Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L.
Background. One of the important indicators of the nutritional value of amaranth is the high content of protein and lipids in seeds. Hence, obtaining and identifying such forms of amaranth through breeding, so that they also possessed resistance to abiotic stressors, is an important task.Materials a...
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2022-04-01
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| Series: | Труды по прикладной ботанике, генетике и селекции |
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| Online Access: | https://elpub.vir.nw.ru/jour/article/view/1193 |
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| author | R. M. Taipova V. N. Nesterov O. A. Rozentsvet B. R. Kuluev |
| author_facet | R. M. Taipova V. N. Nesterov O. A. Rozentsvet B. R. Kuluev |
| author_sort | R. M. Taipova |
| collection | DOAJ |
| description | Background. One of the important indicators of the nutritional value of amaranth is the high content of protein and lipids in seeds. Hence, obtaining and identifying such forms of amaranth through breeding, so that they also possessed resistance to abiotic stressors, is an important task.Materials and methods. Leaves and seeds of Amaranthus cruentus L. and mutants of the second inbred generation obtained by treatment with sodium azide were analyzed. The Bradford assay was used to measure the content of total soluble protein, lipid analysis was performed by thin-layer chromatography, the state of the antioxidant system was assessed according to catalase and peroxidase activities and the rate of superoxide anion formation. Mathematical data were processed using the Statistica 10.0 software.Results. The highest concentration of total protein in seeds was 13.78 mg/g in one of the mutants obtained after treatment with 3 mM sodium azide. Fifteen fatty acids were found in amaranth seeds, and in four mutants a significant increase in the percentage of omega-6 unsaturated linoleic acid was recorded. An increase in salt tolerance compared to the control was observed in mutants No. 2 and No. 3. Mutant No. 2 under salinization demonstrated higher peroxidase activity and mutant No. 3 higher catalase activity; both mutants showed a reduced rate of superoxide anion formation compared to the control.Conclusion. Amaranth mutants identified for higher stress resistance, protein content and linoleic acid content can be recommended for further breeding to produce new cultivars of amaranth with economically valuable traits. |
| format | Article |
| id | doaj-art-9c5b63ddd70e4d93bdfb289977f8f03c |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-9c5b63ddd70e4d93bdfb289977f8f03c2025-02-03T08:31:14ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822022-04-011831768510.30901/2227-8834-2022-1-76-85579Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L.R. M. Taipova0V. N. Nesterov1O. A. Rozentsvet2B. R. Kuluev3Bashkir State UniversityInstitute of Ecology of the Volga Basin of the Russian Academy of Sciences, branch of Samara Federal Research Center of the Russian Academy of SciencesInstitute of Ecology of the Volga Basin of the Russian Academy of Sciences, branch of Samara Federal Research Center of the Russian Academy of SciencesInstitute of Biochemistry and Genetics, subdivision of Ufa Federal Research Centre of the Russian Academy of SciencesBackground. One of the important indicators of the nutritional value of amaranth is the high content of protein and lipids in seeds. Hence, obtaining and identifying such forms of amaranth through breeding, so that they also possessed resistance to abiotic stressors, is an important task.Materials and methods. Leaves and seeds of Amaranthus cruentus L. and mutants of the second inbred generation obtained by treatment with sodium azide were analyzed. The Bradford assay was used to measure the content of total soluble protein, lipid analysis was performed by thin-layer chromatography, the state of the antioxidant system was assessed according to catalase and peroxidase activities and the rate of superoxide anion formation. Mathematical data were processed using the Statistica 10.0 software.Results. The highest concentration of total protein in seeds was 13.78 mg/g in one of the mutants obtained after treatment with 3 mM sodium azide. Fifteen fatty acids were found in amaranth seeds, and in four mutants a significant increase in the percentage of omega-6 unsaturated linoleic acid was recorded. An increase in salt tolerance compared to the control was observed in mutants No. 2 and No. 3. Mutant No. 2 under salinization demonstrated higher peroxidase activity and mutant No. 3 higher catalase activity; both mutants showed a reduced rate of superoxide anion formation compared to the control.Conclusion. Amaranth mutants identified for higher stress resistance, protein content and linoleic acid content can be recommended for further breeding to produce new cultivars of amaranth with economically valuable traits.https://elpub.vir.nw.ru/jour/article/view/1193chemical mutagenesissodium azidelipids and fatty acidslinoleic acidtotal proteinsalt stresscatalasesperoxidasessuperoxide anion |
| spellingShingle | R. M. Taipova V. N. Nesterov O. A. Rozentsvet B. R. Kuluev Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L. Труды по прикладной ботанике, генетике и селекции chemical mutagenesis sodium azide lipids and fatty acids linoleic acid total protein salt stress catalases peroxidases superoxide anion |
| title | Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L. |
| title_full | Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L. |
| title_fullStr | Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L. |
| title_full_unstemmed | Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L. |
| title_short | Changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of <i>Amaranthus cruentus</i> L. |
| title_sort | changes in the content of proteins and lipids and in the state of the antioxidant system in mutant forms of i amaranthus cruentus i l |
| topic | chemical mutagenesis sodium azide lipids and fatty acids linoleic acid total protein salt stress catalases peroxidases superoxide anion |
| url | https://elpub.vir.nw.ru/jour/article/view/1193 |
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