Preliminary Biological Assessments of Some Algae Basis Biomaterials

Four types of algae—<i>Porphyra umbilicalis</i>, <i>Undaria pinnatifida</i>, <i>Cystoseira barbata</i>, and <i>Chlorella</i> sp.—were used to obtain crude bioproducts enriched in polysaccharides (four bioproducts) and to create formulations enriched wi...

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Main Authors: Toader Marian, Nicoleta Radu, Mariana Voicescu, Cristina Lavinia Nistor, Lucia Camelia Pirvu, Mirela Mihaila, Marinela Bostan
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Journal of Marine Science and Engineering
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Online Access:https://www.mdpi.com/2077-1312/13/2/318
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author Toader Marian
Nicoleta Radu
Mariana Voicescu
Cristina Lavinia Nistor
Lucia Camelia Pirvu
Mirela Mihaila
Marinela Bostan
author_facet Toader Marian
Nicoleta Radu
Mariana Voicescu
Cristina Lavinia Nistor
Lucia Camelia Pirvu
Mirela Mihaila
Marinela Bostan
author_sort Toader Marian
collection DOAJ
description Four types of algae—<i>Porphyra umbilicalis</i>, <i>Undaria pinnatifida</i>, <i>Cystoseira barbata</i>, and <i>Chlorella</i> sp.—were used to obtain crude bioproducts enriched in polysaccharides (four bioproducts) and to create formulations enriched with gold cations (four bioproducts). The bioproducts obtained through aqueous extraction from <i>Cystoseira barbata</i> exhibited significant antioxidant activities and a total polyphenol content of (714.17 ± 1.26) mg GAE/L. In the bioproducts derived from the aqueous extract of <i>Porphyra umbilicalis</i> and <i>Undaria pinnatifida</i>, combined with gold ions, gold nanoparticles with sizes of less than 34 nm were formed. In vitro tests performed on the Caco-2 tumour cell line with each of the eight bioproducts, after 24 h of exposure, showed that the crude bioproducts containing polysaccharides derived from <i>Porphyra umbilicalis</i>, <i>Undaria pinnatifida</i>, and <i>Chlorella</i> sp. exhibited cytotoxicity against the Caco-2 cell line. In the case of the HepG2 cell line, after 24 h of exposure, the tests indicated that only the crude polysaccharides derived from <i>Cystoseira barbata</i> exhibited cytotoxic effects. These results indicate the protective effect of the algal polysaccharides against the tumourigenesis processes that may occur in the human digestive system. Regarding the bioproducts containing gold, no cytotoxic effect was observed. However, in the case of the two algal bioproducts containing gold nanoparticles with a size of less than 34 nm, they may represent potential raw materials for electrochemical sensors.
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spelling doaj-art-9dbc3d19463f40e2bc4d1d05b5a9af932025-08-20T02:44:32ZengMDPI AGJournal of Marine Science and Engineering2077-13122025-02-0113231810.3390/jmse13020318Preliminary Biological Assessments of Some Algae Basis BiomaterialsToader Marian0Nicoleta Radu1Mariana Voicescu2Cristina Lavinia Nistor3Lucia Camelia Pirvu4Mirela Mihaila5Marinela Bostan6Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Boulevard, District 1, 011464 Bucharest, RomaniaFaculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Boulevard, District 1, 011464 Bucharest, RomaniaDepartment of Quantum Chemistry and Molecular Structure, Institute of Physical Chemistry “Ilie Murgulescu”, 202 Splaiul Independentei St. 060021, 060021 Bucharest, RomaniaDepartment of Biotechnologies, National Institute of Chemistry and Petrochemistry R&D of Bucharest, 202 Splaiul Independentei Street, District 6, 060021 Bucharest, RomaniaDepartment of Pharmaceutical Biotechnologies, National Institute of Chemical Pharmaceutical Research and Development, 112 Vitan, 031299 Bucharest, RomaniaCenter of Immunology, Institute of Virology Stefan S, Nicolau, 285 Mihai Bravu Avenue, 030304 Bucharest, RomaniaCenter of Immunology, Institute of Virology Stefan S, Nicolau, 285 Mihai Bravu Avenue, 030304 Bucharest, RomaniaFour types of algae—<i>Porphyra umbilicalis</i>, <i>Undaria pinnatifida</i>, <i>Cystoseira barbata</i>, and <i>Chlorella</i> sp.—were used to obtain crude bioproducts enriched in polysaccharides (four bioproducts) and to create formulations enriched with gold cations (four bioproducts). The bioproducts obtained through aqueous extraction from <i>Cystoseira barbata</i> exhibited significant antioxidant activities and a total polyphenol content of (714.17 ± 1.26) mg GAE/L. In the bioproducts derived from the aqueous extract of <i>Porphyra umbilicalis</i> and <i>Undaria pinnatifida</i>, combined with gold ions, gold nanoparticles with sizes of less than 34 nm were formed. In vitro tests performed on the Caco-2 tumour cell line with each of the eight bioproducts, after 24 h of exposure, showed that the crude bioproducts containing polysaccharides derived from <i>Porphyra umbilicalis</i>, <i>Undaria pinnatifida</i>, and <i>Chlorella</i> sp. exhibited cytotoxicity against the Caco-2 cell line. In the case of the HepG2 cell line, after 24 h of exposure, the tests indicated that only the crude polysaccharides derived from <i>Cystoseira barbata</i> exhibited cytotoxic effects. These results indicate the protective effect of the algal polysaccharides against the tumourigenesis processes that may occur in the human digestive system. Regarding the bioproducts containing gold, no cytotoxic effect was observed. However, in the case of the two algal bioproducts containing gold nanoparticles with a size of less than 34 nm, they may represent potential raw materials for electrochemical sensors.https://www.mdpi.com/2077-1312/13/2/318aqueous extractalgaegold nanoparticlecytotoxicity
spellingShingle Toader Marian
Nicoleta Radu
Mariana Voicescu
Cristina Lavinia Nistor
Lucia Camelia Pirvu
Mirela Mihaila
Marinela Bostan
Preliminary Biological Assessments of Some Algae Basis Biomaterials
Journal of Marine Science and Engineering
aqueous extract
algae
gold nanoparticle
cytotoxicity
title Preliminary Biological Assessments of Some Algae Basis Biomaterials
title_full Preliminary Biological Assessments of Some Algae Basis Biomaterials
title_fullStr Preliminary Biological Assessments of Some Algae Basis Biomaterials
title_full_unstemmed Preliminary Biological Assessments of Some Algae Basis Biomaterials
title_short Preliminary Biological Assessments of Some Algae Basis Biomaterials
title_sort preliminary biological assessments of some algae basis biomaterials
topic aqueous extract
algae
gold nanoparticle
cytotoxicity
url https://www.mdpi.com/2077-1312/13/2/318
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AT nicoletaradu preliminarybiologicalassessmentsofsomealgaebasisbiomaterials
AT marianavoicescu preliminarybiologicalassessmentsofsomealgaebasisbiomaterials
AT cristinalavinianistor preliminarybiologicalassessmentsofsomealgaebasisbiomaterials
AT luciacameliapirvu preliminarybiologicalassessmentsofsomealgaebasisbiomaterials
AT mirelamihaila preliminarybiologicalassessmentsofsomealgaebasisbiomaterials
AT marinelabostan preliminarybiologicalassessmentsofsomealgaebasisbiomaterials