Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2
Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of micro...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2022-09-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3433 |
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| author | K. Yu. Kargapolova O. V. Tkachenko G. I. Burygin N. V. Evseeva A. A. Shirokov L. Yu. Matora S. Yu. Shchyogolev |
| author_facet | K. Yu. Kargapolova O. V. Tkachenko G. I. Burygin N. V. Evseeva A. A. Shirokov L. Yu. Matora S. Yu. Shchyogolev |
| author_sort | K. Yu. Kargapolova |
| collection | DOAJ |
| description | Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material. |
| format | Article |
| id | doaj-art-9cca546198214af7ab708d4ca9f2542e |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-9cca546198214af7ab708d4ca9f2542e2025-02-01T09:58:11ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592022-09-0126542243010.18699/VJGB-22-521276Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2K. Yu. Kargapolova0O. V. Tkachenko1G. I. Burygin2N. V. Evseeva3A. A. Shirokov4L. Yu. Matora5S. Yu. Shchyogolev6Saratov State Vavilov Agrarian UniversitySaratov State Vavilov Agrarian UniversitySaratov State Vavilov Agrarian University; Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesInstitute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesInstitute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesInstitute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesInstitute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Saratov Federal Scientific Centre of the Russian Academy of SciencesSustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material.https://vavilov.elpub.ru/jour/article/view/3433<i>solanum tuberosum</i> l.<i>azospirillum baldaniorum</i> sp245<i>ochrobactrum cytisi</i> ipa7.2plant-microbe associations, clonal micropropagationplant growth efficacyadaptability<i>in vitro</i><i>ex vitro</i> |
| spellingShingle | K. Yu. Kargapolova O. V. Tkachenko G. I. Burygin N. V. Evseeva A. A. Shirokov L. Yu. Matora S. Yu. Shchyogolev Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2 Вавиловский журнал генетики и селекции <i>solanum tuberosum</i> l. <i>azospirillum baldaniorum</i> sp245 <i>ochrobactrum cytisi</i> ipa7.2 plant-microbe associations, clonal micropropagation plant growth efficacy adaptability <i>in vitro</i> <i>ex vitro</i> |
| title | Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2 |
| title_full | Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2 |
| title_fullStr | Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2 |
| title_full_unstemmed | Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2 |
| title_short | Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2 |
| title_sort | improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria i azospirillum baldaniorum i sp245 and i ochrobactrum cytisi i ipa7 2 |
| topic | <i>solanum tuberosum</i> l. <i>azospirillum baldaniorum</i> sp245 <i>ochrobactrum cytisi</i> ipa7.2 plant-microbe associations, clonal micropropagation plant growth efficacy adaptability <i>in vitro</i> <i>ex vitro</i> |
| url | https://vavilov.elpub.ru/jour/article/view/3433 |
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