Identifying the potential of ovalbumin and its’ hydrolysates to be used in a liquid food system to develop functional beverages

Ready-to-drink protein beverages have gained attention in the functional beverage market. This study examined the possibility of using ovalbumin and its hydrolysates to develop functional protein beverages. Lyophilized ovalbumin (5%, w/v) was dissolved in distilled water (pH 10) with sugar, salt, an...

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Bibliographic Details
Main Authors: Maggonage Hasini Udeshika Maggonage, Prabudhdha Manjula, Edirisingha Dewage Nalaka Sandun Abeyrathne, Dewei Shu, Xi Huang, Dong Uk Ahn
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579124010939
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Summary:Ready-to-drink protein beverages have gained attention in the functional beverage market. This study examined the possibility of using ovalbumin and its hydrolysates to develop functional protein beverages. Lyophilized ovalbumin (5%, w/v) was dissolved in distilled water (pH 10) with sugar, salt, and vanilla and pasteurized at 56.7°C for 3.5 min to prepare a basal ovalbumin beverage (OVA). The beverages were sequentially hydrolyzed using pepsin (pH 2.5, 37°C for 3 h) first, then with protease (pH 6.5, 37°C for 3 h), or protease (pH 6.5, 37°C for 3 h) followed by trypsin (pH 7.8, 37°C for 3 h) combinations. Three beverages (OVA, PrTr, and PePr) were tested for physical, functional, and quality characteristics. Complete hydrolysis by enzyme combinations was ensured using 15% SDS-PAGE. Enzymatic hydrolysis caused an increase in the solubility of ovalbumin in water. The beverages showed antioxidant activity, and hydrolyzed drinks showed antimicrobial activity. No significant difference in acidity, but a reduction in pH, an increase in TSS, and alterations in viscosity were observed among the drinks with storage. A significant difference (p < 0.05) in titratable acidity was identified between the two hydrolyzed drinks, but no difference (p > 0.05) within a treatment was observed until Day 14. In conclusion, ovalbumin and its hydrolysates have the potential for preparing functional protein beverages with a shelf-life of up to 7 days.
ISSN:0032-5791