High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim
Triflumezopyrim, a newly commercialized insecticide that belongs to a novel class of mesoionic insecticides, is widely used in rice fields for controlling brown planthoppers. Insecticides infiltrate from crop fields to aquatic ecosystems through leaching and rainwater, thereby affecting aquatic orga...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000377 |
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| author | Ipsita Iswari Das Bayasis M. Sharma Madhusmita Mahapatra Sipra Nayak Aman Kumar Mohanty Lakshman Sahoo Rajesh Kumar Jitendra Kumar Sundaray |
| author_facet | Ipsita Iswari Das Bayasis M. Sharma Madhusmita Mahapatra Sipra Nayak Aman Kumar Mohanty Lakshman Sahoo Rajesh Kumar Jitendra Kumar Sundaray |
| author_sort | Ipsita Iswari Das |
| collection | DOAJ |
| description | Triflumezopyrim, a newly commercialized insecticide that belongs to a novel class of mesoionic insecticides, is widely used in rice fields for controlling brown planthoppers. Insecticides infiltrate from crop fields to aquatic ecosystems through leaching and rainwater, thereby affecting aquatic organisms. However, the effects of triflumezopyrim on the gut microbiota of zebrafish (Danio rerio) are largely unknown. Herein, we utilized 16S rRNA gene sequencing to reveal the aquatic eco-risk of triflumezopyrim in the gut microbiota of zebrafish. Fishes were exposed to a sublethal concentration (6.77 ppm) of triflumezopyrim for 21 days. Significant changes in the abundance, diversity, and function of gut microbiota were observed. Specifically, the relative abundance of Proteobacteria and Tenericutes increased in the treatment group. In addition, functional potentials (e.g., metabolism, environmental information processing, and cellular processes) in the gut were significantly affected. The results of this study will contribute to a better understanding of the ecological risk of triflumezopyrim in the gut microbiota of fishes. This is the first sequencing study that provided comprehensive insight into the changes of gut bacterial communities in Danio rerio exposed to triflumezopyrim. |
| format | Article |
| id | doaj-art-9c5f4ecb9b414b3ebfa3e859a329fd33 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-9c5f4ecb9b414b3ebfa3e859a329fd332025-02-06T05:12:26ZengElsevierAquaculture Reports2352-51342025-03-0140102651High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrimIpsita Iswari Das0Bayasis M. Sharma1Madhusmita Mahapatra2Sipra Nayak3Aman Kumar Mohanty4Lakshman Sahoo5Rajesh Kumar6Jitendra Kumar Sundaray7Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaFish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaFish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaFish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaFish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaFish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaAquaculture Production and Environment Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, IndiaFish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha 751002, India; Corresponding author.Triflumezopyrim, a newly commercialized insecticide that belongs to a novel class of mesoionic insecticides, is widely used in rice fields for controlling brown planthoppers. Insecticides infiltrate from crop fields to aquatic ecosystems through leaching and rainwater, thereby affecting aquatic organisms. However, the effects of triflumezopyrim on the gut microbiota of zebrafish (Danio rerio) are largely unknown. Herein, we utilized 16S rRNA gene sequencing to reveal the aquatic eco-risk of triflumezopyrim in the gut microbiota of zebrafish. Fishes were exposed to a sublethal concentration (6.77 ppm) of triflumezopyrim for 21 days. Significant changes in the abundance, diversity, and function of gut microbiota were observed. Specifically, the relative abundance of Proteobacteria and Tenericutes increased in the treatment group. In addition, functional potentials (e.g., metabolism, environmental information processing, and cellular processes) in the gut were significantly affected. The results of this study will contribute to a better understanding of the ecological risk of triflumezopyrim in the gut microbiota of fishes. This is the first sequencing study that provided comprehensive insight into the changes of gut bacterial communities in Danio rerio exposed to triflumezopyrim.http://www.sciencedirect.com/science/article/pii/S2352513425000377Gut microbiotaZebrafishXenobiotic degradationPesticideTriflumezopyrim |
| spellingShingle | Ipsita Iswari Das Bayasis M. Sharma Madhusmita Mahapatra Sipra Nayak Aman Kumar Mohanty Lakshman Sahoo Rajesh Kumar Jitendra Kumar Sundaray High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim Aquaculture Reports Gut microbiota Zebrafish Xenobiotic degradation Pesticide Triflumezopyrim |
| title | High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim |
| title_full | High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim |
| title_fullStr | High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim |
| title_full_unstemmed | High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim |
| title_short | High-throughput sequencing of 16S rRNA gene analysis reveal insights into abundance, diversity, and function of the gut microbiota in zebrafish exposed to triflumezopyrim |
| title_sort | high throughput sequencing of 16s rrna gene analysis reveal insights into abundance diversity and function of the gut microbiota in zebrafish exposed to triflumezopyrim |
| topic | Gut microbiota Zebrafish Xenobiotic degradation Pesticide Triflumezopyrim |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000377 |
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