Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production
Foxp3+CD25+CD4+ regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype and monitored the migration and activity of regulatory T cells present in the airways of allergic or tolerant mice after allergen challenge. To indu...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/721817 |
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| author | Lucas Faustino Daniel Mucida Alexandre Castro Keller Jocelyne Demengeot Karina Bortoluci Luiz Roberto Sardinha Maisa Carla Takenaka Alexandre Salgado Basso Ana Maria Caetano Faria Momtchilo Russo |
| author_facet | Lucas Faustino Daniel Mucida Alexandre Castro Keller Jocelyne Demengeot Karina Bortoluci Luiz Roberto Sardinha Maisa Carla Takenaka Alexandre Salgado Basso Ana Maria Caetano Faria Momtchilo Russo |
| author_sort | Lucas Faustino |
| collection | DOAJ |
| description | Foxp3+CD25+CD4+ regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype and monitored the migration and activity of regulatory T cells present in the airways of allergic or tolerant mice after allergen challenge. To induce lung allergic inflammation, mice were sensitized twice with ovalbumin/aluminum hydroxide gel and challenged twice with intranasal ovalbumin. Tolerance was induced by oral administration of ovalbumin for 5 consecutive days prior to OVA sensitization and challenge. We detected regulatory T cells (Foxp3+CD25+CD4+ T cells) in the airways of allergic and tolerant mice; however, the number of regulatory T cells was more than 40-fold higher in allergic mice than in tolerant mice. Lung regulatory T cells expressed an effector/memory phenotype (CCR4highCD62LlowCD44highCD54highCD69+) that distinguished them from naive regulatory T cells (CCR4intCD62LhighCD44intCD54intCD69−). These regulatory T cells efficiently suppressed pulmonary T-cell proliferation but not Th2 cytokine production. |
| format | Article |
| id | doaj-art-9d52e72f3b6845499737e7643292b3c1 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-9d52e72f3b6845499737e7643292b3c12025-02-03T06:11:21ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/721817721817Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine ProductionLucas Faustino0Daniel Mucida1Alexandre Castro Keller2Jocelyne Demengeot3Karina Bortoluci4Luiz Roberto Sardinha5Maisa Carla Takenaka6Alexandre Salgado Basso7Ana Maria Caetano Faria8Momtchilo Russo9Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-000 São Paulo, SP, BrazilLaboratory of Mucosal Immunology, The Rockefeller University, New York, NY 10065, USADepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, 04023-900 São Paulo, SP, BrazilInstituto Gulbenkian de Ciência, 2780-901 Oeiras, PortugalDepartamento de Ciências Biológicas, Campus Diadema e Centro de Terapia Celular e Molecular (CTCMol), Universidade Federal de São Paulo, 04044-010 São Paulo, SP, BrazilInstituto Israelita de Ensino e Pesquisa Albert Einstein, 05652-900 São Paulo, SP, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, 04023-900 São Paulo, SP, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, 04023-900 São Paulo, SP, BrazilDepartamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-000 São Paulo, SP, BrazilFoxp3+CD25+CD4+ regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype and monitored the migration and activity of regulatory T cells present in the airways of allergic or tolerant mice after allergen challenge. To induce lung allergic inflammation, mice were sensitized twice with ovalbumin/aluminum hydroxide gel and challenged twice with intranasal ovalbumin. Tolerance was induced by oral administration of ovalbumin for 5 consecutive days prior to OVA sensitization and challenge. We detected regulatory T cells (Foxp3+CD25+CD4+ T cells) in the airways of allergic and tolerant mice; however, the number of regulatory T cells was more than 40-fold higher in allergic mice than in tolerant mice. Lung regulatory T cells expressed an effector/memory phenotype (CCR4highCD62LlowCD44highCD54highCD69+) that distinguished them from naive regulatory T cells (CCR4intCD62LhighCD44intCD54intCD69−). These regulatory T cells efficiently suppressed pulmonary T-cell proliferation but not Th2 cytokine production.http://dx.doi.org/10.1155/2012/721817 |
| spellingShingle | Lucas Faustino Daniel Mucida Alexandre Castro Keller Jocelyne Demengeot Karina Bortoluci Luiz Roberto Sardinha Maisa Carla Takenaka Alexandre Salgado Basso Ana Maria Caetano Faria Momtchilo Russo Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production Clinical and Developmental Immunology |
| title | Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production |
| title_full | Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production |
| title_fullStr | Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production |
| title_full_unstemmed | Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production |
| title_short | Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production |
| title_sort | regulatory t cells accumulate in the lung allergic inflammation and efficiently suppress t cell proliferation but not th2 cytokine production |
| url | http://dx.doi.org/10.1155/2012/721817 |
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