Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves

Limiting and/or slowing down the starch digestion process and consequently the release of glucose can be an important strategy for the prevention of type 2 diabetes (T2D). The aim of the current in vitro study was to assess the anti-diabetic and antioxidant potential of red radish leaves of the Carm...

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Main Authors: Dominika Kajszczak, Dorota Sosnowska, Barbara Frąszczak, Anna Podsędek
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/23/5689
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author Dominika Kajszczak
Dorota Sosnowska
Barbara Frąszczak
Anna Podsędek
author_facet Dominika Kajszczak
Dorota Sosnowska
Barbara Frąszczak
Anna Podsędek
author_sort Dominika Kajszczak
collection DOAJ
description Limiting and/or slowing down the starch digestion process and consequently the release of glucose can be an important strategy for the prevention of type 2 diabetes (T2D). The aim of the current in vitro study was to assess the anti-diabetic and antioxidant potential of red radish leaves of the Carmen, Jutrzenka, Saxa, and Warta cultivars. In the context of anti-diabetic activity, the effect of leaves on potato starch digestion and free glucose binding, as well as inhibitory effects of leaf extracts against α-amylase and α-glucosidase and non-enzymatic glycation (AGEs) were determined. The basic chemical composition, quantitative composition of phenolic compounds, and antioxidant activity of leaves were also estimated. This study showed that all radish leaves inhibited the breakdown of potato starch and showed their ability to bind glucose. This activity was correlated with the content of hydroxycinnamic acids, protein and dietary fiber while flavones was probably responsible for glucose binding. Leaf extracts inhibited α-glucosidase activity and formation of AGEs but were practically inactive towards α-amylase. Inhibition of α-glucosidase activity was related to the content of proanthocyanidins and inhibition of AGEs formation to flavonols. These results point to radish leaves, especially the Warta and Jutrzenka cultivars, as a potential natural remedy for treating T2D.
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spelling doaj-art-9c0653006bfc4e748fdfd7ed72bd8bde2025-08-20T02:50:34ZengMDPI AGMolecules1420-30492024-12-012923568910.3390/molecules29235689Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> LeavesDominika Kajszczak0Dorota Sosnowska1Barbara Frąszczak2Anna Podsędek3Institute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Łódź, PolandInstitute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Łódź, PolandDepartment of Vegetable Crops, Poznań University of Life Sciences, Dąbrowskiego 159, 60-594 Poznań, PolandInstitute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Łódź, PolandLimiting and/or slowing down the starch digestion process and consequently the release of glucose can be an important strategy for the prevention of type 2 diabetes (T2D). The aim of the current in vitro study was to assess the anti-diabetic and antioxidant potential of red radish leaves of the Carmen, Jutrzenka, Saxa, and Warta cultivars. In the context of anti-diabetic activity, the effect of leaves on potato starch digestion and free glucose binding, as well as inhibitory effects of leaf extracts against α-amylase and α-glucosidase and non-enzymatic glycation (AGEs) were determined. The basic chemical composition, quantitative composition of phenolic compounds, and antioxidant activity of leaves were also estimated. This study showed that all radish leaves inhibited the breakdown of potato starch and showed their ability to bind glucose. This activity was correlated with the content of hydroxycinnamic acids, protein and dietary fiber while flavones was probably responsible for glucose binding. Leaf extracts inhibited α-glucosidase activity and formation of AGEs but were practically inactive towards α-amylase. Inhibition of α-glucosidase activity was related to the content of proanthocyanidins and inhibition of AGEs formation to flavonols. These results point to radish leaves, especially the Warta and Jutrzenka cultivars, as a potential natural remedy for treating T2D.https://www.mdpi.com/1420-3049/29/23/5689leavesred radishstarch digestionα-amylaseα-glucosidaseAGEs
spellingShingle Dominika Kajszczak
Dorota Sosnowska
Barbara Frąszczak
Anna Podsędek
Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves
Molecules
leaves
red radish
starch digestion
α-amylase
α-glucosidase
AGEs
title Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves
title_full Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves
title_fullStr Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves
title_full_unstemmed Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves
title_short Composition, Anti-Diabetic, and Antioxidant Potential of <i>Raphanus sativus</i> Leaves
title_sort composition anti diabetic and antioxidant potential of i raphanus sativus i leaves
topic leaves
red radish
starch digestion
α-amylase
α-glucosidase
AGEs
url https://www.mdpi.com/1420-3049/29/23/5689
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