Decreased Expression of GPER1 Gene and Protein in Goiter
Goiter is more common in women, suggesting that estrogen could be involved in its physiopathology. The presence of classical estrogen receptors (ERα and ERβ) has been described in thyroid tissue, suggesting a direct effect of estrogen on the gland. A nonclassic estrogen receptor, the G-protein-coupl...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/869431 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562624590512128 |
|---|---|
| author | Raquel Weber Ana Paula Santin Bertoni Laura Walter Bessestil Ilma Simoni Brum Tania Weber Furlanetto |
| author_facet | Raquel Weber Ana Paula Santin Bertoni Laura Walter Bessestil Ilma Simoni Brum Tania Weber Furlanetto |
| author_sort | Raquel Weber |
| collection | DOAJ |
| description | Goiter is more common in women, suggesting that estrogen could be involved in its physiopathology. The presence of classical estrogen receptors (ERα and ERβ) has been described in thyroid tissue, suggesting a direct effect of estrogen on the gland. A nonclassic estrogen receptor, the G-protein-coupled estrogen receptor (GPER1), has been described recently in several tissues. However, in goiter, the presence of this receptor has not been studied yet. We investigated GPER1 gene and protein expressions in normal thyroid and goiter using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. In normal thyroid (n=16) and goiter (n=19), GPER1 gene was expressed in all samples, while GPER1 protein was expressed in all samples of normal thyroid (n=15) but in only 72% of goiter samples (n=13). When comparing GPER1 gene and protein levels in both conditions, gene expression and protein levels were higher in normal thyroid than in goiter, suggesting a role of this receptor in this condition. Further studies are needed to elucidate the role of GPER1 in normal thyroid and goiter. |
| format | Article |
| id | doaj-art-9d3502c03efc409c95141556669b8e78 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-9d3502c03efc409c95141556669b8e782025-02-03T01:22:11ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/869431869431Decreased Expression of GPER1 Gene and Protein in GoiterRaquel Weber0Ana Paula Santin Bertoni1Laura Walter Bessestil2Ilma Simoni Brum3Tania Weber Furlanetto4Programa de Pós Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2400, 90035-903 Porto Alegre, RS, BrazilPrograma de Pós Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite 245, 90050-170 Porto Alegre, RS, BrazilDepartamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, 90050-170 Porto Alegre, RS, BrazilDepartamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, 90050-170 Porto Alegre, RS, BrazilPrograma de Pós Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2400, 90035-903 Porto Alegre, RS, BrazilGoiter is more common in women, suggesting that estrogen could be involved in its physiopathology. The presence of classical estrogen receptors (ERα and ERβ) has been described in thyroid tissue, suggesting a direct effect of estrogen on the gland. A nonclassic estrogen receptor, the G-protein-coupled estrogen receptor (GPER1), has been described recently in several tissues. However, in goiter, the presence of this receptor has not been studied yet. We investigated GPER1 gene and protein expressions in normal thyroid and goiter using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. In normal thyroid (n=16) and goiter (n=19), GPER1 gene was expressed in all samples, while GPER1 protein was expressed in all samples of normal thyroid (n=15) but in only 72% of goiter samples (n=13). When comparing GPER1 gene and protein levels in both conditions, gene expression and protein levels were higher in normal thyroid than in goiter, suggesting a role of this receptor in this condition. Further studies are needed to elucidate the role of GPER1 in normal thyroid and goiter.http://dx.doi.org/10.1155/2015/869431 |
| spellingShingle | Raquel Weber Ana Paula Santin Bertoni Laura Walter Bessestil Ilma Simoni Brum Tania Weber Furlanetto Decreased Expression of GPER1 Gene and Protein in Goiter International Journal of Endocrinology |
| title | Decreased Expression of GPER1 Gene and Protein in Goiter |
| title_full | Decreased Expression of GPER1 Gene and Protein in Goiter |
| title_fullStr | Decreased Expression of GPER1 Gene and Protein in Goiter |
| title_full_unstemmed | Decreased Expression of GPER1 Gene and Protein in Goiter |
| title_short | Decreased Expression of GPER1 Gene and Protein in Goiter |
| title_sort | decreased expression of gper1 gene and protein in goiter |
| url | http://dx.doi.org/10.1155/2015/869431 |
| work_keys_str_mv | AT raquelweber decreasedexpressionofgper1geneandproteiningoiter AT anapaulasantinbertoni decreasedexpressionofgper1geneandproteiningoiter AT laurawalterbessestil decreasedexpressionofgper1geneandproteiningoiter AT ilmasimonibrum decreasedexpressionofgper1geneandproteiningoiter AT taniaweberfurlanetto decreasedexpressionofgper1geneandproteiningoiter |