Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors
Objective: To assess the protective effects of trigonelline against spinal cord injury (SCI) in rats. Methods: Rats (Sprague-Dawley, male) were randomly assigned to seven groups (n=15 per group): normal, sham, SCI control (1% DMSO), methylprednisolone (30 mg/kg), and trigonelline (50, 100, and 200 m...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
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| Online Access: | https://journals.lww.com/10.4103/apjtb.apjtb_519_24 |
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| author | Zhi-Lan Ye Yuan Cao |
| author_facet | Zhi-Lan Ye Yuan Cao |
| author_sort | Zhi-Lan Ye |
| collection | DOAJ |
| description | Objective:
To assess the protective effects of trigonelline against spinal cord injury (SCI) in rats.
Methods:
Rats (Sprague-Dawley, male) were randomly assigned to seven groups (n=15 per group): normal, sham, SCI control (1% DMSO), methylprednisolone (30 mg/kg), and trigonelline (50, 100, and 200 mg/kg). Rats received respective treatment daily for 28 days. SCI was induced by using a temporary aneurysm clip. Behavioral, biochemical, and histological analyses were performed to investigate the neuroprotective effect of trigonelline.
Results:
Trigonelline (100 and 200 mg/kg) treatment effectively (P<0.05) mitigated SCI-induced changes in mechano-tactile sensation, allodynia, hyperalgesia, and motor nerve conduction velocity. It notably (P<0.05) downregulated apoptotic (Bax and caspase-3) and inflammatory (COX-II) markers, while upregulating Bcl-2 and BDNF mRNA expression in the spinal cord (P<0.05). Furthermore, trigonelline effectively alleviated (P<0.05) SCI-induced alterations in mitochondrial complex levels, resulting in enhanced nicotinamide adenine dinucleotide dehydrogenase, succinate dehydrogenase, redox activity, and cytochrome-C levels. Histological examination of spinal cord tissue indicated that trigonelline significantly (P<0.05) ameliorated the histological damage caused by SCI, thereby improving neuronal degeneration, inflammatory cell infiltration, and necrosis.
Conclusions:
Trigonelline shows neuroprotective properties in SCI rats by reducing allodynia, hyperalgesia, and inflammation, stabilizing mitochondrial enzyme complexes, and modulating apoptotic and neurotrophic factors. Thus, trigonelline holds promise as a potential neuroprotective agent. |
| format | Article |
| id | doaj-art-9cd65f269d284f25b835aa72bfecbd9d |
| institution | Kabale University |
| issn | 2221-1691 2588-9222 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj-art-9cd65f269d284f25b835aa72bfecbd9d2025-02-06T04:54:52ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222025-01-01151344210.4103/apjtb.apjtb_519_24Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factorsZhi-Lan YeYuan CaoObjective: To assess the protective effects of trigonelline against spinal cord injury (SCI) in rats. Methods: Rats (Sprague-Dawley, male) were randomly assigned to seven groups (n=15 per group): normal, sham, SCI control (1% DMSO), methylprednisolone (30 mg/kg), and trigonelline (50, 100, and 200 mg/kg). Rats received respective treatment daily for 28 days. SCI was induced by using a temporary aneurysm clip. Behavioral, biochemical, and histological analyses were performed to investigate the neuroprotective effect of trigonelline. Results: Trigonelline (100 and 200 mg/kg) treatment effectively (P<0.05) mitigated SCI-induced changes in mechano-tactile sensation, allodynia, hyperalgesia, and motor nerve conduction velocity. It notably (P<0.05) downregulated apoptotic (Bax and caspase-3) and inflammatory (COX-II) markers, while upregulating Bcl-2 and BDNF mRNA expression in the spinal cord (P<0.05). Furthermore, trigonelline effectively alleviated (P<0.05) SCI-induced alterations in mitochondrial complex levels, resulting in enhanced nicotinamide adenine dinucleotide dehydrogenase, succinate dehydrogenase, redox activity, and cytochrome-C levels. Histological examination of spinal cord tissue indicated that trigonelline significantly (P<0.05) ameliorated the histological damage caused by SCI, thereby improving neuronal degeneration, inflammatory cell infiltration, and necrosis. Conclusions: Trigonelline shows neuroprotective properties in SCI rats by reducing allodynia, hyperalgesia, and inflammation, stabilizing mitochondrial enzyme complexes, and modulating apoptotic and neurotrophic factors. Thus, trigonelline holds promise as a potential neuroprotective agent.https://journals.lww.com/10.4103/apjtb.apjtb_519_24baxbcl-2bdnfcox-iispinal cord injurytrigonelline |
| spellingShingle | Zhi-Lan Ye Yuan Cao Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors Asian Pacific Journal of Tropical Biomedicine bax bcl-2 bdnf cox-ii spinal cord injury trigonelline |
| title | Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors |
| title_full | Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors |
| title_fullStr | Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors |
| title_full_unstemmed | Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors |
| title_short | Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors |
| title_sort | trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation apoptosis and neurotrophic factors |
| topic | bax bcl-2 bdnf cox-ii spinal cord injury trigonelline |
| url | https://journals.lww.com/10.4103/apjtb.apjtb_519_24 |
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