Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits
In this study, cerium dioxide-modified cobalt oxyhydroxide (CeO2/CoOOH) nanomaterial was synthesized by a liquid-phase precipitation method. CeO2/CoOOH showed good oxidase-like activity toward 3,3’,5,5’-tetramethylbenzidine (TMB), and could directly oxidize TMB to ox-TMB, which presented a clear blu...
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China Food Publishing Company
2024-12-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-028.pdf |
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| author | YUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo |
| author_facet | YUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo |
| author_sort | YUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo |
| collection | DOAJ |
| description | In this study, cerium dioxide-modified cobalt oxyhydroxide (CeO2/CoOOH) nanomaterial was synthesized by a liquid-phase precipitation method. CeO2/CoOOH showed good oxidase-like activity toward 3,3’,5,5’-tetramethylbenzidine (TMB), and could directly oxidize TMB to ox-TMB, which presented a clear blue color. Reversely, ascorbic acid (AA) could reduce ox-TMB to TMB, leading to a decrease in the absorbance of ox-TMB at 652 nm and a fading of the blue color. Based on this phenomenon, a new colorimetric sensor for AA detection was developed. The sensor exhibited a highly sensitive response to AA with a limit of detection (LOD) of 2.73 μg/mL and a wide detection range of 5-100 μg/mL and could be successfully applied for rapid detection of AA in oranges and apples. |
| format | Article |
| id | doaj-art-9cdf8851849e44158b43e9d37cb5aed2 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-9cdf8851849e44158b43e9d37cb5aed22025-02-05T09:08:01ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452425025810.7506/spkx1002-6630-20240510-074Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in FruitsYUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo0(1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; 2. Key Laboratory of Cold Chain Food Processing and Safety Control, Ministry of Education, Zhengzhou 450001, China; 3. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China)In this study, cerium dioxide-modified cobalt oxyhydroxide (CeO2/CoOOH) nanomaterial was synthesized by a liquid-phase precipitation method. CeO2/CoOOH showed good oxidase-like activity toward 3,3’,5,5’-tetramethylbenzidine (TMB), and could directly oxidize TMB to ox-TMB, which presented a clear blue color. Reversely, ascorbic acid (AA) could reduce ox-TMB to TMB, leading to a decrease in the absorbance of ox-TMB at 652 nm and a fading of the blue color. Based on this phenomenon, a new colorimetric sensor for AA detection was developed. The sensor exhibited a highly sensitive response to AA with a limit of detection (LOD) of 2.73 μg/mL and a wide detection range of 5-100 μg/mL and could be successfully applied for rapid detection of AA in oranges and apples.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-028.pdfrapid detection; colorimetric method; ascorbic acid; nanozyme |
| spellingShingle | YUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits Shipin Kexue rapid detection; colorimetric method; ascorbic acid; nanozyme |
| title | Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits |
| title_full | Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits |
| title_fullStr | Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits |
| title_full_unstemmed | Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits |
| title_short | Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits |
| title_sort | colorimetric method based on ceo2 coooh nanozyme for rapid detection of ascorbic acid in fruits |
| topic | rapid detection; colorimetric method; ascorbic acid; nanozyme |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-028.pdf |
| work_keys_str_mv | AT yuexiaoyuewuchaoyunyanshuliangfulongzhaodianbo colorimetricmethodbasedonceo2cooohnanozymeforrapiddetectionofascorbicacidinfruits |