Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite
Bone graft substitutes and cancellous biomaterials have been widely used to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is widely used in reconstructive bone surgery owing to its biocompatibility. In addition, the...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2010/456240 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567601652301824 |
|---|---|
| author | Lorenzo Fassina Enrica Saino Maria Gabriella Cusella De Angelis Giovanni Magenes Francesco Benazzo Livia Visai |
| author_facet | Lorenzo Fassina Enrica Saino Maria Gabriella Cusella De Angelis Giovanni Magenes Francesco Benazzo Livia Visai |
| author_sort | Lorenzo Fassina |
| collection | DOAJ |
| description | Bone graft substitutes and cancellous biomaterials have been widely used to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is widely used in reconstructive bone surgery owing to its biocompatibility. In addition, the in vitro modification of cancellous hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study, we have followed a tissue-engineering strategy where ultrasonically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix inside a porous hydroxyapatite scaffold. The ultrasonic stimulus had the following parameters: average power equal to 149 mW and frequency of 1.5 MHz. In comparison with control conditions, the ultrasonic stimulus increased the cell proliferation and the surface coating with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The mechanical stimulus aimed at obtaining a better modification of the biomaterial internal surface in terms of cell colonization and coating with bone matrix. The modified biomaterial could be used, in clinical applications, as an implant for bone repair. |
| format | Article |
| id | doaj-art-9c5b2009774e436caf0ea73a8d1f060c |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-9c5b2009774e436caf0ea73a8d1f060c2025-02-03T01:00:59ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2010-01-01201010.1155/2010/456240456240Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous HydroxyapatiteLorenzo Fassina0Enrica Saino1Maria Gabriella Cusella De Angelis2Giovanni Magenes3Francesco Benazzo4Livia Visai5Dipartimento di Informatica e Sistemistica, University of Pavia, 27100 Pavia, ItalyCentre for Tissue Engineering (C.I.T.), University of Pavia, 27100 Pavia, ItalyCentre for Tissue Engineering (C.I.T.), University of Pavia, 27100 Pavia, ItalyDipartimento di Informatica e Sistemistica, University of Pavia, 27100 Pavia, ItalyCentre for Tissue Engineering (C.I.T.), University of Pavia, 27100 Pavia, ItalyCentre for Tissue Engineering (C.I.T.), University of Pavia, 27100 Pavia, ItalyBone graft substitutes and cancellous biomaterials have been widely used to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is widely used in reconstructive bone surgery owing to its biocompatibility. In addition, the in vitro modification of cancellous hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study, we have followed a tissue-engineering strategy where ultrasonically stimulated SAOS-2 human osteoblasts proliferated and built their extracellular matrix inside a porous hydroxyapatite scaffold. The ultrasonic stimulus had the following parameters: average power equal to 149 mW and frequency of 1.5 MHz. In comparison with control conditions, the ultrasonic stimulus increased the cell proliferation and the surface coating with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The mechanical stimulus aimed at obtaining a better modification of the biomaterial internal surface in terms of cell colonization and coating with bone matrix. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.http://dx.doi.org/10.1155/2010/456240 |
| spellingShingle | Lorenzo Fassina Enrica Saino Maria Gabriella Cusella De Angelis Giovanni Magenes Francesco Benazzo Livia Visai Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite Bioinorganic Chemistry and Applications |
| title | Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite |
| title_full | Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite |
| title_fullStr | Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite |
| title_full_unstemmed | Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite |
| title_short | Low-Power Ultrasounds as a Tool to Culture Human Osteoblasts inside Cancellous Hydroxyapatite |
| title_sort | low power ultrasounds as a tool to culture human osteoblasts inside cancellous hydroxyapatite |
| url | http://dx.doi.org/10.1155/2010/456240 |
| work_keys_str_mv | AT lorenzofassina lowpowerultrasoundsasatooltoculturehumanosteoblastsinsidecancelloushydroxyapatite AT enricasaino lowpowerultrasoundsasatooltoculturehumanosteoblastsinsidecancelloushydroxyapatite AT mariagabriellacuselladeangelis lowpowerultrasoundsasatooltoculturehumanosteoblastsinsidecancelloushydroxyapatite AT giovannimagenes lowpowerultrasoundsasatooltoculturehumanosteoblastsinsidecancelloushydroxyapatite AT francescobenazzo lowpowerultrasoundsasatooltoculturehumanosteoblastsinsidecancelloushydroxyapatite AT liviavisai lowpowerultrasoundsasatooltoculturehumanosteoblastsinsidecancelloushydroxyapatite |