Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells
Cell origami has been widely used in the field of three-dimensional (3D) cell-populated microstructures due to their multiple advantages, including high biocompatibility, the lack of special requirements for substrate materials, and the lack of damage to cells. A 3D finite element method (FEM) model...
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Wiley
2019-01-01
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| Series: | Applied Bionics and Biomechanics |
| Online Access: | http://dx.doi.org/10.1155/2019/8541303 |
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| author | Lili Wang Weiyi Chen |
| author_facet | Lili Wang Weiyi Chen |
| author_sort | Lili Wang |
| collection | DOAJ |
| description | Cell origami has been widely used in the field of three-dimensional (3D) cell-populated microstructures due to their multiple advantages, including high biocompatibility, the lack of special requirements for substrate materials, and the lack of damage to cells. A 3D finite element method (FEM) model of an adherent cell based on the tensegrity structure is constructed to describe cell origami by using the principle of the origami folding technique and cell traction forces. Adherent cell models contain a cytoskeleton (CSK), which is primarily composed of microtubules (MTs), microfilaments (MFs), intermediate filaments (IFs), and a nucleoskeleton (NSK), which is mainly made up of the nuclear lamina and chromatin. The microplate is assumed to be an isotropic linear-elastic solid material with a flexible joint that is connected to the cell tensegrity structure model by spring elements representing focal adhesion complexes (FACs). To investigate the effects of the degree of complexity of the tensegrity structure and NSK on the folding angle of the microplate, four models are established in the study. The results demonstrate that the inclusion of the NSK can increase the folding angle of the microplate, indicating that the cell is closer to its physiological environment, while increased complexity can reduce the folding angle of the microplate since the folding angle is depended on the cell types. The proposed adherent cell FEM models are validated by comparisons with reported results. These findings can provide theoretical guidance for the application of biotechnology and the analysis of 3D structures of cells and have profound implications for the self-assembly of cell-based microscale medical devices. |
| format | Article |
| id | doaj-art-9c9b65fdf9b04905820e24b49fed94b6 |
| institution | Kabale University |
| issn | 1176-2322 1754-2103 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied Bionics and Biomechanics |
| spelling | doaj-art-9c9b65fdf9b04905820e24b49fed94b62025-02-03T01:26:35ZengWileyApplied Bionics and Biomechanics1176-23221754-21032019-01-01201910.1155/2019/85413038541303Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent CellsLili Wang0Weiyi Chen1Shanxi Key Laboratory of Material Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, ChinaShanxi Key Laboratory of Material Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, ChinaCell origami has been widely used in the field of three-dimensional (3D) cell-populated microstructures due to their multiple advantages, including high biocompatibility, the lack of special requirements for substrate materials, and the lack of damage to cells. A 3D finite element method (FEM) model of an adherent cell based on the tensegrity structure is constructed to describe cell origami by using the principle of the origami folding technique and cell traction forces. Adherent cell models contain a cytoskeleton (CSK), which is primarily composed of microtubules (MTs), microfilaments (MFs), intermediate filaments (IFs), and a nucleoskeleton (NSK), which is mainly made up of the nuclear lamina and chromatin. The microplate is assumed to be an isotropic linear-elastic solid material with a flexible joint that is connected to the cell tensegrity structure model by spring elements representing focal adhesion complexes (FACs). To investigate the effects of the degree of complexity of the tensegrity structure and NSK on the folding angle of the microplate, four models are established in the study. The results demonstrate that the inclusion of the NSK can increase the folding angle of the microplate, indicating that the cell is closer to its physiological environment, while increased complexity can reduce the folding angle of the microplate since the folding angle is depended on the cell types. The proposed adherent cell FEM models are validated by comparisons with reported results. These findings can provide theoretical guidance for the application of biotechnology and the analysis of 3D structures of cells and have profound implications for the self-assembly of cell-based microscale medical devices.http://dx.doi.org/10.1155/2019/8541303 |
| spellingShingle | Lili Wang Weiyi Chen Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells Applied Bionics and Biomechanics |
| title | Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells |
| title_full | Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells |
| title_fullStr | Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells |
| title_full_unstemmed | Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells |
| title_short | Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells |
| title_sort | modelling cell origami via a tensegrity model of the cytoskeleton in adherent cells |
| url | http://dx.doi.org/10.1155/2019/8541303 |
| work_keys_str_mv | AT liliwang modellingcellorigamiviaatensegritymodelofthecytoskeletoninadherentcells AT weiyichen modellingcellorigamiviaatensegritymodelofthecytoskeletoninadherentcells |