Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide
Models of Parkinson’s disease with neurotoxins have shown that microglial activation does not evoke a typical inflammatory response in the substantia nigra, questioning whether neuroinflammation leads to neurodegeneration. To address this issue, the archetypal inflammatory stimulus, lipopolysacchari...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/1838921 |
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| author | Yazmin M. Flores-Martinez Manuel A. Fernandez-Parrilla Jose Ayala-Davila David Reyes-Corona Victor M. Blanco-Alvarez Luis O. Soto-Rojas Claudia Luna-Herrera Juan A. Gonzalez-Barrios Bertha A. Leon-Chavez Maria E. Gutierrez-Castillo Irma A. Martínez-Dávila Daniel Martinez-Fong |
| author_facet | Yazmin M. Flores-Martinez Manuel A. Fernandez-Parrilla Jose Ayala-Davila David Reyes-Corona Victor M. Blanco-Alvarez Luis O. Soto-Rojas Claudia Luna-Herrera Juan A. Gonzalez-Barrios Bertha A. Leon-Chavez Maria E. Gutierrez-Castillo Irma A. Martínez-Dávila Daniel Martinez-Fong |
| author_sort | Yazmin M. Flores-Martinez |
| collection | DOAJ |
| description | Models of Parkinson’s disease with neurotoxins have shown that microglial activation does not evoke a typical inflammatory response in the substantia nigra, questioning whether neuroinflammation leads to neurodegeneration. To address this issue, the archetypal inflammatory stimulus, lipopolysaccharide (LPS), was injected into the rat substantia nigra. LPS induced fever, sickness behavior, and microglial activation (OX42 immunoreactivity), followed by astrocyte activation and leukocyte infiltration (GFAP and CD45 immunoreactivities). During the acute phase of neuroinflammation, pro- and anti-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-4, and IL-10) responded differentially at mRNA and protein level. Increased NO production and lipid peroxidation occurred at 168 h after LPS injection. At this time, evidence of neurodegeneration could be seen, entailing decreased tyrosine hydroxylase (TH) immunoreactivity, irregular body contour, and prolongation discontinuity of TH+ cells, as well as apparent phagocytosis of TH+ cells by OX42+ cells. Altogether, these results show that LPS evokes a typical inflammatory response in the substantia nigra that is followed by dopaminergic neurodegeneration. |
| format | Article |
| id | doaj-art-9db253425860463ca059a8ea37387a12 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9db253425860463ca059a8ea37387a122025-02-03T01:00:03ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/18389211838921Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of LipopolysaccharideYazmin M. Flores-Martinez0Manuel A. Fernandez-Parrilla1Jose Ayala-Davila2David Reyes-Corona3Victor M. Blanco-Alvarez4Luis O. Soto-Rojas5Claudia Luna-Herrera6Juan A. Gonzalez-Barrios7Bertha A. Leon-Chavez8Maria E. Gutierrez-Castillo9Irma A. Martínez-Dávila10Daniel Martinez-Fong11Departamento de Fisiología, Biofísica y Neurociencias, Av. Instituto Politécnico Nacional No. 2508, San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados, 07360 Ciudad de México, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Av. Instituto Politécnico Nacional No. 2508, San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados, 07360 Ciudad de México, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Av. Instituto Politécnico Nacional No. 2508, San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados, 07360 Ciudad de México, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Av. Instituto Politécnico Nacional No. 2508, San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados, 07360 Ciudad de México, MexicoFacultad de Ciencias Químicas, 14 Sur y Avenida San Claudio, Benemérita Universidad Autónoma de Puebla, 72570 Puebla, PUE, MexicoDepartamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio y Plan de Ayala s/n, Santo Tomás, Instituto Politécnico Nacional, 11340 Ciudad de México, MexicoDepartamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio y Plan de Ayala s/n, Santo Tomás, Instituto Politécnico Nacional, 11340 Ciudad de México, MexicoLaboratorio de Medicina Genómica, Hospital Regional 1° de Octubre, Avenida Instituto Politécnico Nacional No. 1669, Lindavista, ISSSTE, 07300 Ciudad de México, MexicoFacultad de Ciencias Químicas, 14 Sur y Avenida San Claudio, Benemérita Universidad Autónoma de Puebla, 72570 Puebla, PUE, MexicoDepartamento de Biociencias e Ingeniería, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, 30 de Junio de 1520 s/n, La Laguna Ticoman, Instituto Politécnico Nacional, 07340 Ciudad de México, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Av. Instituto Politécnico Nacional No. 2508, San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados, 07360 Ciudad de México, MexicoDepartamento de Fisiología, Biofísica y Neurociencias, Av. Instituto Politécnico Nacional No. 2508, San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados, 07360 Ciudad de México, MexicoModels of Parkinson’s disease with neurotoxins have shown that microglial activation does not evoke a typical inflammatory response in the substantia nigra, questioning whether neuroinflammation leads to neurodegeneration. To address this issue, the archetypal inflammatory stimulus, lipopolysaccharide (LPS), was injected into the rat substantia nigra. LPS induced fever, sickness behavior, and microglial activation (OX42 immunoreactivity), followed by astrocyte activation and leukocyte infiltration (GFAP and CD45 immunoreactivities). During the acute phase of neuroinflammation, pro- and anti-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-4, and IL-10) responded differentially at mRNA and protein level. Increased NO production and lipid peroxidation occurred at 168 h after LPS injection. At this time, evidence of neurodegeneration could be seen, entailing decreased tyrosine hydroxylase (TH) immunoreactivity, irregular body contour, and prolongation discontinuity of TH+ cells, as well as apparent phagocytosis of TH+ cells by OX42+ cells. Altogether, these results show that LPS evokes a typical inflammatory response in the substantia nigra that is followed by dopaminergic neurodegeneration.http://dx.doi.org/10.1155/2018/1838921 |
| spellingShingle | Yazmin M. Flores-Martinez Manuel A. Fernandez-Parrilla Jose Ayala-Davila David Reyes-Corona Victor M. Blanco-Alvarez Luis O. Soto-Rojas Claudia Luna-Herrera Juan A. Gonzalez-Barrios Bertha A. Leon-Chavez Maria E. Gutierrez-Castillo Irma A. Martínez-Dávila Daniel Martinez-Fong Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide Journal of Immunology Research |
| title | Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide |
| title_full | Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide |
| title_fullStr | Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide |
| title_full_unstemmed | Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide |
| title_short | Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide |
| title_sort | acute neuroinflammatory response in the substantia nigra pars compacta of rats after a local injection of lipopolysaccharide |
| url | http://dx.doi.org/10.1155/2018/1838921 |
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