</i>RNAireproductive regulation |
| spellingShingle | Ying Cheng Yuhang Zhou Cao Li Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males Insects <i>Coccinella septempunctata</i> juvenile hormone <i>methoprene-tolerant</i> <i>krüppel homolog 1</i> RNAi reproductive regulation |
| title | Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males |
| title_full | Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males |
| title_fullStr | Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males |
| title_full_unstemmed | Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males |
| title_short | Functional Analysis of Genes Encoding Juvenile Hormone Receptor <i>Met</i> and Transcription Factor <i>Kr-h1</i> in the Reproductive Capacity of <i>Coccinella septempunctata</i> Males |
| title_sort | functional analysis of genes encoding juvenile hormone receptor i met i and transcription factor i kr h1 i in the reproductive capacity of i coccinella septempunctata i males |
| topic | <i>Coccinella septempunctata</i> juvenile hormone <i>methoprene-tolerant</i> <i>krüppel homolog 1</i> RNAi reproductive regulation |
| url | https://www.mdpi.com/2075-4450/16/1/49 |
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