SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing
The COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools for detecting SARS-CoV-2. In this study, we developed and optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity and specificity. Key p...
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MDPI AG
2025-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/21 |
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| author | Talia Tene Stefano Bellucci Cristian Vacacela Gomez |
| author_facet | Talia Tene Stefano Bellucci Cristian Vacacela Gomez |
| author_sort | Talia Tene |
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| description | The COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools for detecting SARS-CoV-2. In this study, we developed and optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity and specificity. Key parameters, including the thickness of the silver layer, silicon nitride dielectric layer, molybdenum disulfide (MoS<sub>2</sub>) layers, and ssDNA recognition layer, were systematically optimized to achieve the best balance between sensitivity, resolution, and attenuation. The optimized configuration, consisting of a 45 nm silver layer, a 13 nm silicon nitride layer, 2 MoS<sub>2</sub> layers, and a 5 nm ssDNA layer, demonstrated superior performance for detecting SARS-CoV-2 in PBS solution. The biosensor exhibited high sensitivity at low viral concentrations, achieving a sensitivity of 375.01°/RIU, a detection accuracy of 0.002, and a quality factor of 38.34 at 1.0 mM SARS-CoV-2 concentration. Performance metrics validated the sensor’s capability for reliable detection, particularly in early-stage diagnostics where timely intervention is critical. Moreover, the biosensor’s linear response to refractive index changes confirmed its potential for quantitative viral concentration analysis. This study underlines the significance of integrating advanced materials, such as MoS<sub>2</sub> and silicon nitride, to enhance SPR biosensor performance. The findings establish the proposed biosensor as a robust and precise diagnostic tool for SARS-CoV-2 detection, with potential applications in clinical diagnostics and epidemiological monitoring. |
| format | Article |
| id | doaj-art-9d6b4211796547358a60f2b3c520e9bc |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj-art-9d6b4211796547358a60f2b3c520e9bc2025-01-24T13:25:27ZengMDPI AGBiosensors2079-63742025-01-011512110.3390/bios15010021SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 SensingTalia Tene0Stefano Bellucci1Cristian Vacacela Gomez2Department of Chemistry, Universidad Técnica Particular de Loja, Loja 110160, EcuadorINFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044 Frascati, ItalyINFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044 Frascati, ItalyThe COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools for detecting SARS-CoV-2. In this study, we developed and optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity and specificity. Key parameters, including the thickness of the silver layer, silicon nitride dielectric layer, molybdenum disulfide (MoS<sub>2</sub>) layers, and ssDNA recognition layer, were systematically optimized to achieve the best balance between sensitivity, resolution, and attenuation. The optimized configuration, consisting of a 45 nm silver layer, a 13 nm silicon nitride layer, 2 MoS<sub>2</sub> layers, and a 5 nm ssDNA layer, demonstrated superior performance for detecting SARS-CoV-2 in PBS solution. The biosensor exhibited high sensitivity at low viral concentrations, achieving a sensitivity of 375.01°/RIU, a detection accuracy of 0.002, and a quality factor of 38.34 at 1.0 mM SARS-CoV-2 concentration. Performance metrics validated the sensor’s capability for reliable detection, particularly in early-stage diagnostics where timely intervention is critical. Moreover, the biosensor’s linear response to refractive index changes confirmed its potential for quantitative viral concentration analysis. This study underlines the significance of integrating advanced materials, such as MoS<sub>2</sub> and silicon nitride, to enhance SPR biosensor performance. The findings establish the proposed biosensor as a robust and precise diagnostic tool for SARS-CoV-2 detection, with potential applications in clinical diagnostics and epidemiological monitoring.https://www.mdpi.com/2079-6374/15/1/21surface plasmon resonanceMoS<sub>2</sub>silicon nitrideSARS-CoV-2biosensor |
| spellingShingle | Talia Tene Stefano Bellucci Cristian Vacacela Gomez SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing Biosensors surface plasmon resonance MoS<sub>2</sub> silicon nitride SARS-CoV-2 biosensor |
| title | SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing |
| title_full | SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing |
| title_fullStr | SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing |
| title_full_unstemmed | SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing |
| title_short | SPR Biosensor Based on Bilayer MoS<sub>2</sub> for SARS-CoV-2 Sensing |
| title_sort | spr biosensor based on bilayer mos sub 2 sub for sars cov 2 sensing |
| topic | surface plasmon resonance MoS<sub>2</sub> silicon nitride SARS-CoV-2 biosensor |
| url | https://www.mdpi.com/2079-6374/15/1/21 |
| work_keys_str_mv | AT taliatene sprbiosensorbasedonbilayermossub2subforsarscov2sensing AT stefanobellucci sprbiosensorbasedonbilayermossub2subforsarscov2sensing AT cristianvacacelagomez sprbiosensorbasedonbilayermossub2subforsarscov2sensing |