A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii)
Abstract Fat greenling (Hexagrammos otakii Jordan & Starks, 1895) is a valuable marine fish species, crucial for aquaculture in Northern China due to its high-quality meat and significant economic value. However, the aquaculture industry faces challenges such as trait degradation, early sexual m...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04368-3 |
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| author | Li Li Fawen Hu Dong Liu Xiaolong Wang Jing Diao Yijing Zhu Fengxiang Gao Ying Fan Yuxia Jian Xue Wang Lei Pan Wen Guo |
| author_facet | Li Li Fawen Hu Dong Liu Xiaolong Wang Jing Diao Yijing Zhu Fengxiang Gao Ying Fan Yuxia Jian Xue Wang Lei Pan Wen Guo |
| author_sort | Li Li |
| collection | DOAJ |
| description | Abstract Fat greenling (Hexagrammos otakii Jordan & Starks, 1895) is a valuable marine fish species, crucial for aquaculture in Northern China due to its high-quality meat and significant economic value. However, the aquaculture industry faces challenges such as trait degradation, early sexual maturity, and disease susceptibility, necessitating advanced genomic interventions for sustainable cultivation. This study presents the first chromosomal-level genome assembly of H. otakii, achieved using PacBio long-read sequencing and Hi-C technology. The assembly yielded a genome size of 682.43 Mb with a contig N50 size of 2.39 Mb and a scaffold N50 size of 27.83 Mb. The completeness of genome assessed by BUSCO is 96.99%. A total of 22,334 protein-coding genes were predicted, with 21,619 (96.80%) functionally annotated across various protein databases. This genomic resource is a step forward in supporting the breeding, germplasm conservation, and enhancement of H. otakii, facilitating genetic studies and the development of strategies for disease resistance and growth optimization in aquaculture. |
| format | Article |
| id | doaj-art-9c886be0dc214bebbf152a9a411d4fee |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-9c886be0dc214bebbf152a9a411d4fee2025-01-19T12:09:43ZengNature PortfolioScientific Data2052-44632025-01-0112111010.1038/s41597-025-04368-3A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii)Li Li0Fawen Hu1Dong Liu2Xiaolong Wang3Jing Diao4Yijing Zhu5Fengxiang Gao6Ying Fan7Yuxia Jian8Xue Wang9Lei Pan10Wen Guo11Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Shandong Key Laboratory of Disease Control in Mariculture, Key Laboratory of Benthic Fisheries Aquaculture and Enhancement, Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao)Abstract Fat greenling (Hexagrammos otakii Jordan & Starks, 1895) is a valuable marine fish species, crucial for aquaculture in Northern China due to its high-quality meat and significant economic value. However, the aquaculture industry faces challenges such as trait degradation, early sexual maturity, and disease susceptibility, necessitating advanced genomic interventions for sustainable cultivation. This study presents the first chromosomal-level genome assembly of H. otakii, achieved using PacBio long-read sequencing and Hi-C technology. The assembly yielded a genome size of 682.43 Mb with a contig N50 size of 2.39 Mb and a scaffold N50 size of 27.83 Mb. The completeness of genome assessed by BUSCO is 96.99%. A total of 22,334 protein-coding genes were predicted, with 21,619 (96.80%) functionally annotated across various protein databases. This genomic resource is a step forward in supporting the breeding, germplasm conservation, and enhancement of H. otakii, facilitating genetic studies and the development of strategies for disease resistance and growth optimization in aquaculture.https://doi.org/10.1038/s41597-025-04368-3 |
| spellingShingle | Li Li Fawen Hu Dong Liu Xiaolong Wang Jing Diao Yijing Zhu Fengxiang Gao Ying Fan Yuxia Jian Xue Wang Lei Pan Wen Guo A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii) Scientific Data |
| title | A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii) |
| title_full | A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii) |
| title_fullStr | A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii) |
| title_full_unstemmed | A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii) |
| title_short | A Chromosomal-level genome assembly and annotation of fat greenling (Hexagrammos otakii) |
| title_sort | chromosomal level genome assembly and annotation of fat greenling hexagrammos otakii |
| url | https://doi.org/10.1038/s41597-025-04368-3 |
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