Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells
Dysregulated lipid metabolism in liver is an important hallmark of non-alcoholic fatty liver disease (NAFLD), which may be modulated by dietary polyphenols or microRNAs (miRNAs). However, the underlying epigenetic regulatory mechanism of polyphenols remain unclear. The current study aimed to address...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2024-02-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Curcumin-Prevents-Free-Fatty-Acid-Induced-Lipid-Accumulation-via-Targeting-the-miR,182927,0,2.html |
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| author | Yuanyuan Mei Xiaoting Sun Shi-Ying Huang Xiaowen Wu Kuo-Ting Ho Liming Lu Chaoxiang Chen Jian Li Jingwen Liu Guiling Li |
| author_facet | Yuanyuan Mei Xiaoting Sun Shi-Ying Huang Xiaowen Wu Kuo-Ting Ho Liming Lu Chaoxiang Chen Jian Li Jingwen Liu Guiling Li |
| author_sort | Yuanyuan Mei |
| collection | DOAJ |
| description | Dysregulated lipid metabolism in liver is an important hallmark of non-alcoholic fatty liver disease (NAFLD), which may be modulated by dietary polyphenols or microRNAs (miRNAs). However, the underlying epigenetic regulatory mechanism of polyphenols remain unclear. The current study aimed to address how miRNA mediates hepatic lipid metabolic control of curcumin, a polyphenolic food supplement. The results showed that 24 h treatment with 5 - 20 μM curcumin prevented free fatty acid-induced lipid accumulation by around 10 ~ 50% in HepG2 cells, which was attenuated by pre-transfection with 40 nM miR-22-3p mimic for 48 h. In consequence, transfection with 40 nM miR-22-3p inhibitor for 48 h significantly reduced lipid accumulation by around 10%. And 48 h overexpression of miR-22-3p targeting cardiolipin synthase 1 ( CRLS1 ) gene, which encodes a mitochondrial phospholipid synthase, showed a similar regulatory effect. Thus, miR-22-3p and CRLS1 showed opposite effects in modulating lipid metabolism, which probably involved mitochondrial control. In summary, this study demonstrated that curcumin improved hepatic lipid metabolism via targeting the miR-22-3p/ CRLS1 pathway. Identification of the epigenetic regulatory mechanism underlying lipid metabolism may thereby facilitate alleviation of metabolic disorders by natural polyphenols. |
| format | Article |
| id | doaj-art-9c53c3455a5b4f2f86bfcaafddab8ef9 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-9c53c3455a5b4f2f86bfcaafddab8ef92025-02-02T23:57:21ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072024-02-01741596810.31883/pjfns/182927182927Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 CellsYuanyuan Mei0https://orcid.org/0009-0007-7216-3983Xiaoting Sun1https://orcid.org/0000-0001-7616-8991Shi-Ying Huang2https://orcid.org/0000-0002-0370-1553Xiaowen Wu3Kuo-Ting Ho4https://orcid.org/0000-0001-7224-1338Liming Lu5https://orcid.org/0000-0002-2544-966XChaoxiang Chen6https://orcid.org/0000-0003-2147-5219Jian Li7https://orcid.org/0000-0002-3444-3788Jingwen Liu8https://orcid.org/0000-0001-7231-6483Guiling Li9https://orcid.org/0000-0002-0940-6010College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaHI. Q Biomedical Laboratory, Taiwan Investment Zone, Quanzhou, Fujian, 362123, P. R. ChinaShanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, P. R. ChinaDysregulated lipid metabolism in liver is an important hallmark of non-alcoholic fatty liver disease (NAFLD), which may be modulated by dietary polyphenols or microRNAs (miRNAs). However, the underlying epigenetic regulatory mechanism of polyphenols remain unclear. The current study aimed to address how miRNA mediates hepatic lipid metabolic control of curcumin, a polyphenolic food supplement. The results showed that 24 h treatment with 5 - 20 μM curcumin prevented free fatty acid-induced lipid accumulation by around 10 ~ 50% in HepG2 cells, which was attenuated by pre-transfection with 40 nM miR-22-3p mimic for 48 h. In consequence, transfection with 40 nM miR-22-3p inhibitor for 48 h significantly reduced lipid accumulation by around 10%. And 48 h overexpression of miR-22-3p targeting cardiolipin synthase 1 ( CRLS1 ) gene, which encodes a mitochondrial phospholipid synthase, showed a similar regulatory effect. Thus, miR-22-3p and CRLS1 showed opposite effects in modulating lipid metabolism, which probably involved mitochondrial control. In summary, this study demonstrated that curcumin improved hepatic lipid metabolism via targeting the miR-22-3p/ CRLS1 pathway. Identification of the epigenetic regulatory mechanism underlying lipid metabolism may thereby facilitate alleviation of metabolic disorders by natural polyphenols.http://journal.pan.olsztyn.pl/Curcumin-Prevents-Free-Fatty-Acid-Induced-Lipid-Accumulation-via-Targeting-the-miR,182927,0,2.htmlpolyphenolepigeneticsliversteatosismitochondria |
| spellingShingle | Yuanyuan Mei Xiaoting Sun Shi-Ying Huang Xiaowen Wu Kuo-Ting Ho Liming Lu Chaoxiang Chen Jian Li Jingwen Liu Guiling Li Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells Polish Journal of Food and Nutrition Sciences polyphenol epigenetics liver steatosis mitochondria |
| title | Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells |
| title_full | Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells |
| title_fullStr | Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells |
| title_full_unstemmed | Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells |
| title_short | Curcumin Prevents Free Fatty Acid-Induced Lipid Accumulation via Targeting the miR-22-3p/ CRLS1 Pathway in HepG2 Cells |
| title_sort | curcumin prevents free fatty acid induced lipid accumulation via targeting the mir 22 3p crls1 pathway in hepg2 cells |
| topic | polyphenol epigenetics liver steatosis mitochondria |
| url | http://journal.pan.olsztyn.pl/Curcumin-Prevents-Free-Fatty-Acid-Induced-Lipid-Accumulation-via-Targeting-the-miR,182927,0,2.html |
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