Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species
Abstract Human gut Bacteroides and Parabacteroides species play crucial roles in human health and are known for their capacity to utilize diverse polysaccharides. Understanding how these bacteria utilize medicinal polysaccharides is foundational for developing polysaccharides-based prebiotics and dr...
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-55845-7 |
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| author | Zepeng Qu Hongbin Liu Ji Yang Linggang Zheng Jumin Huang Ziming Wang Chun Xie Wenlong Zuo Xiong Xia Lin Sun Yifa Zhou Ying Xie Jingguang Lu Yizhun Zhu Lili Yu Lihua Liu Hua Zhou Lei Dai Elaine Lai-Han Leung |
| author_facet | Zepeng Qu Hongbin Liu Ji Yang Linggang Zheng Jumin Huang Ziming Wang Chun Xie Wenlong Zuo Xiong Xia Lin Sun Yifa Zhou Ying Xie Jingguang Lu Yizhun Zhu Lili Yu Lihua Liu Hua Zhou Lei Dai Elaine Lai-Han Leung |
| author_sort | Zepeng Qu |
| collection | DOAJ |
| description | Abstract Human gut Bacteroides and Parabacteroides species play crucial roles in human health and are known for their capacity to utilize diverse polysaccharides. Understanding how these bacteria utilize medicinal polysaccharides is foundational for developing polysaccharides-based prebiotics and drugs. Here, we systematically mapped the utilization profiles of 20 different medicinal polysaccharides by 28 human gut Bacteroides and Parabacteroides species. The growth profiles exhibited substantial variation across different bacterial species and medicinal polysaccharides. Ginseng polysaccharides promoted the growth of multiple Bacteroides and Parabacteroides species; in contrast, Dendrobium polysaccharides selectively promoted the growth of Bacteroides uniformis. This distinct utilization profile was associated with genomic variation in carbohydrate-active enzymes, rather than monosaccharides composition variation among medicinal polysaccharides. Through comparative transcriptomics and genetical manipulation, we validated that the polysaccharide utilization locus PUL34_Bu enabled Bacteroides uniformis to utilize Dendrobium polysaccharides (i.e. glucomannan). In addition, we found that the GH26 enzyme in PUL34_Bu allowed Bacteroides uniformis to utilize multiple plant-derived mannan. Overall, our results revealed the selective utilization of medicinal polysaccharide by Bacteroides and Parabacteroides species and provided insights into the use of polysaccharides in engineering the human gut microbiome. |
| format | Article |
| id | doaj-art-9db5964d173b44ca98ce655ef6c3303a |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-9db5964d173b44ca98ce655ef6c3303a2025-02-02T12:31:58ZengNature PortfolioNature Communications2041-17232025-01-0116111710.1038/s41467-025-55845-7Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides speciesZepeng Qu0Hongbin Liu1Ji Yang2Linggang Zheng3Jumin Huang4Ziming Wang5Chun Xie6Wenlong Zuo7Xiong Xia8Lin Sun9Yifa Zhou10Ying Xie11Jingguang Lu12Yizhun Zhu13Lili Yu14Lihua Liu15Hua Zhou16Lei Dai17Elaine Lai-Han Leung18School of Pharmacy, Faculty of Medicine & State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, TaipaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesSchool of Chinese Materia Medica, Guangdong Pharmaceutical UniversitySchool of Pharmacy, Faculty of Medicine & State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, TaipaCancer Center, Faculty of Health Sciences, Ministry of Education (MOE) Frontiers Science Center for Precision Oncology, University of Macau, MacauCancer Center, Faculty of Health Sciences, Ministry of Education (MOE) Frontiers Science Center for Precision Oncology, University of Macau, MacauCancer Center, Faculty of Health Sciences, Ministry of Education (MOE) Frontiers Science Center for Precision Oncology, University of Macau, MacauCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesJilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, Northeast Normal UniversityJilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, Northeast Normal UniversityState Key Laboratory of Traditional Chinese Medicine Syndrome, Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, GuangzhouSchool of Pharmacy, Faculty of Medicine & State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, TaipaSchool of Pharmacy, Faculty of Medicine & State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, TaipaSchool of Pharmacy, Faculty of Medicine & State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, TaipaSchool of Economics and Management, Yanbian UniversityState Key Laboratory of Traditional Chinese Medicine Syndrome, Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, GuangzhouCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesCancer Center, Faculty of Health Sciences, Ministry of Education (MOE) Frontiers Science Center for Precision Oncology, University of Macau, MacauAbstract Human gut Bacteroides and Parabacteroides species play crucial roles in human health and are known for their capacity to utilize diverse polysaccharides. Understanding how these bacteria utilize medicinal polysaccharides is foundational for developing polysaccharides-based prebiotics and drugs. Here, we systematically mapped the utilization profiles of 20 different medicinal polysaccharides by 28 human gut Bacteroides and Parabacteroides species. The growth profiles exhibited substantial variation across different bacterial species and medicinal polysaccharides. Ginseng polysaccharides promoted the growth of multiple Bacteroides and Parabacteroides species; in contrast, Dendrobium polysaccharides selectively promoted the growth of Bacteroides uniformis. This distinct utilization profile was associated with genomic variation in carbohydrate-active enzymes, rather than monosaccharides composition variation among medicinal polysaccharides. Through comparative transcriptomics and genetical manipulation, we validated that the polysaccharide utilization locus PUL34_Bu enabled Bacteroides uniformis to utilize Dendrobium polysaccharides (i.e. glucomannan). In addition, we found that the GH26 enzyme in PUL34_Bu allowed Bacteroides uniformis to utilize multiple plant-derived mannan. Overall, our results revealed the selective utilization of medicinal polysaccharide by Bacteroides and Parabacteroides species and provided insights into the use of polysaccharides in engineering the human gut microbiome.https://doi.org/10.1038/s41467-025-55845-7 |
| spellingShingle | Zepeng Qu Hongbin Liu Ji Yang Linggang Zheng Jumin Huang Ziming Wang Chun Xie Wenlong Zuo Xiong Xia Lin Sun Yifa Zhou Ying Xie Jingguang Lu Yizhun Zhu Lili Yu Lihua Liu Hua Zhou Lei Dai Elaine Lai-Han Leung Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species Nature Communications |
| title | Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species |
| title_full | Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species |
| title_fullStr | Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species |
| title_full_unstemmed | Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species |
| title_short | Selective utilization of medicinal polysaccharides by human gut Bacteroides and Parabacteroides species |
| title_sort | selective utilization of medicinal polysaccharides by human gut bacteroides and parabacteroides species |
| url | https://doi.org/10.1038/s41467-025-55845-7 |
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