Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet
With the increasing annual production of Lentinula edodes, the residues of Lentinus edodes are mass produced and wasted every year. In order to further explore the added value and effective utilization of Lentinus edodes, we studied the lipid-lowering efficacy and liver protective effect of Lentinus...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1537569/full |
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| author | Kangxiao Guo Kangxiao Guo Jing Liu Zihan Yao Zhoujin Tan Tao Yang |
| author_facet | Kangxiao Guo Kangxiao Guo Jing Liu Zihan Yao Zhoujin Tan Tao Yang |
| author_sort | Kangxiao Guo |
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| description | With the increasing annual production of Lentinula edodes, the residues of Lentinus edodes are mass produced and wasted every year. In order to further explore the added value and effective utilization of Lentinus edodes, we studied the lipid-lowering efficacy and liver protective effect of Lentinus edodes soluble dietary fiber in mice on high-fat diet. Project team from Lentinus edodes extracted soluble dietary fiber, and its physicochemical properties, selected 30 male mice, randomly divided into normal group (N), high fat diet group (F), add low dose dietary fiber high fat diet (FL), add medium dose dietary fiber high fat diet group (FM), add high dose dietary fiber high fat diet group (FH) five groups. After 4 weeks, we assessed general state, organ conditions, liver status, blood parameters, expression of hepatic lipid metabolism genes, mRNA levels of key hepatic lipid metabolism genes. The results showed that the molecular weight of soluble dietary fiber is about 17.029 kDa, and the monosaccharides such as galactose, glucose and mannitol are connected by β-glycosidic bond. The soluble dietary fiber of Lentinus edodes can effectively slow the weight growth due to high-fat diet, delay liver tissue lesions, reduce the levels of ALT, AST, ACP, LDL-C, TG, TV, FFA, SOD, GSH and MDA, and increase the levels of γ-GT, HDL-C and CAT in blood. Lentinus edodes soluble dietary fiber decreased the expression of AMPKα and SREBP-2 in the liver, and increased the expression of PPARα, ACS, CPT1a, CYP7A1. It is proved that the soluble dietary fiber of Lentinus edodes can alleviate the organ fat accumulation caused by high-fat diet to some extent, effectively combat the liver injury, oxidative stress pressure and lipid metabolism disorder caused by high-fat diet, and provide an experimental basis for the subsequent effective use of soluble dietary fiber of Lentinus edodes in fat reduction. |
| format | Article |
| id | doaj-art-9cd5c278d1304c72ac5153448d90d605 |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj-art-9cd5c278d1304c72ac5153448d90d6052025-01-30T05:10:07ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-01-011210.3389/fnut.2025.15375691537569Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat dietKangxiao Guo0Kangxiao Guo1Jing Liu2Zihan Yao3Zhoujin Tan4Tao Yang5National Engineering Laboratory for Rice and By-Product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaDepartment of Pharmacy, Changsha Health Vocational College, Changsha, ChinaCollege of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaNational Engineering Laboratory for Rice and By-Product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaCollege of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaNational Engineering Laboratory for Rice and By-Product Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaWith the increasing annual production of Lentinula edodes, the residues of Lentinus edodes are mass produced and wasted every year. In order to further explore the added value and effective utilization of Lentinus edodes, we studied the lipid-lowering efficacy and liver protective effect of Lentinus edodes soluble dietary fiber in mice on high-fat diet. Project team from Lentinus edodes extracted soluble dietary fiber, and its physicochemical properties, selected 30 male mice, randomly divided into normal group (N), high fat diet group (F), add low dose dietary fiber high fat diet (FL), add medium dose dietary fiber high fat diet group (FM), add high dose dietary fiber high fat diet group (FH) five groups. After 4 weeks, we assessed general state, organ conditions, liver status, blood parameters, expression of hepatic lipid metabolism genes, mRNA levels of key hepatic lipid metabolism genes. The results showed that the molecular weight of soluble dietary fiber is about 17.029 kDa, and the monosaccharides such as galactose, glucose and mannitol are connected by β-glycosidic bond. The soluble dietary fiber of Lentinus edodes can effectively slow the weight growth due to high-fat diet, delay liver tissue lesions, reduce the levels of ALT, AST, ACP, LDL-C, TG, TV, FFA, SOD, GSH and MDA, and increase the levels of γ-GT, HDL-C and CAT in blood. Lentinus edodes soluble dietary fiber decreased the expression of AMPKα and SREBP-2 in the liver, and increased the expression of PPARα, ACS, CPT1a, CYP7A1. It is proved that the soluble dietary fiber of Lentinus edodes can alleviate the organ fat accumulation caused by high-fat diet to some extent, effectively combat the liver injury, oxidative stress pressure and lipid metabolism disorder caused by high-fat diet, and provide an experimental basis for the subsequent effective use of soluble dietary fiber of Lentinus edodes in fat reduction.https://www.frontiersin.org/articles/10.3389/fnut.2025.1537569/fullLentinusedodessoluble dietary fiberlipid metabolismliver protectionhigh fat diet |
| spellingShingle | Kangxiao Guo Kangxiao Guo Jing Liu Zihan Yao Zhoujin Tan Tao Yang Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet Frontiers in Nutrition Lentinusedodes soluble dietary fiber lipid metabolism liver protection high fat diet |
| title | Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet |
| title_full | Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet |
| title_fullStr | Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet |
| title_full_unstemmed | Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet |
| title_short | Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet |
| title_sort | effect of soluble dietary fiber extracted from lentinula edodes berk pegler on lipid metabolism and liver protection in mice on high fat diet |
| topic | Lentinusedodes soluble dietary fiber lipid metabolism liver protection high fat diet |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1537569/full |
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