Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses
Background. Mesangial cells play a prominent role in the development of inflammatory diseases and autoimmune disorders of the kidney. Mesangial cells perform the essential functions of helping to ensure that the glomerular structure is stable and regulating capillary flow, and activated mesangial ce...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/2121849 |
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| author | Hongyu Yu Shaoyuan Cui Yan Mei Qinggang Li Lingling Wu Shuwei Duan Guangyan Cai Hanyu Zhu Bo Fu Li Zhang Zhe Feng Xiangmei Chen |
| author_facet | Hongyu Yu Shaoyuan Cui Yan Mei Qinggang Li Lingling Wu Shuwei Duan Guangyan Cai Hanyu Zhu Bo Fu Li Zhang Zhe Feng Xiangmei Chen |
| author_sort | Hongyu Yu |
| collection | DOAJ |
| description | Background. Mesangial cells play a prominent role in the development of inflammatory diseases and autoimmune disorders of the kidney. Mesangial cells perform the essential functions of helping to ensure that the glomerular structure is stable and regulating capillary flow, and activated mesangial cells acquire proinflammatory activities. We investigated whether activated mesangial cells display immune properties and control the development of T cell immunity. Methods. Flow cytometry analysis was used to study the expression of antigen-presenting cell surface markers and costimulatory molecules in mesangial cells. CD4+ T cell activation induced by mesangial cells was detected in terms of T cell proliferation and cytokine production. Results. IFN-γ-treated mesangial cells express membrane proteins involved in antigen presentation and T cell activation, including MHC-II, ICAM-1, CD40, and CD80. This finding suggests that activated mesangial cells can take up and present antigenic peptides to initiate CD4+ T cell responses and thus act as nonprofessional antigen-presenting cells. Polarization of naïve CD4+ T cells (Th0 cells) towards the Th1 phenotype was induced by coculture with activated mesangial cells, and the resulting Th1 cells showed increased mRNA and protein expression of inflammation-associated genes. Conclusion. Mesangial cells can present antigen and modulate CD4+ T lymphocyte proliferation and differentiation. Interactions between mesangial cells and T cells are essential for sustaining the inflammatory response in a variety of glomerulonephritides. Therefore, mesangial cells might participate in immune function in the kidney. |
| format | Article |
| id | doaj-art-9d7e8878dde94983a49d76c0c0c3bcd9 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9d7e8878dde94983a49d76c0c0c3bcd92025-02-03T01:23:05ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/21218492121849Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell ResponsesHongyu Yu0Shaoyuan Cui1Yan Mei2Qinggang Li3Lingling Wu4Shuwei Duan5Guangyan Cai6Hanyu Zhu7Bo Fu8Li Zhang9Zhe Feng10Xiangmei Chen11Department of Nephrology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, ChinaDepartment of Nephrology, The Second Hospital of Jilin University, Changchun, Jilin, ChinaBackground. Mesangial cells play a prominent role in the development of inflammatory diseases and autoimmune disorders of the kidney. Mesangial cells perform the essential functions of helping to ensure that the glomerular structure is stable and regulating capillary flow, and activated mesangial cells acquire proinflammatory activities. We investigated whether activated mesangial cells display immune properties and control the development of T cell immunity. Methods. Flow cytometry analysis was used to study the expression of antigen-presenting cell surface markers and costimulatory molecules in mesangial cells. CD4+ T cell activation induced by mesangial cells was detected in terms of T cell proliferation and cytokine production. Results. IFN-γ-treated mesangial cells express membrane proteins involved in antigen presentation and T cell activation, including MHC-II, ICAM-1, CD40, and CD80. This finding suggests that activated mesangial cells can take up and present antigenic peptides to initiate CD4+ T cell responses and thus act as nonprofessional antigen-presenting cells. Polarization of naïve CD4+ T cells (Th0 cells) towards the Th1 phenotype was induced by coculture with activated mesangial cells, and the resulting Th1 cells showed increased mRNA and protein expression of inflammation-associated genes. Conclusion. Mesangial cells can present antigen and modulate CD4+ T lymphocyte proliferation and differentiation. Interactions between mesangial cells and T cells are essential for sustaining the inflammatory response in a variety of glomerulonephritides. Therefore, mesangial cells might participate in immune function in the kidney.http://dx.doi.org/10.1155/2019/2121849 |
| spellingShingle | Hongyu Yu Shaoyuan Cui Yan Mei Qinggang Li Lingling Wu Shuwei Duan Guangyan Cai Hanyu Zhu Bo Fu Li Zhang Zhe Feng Xiangmei Chen Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses Journal of Immunology Research |
| title | Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses |
| title_full | Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses |
| title_fullStr | Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses |
| title_full_unstemmed | Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses |
| title_short | Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses |
| title_sort | mesangial cells exhibit features of antigen presenting cells and activate cd4 t cell responses |
| url | http://dx.doi.org/10.1155/2019/2121849 |
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