Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells
Cardiospheres (CSs) are self-assembling multicellular clusters from the cellular outgrowth from cardiac explants cultured in nonadhesive substrates. They contain a core of primitive, proliferating cells, and an outer layer of mesenchymal/stromal cells and differentiating cells that express cardiomyo...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2013/916837 |
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| author | Lucio Barile Mihaela Gherghiceanu Laurenţiu M. Popescu Tiziano Moccetti Giuseppe Vassalli |
| author_facet | Lucio Barile Mihaela Gherghiceanu Laurenţiu M. Popescu Tiziano Moccetti Giuseppe Vassalli |
| author_sort | Lucio Barile |
| collection | DOAJ |
| description | Cardiospheres (CSs) are self-assembling multicellular clusters from the cellular outgrowth from cardiac explants cultured in nonadhesive substrates. They contain a core of primitive, proliferating cells, and an outer layer of mesenchymal/stromal cells and differentiating cells that express cardiomyocyte proteins and connexin 43. Because CSs contain both primitive cells and committed progenitors for the three major cell types present in the heart, that is, cardiomyocytes, endothelial cells, and smooth muscle cells, and because they are derived from percutaneous endomyocardial biopsies, they represent an attractive cell source for cardiac regeneration. In preclinical studies, CS-derived cells (CDCs) delivered to infarcted hearts resulted in improved cardiac function. CDCs have been tested safely in an initial phase-1 clinical trial in patients after myocardial infarction. Whether or not CDCs are superior to purified populations, for example, c-kit+ cardiac stem cells, or to gene therapy approaches for cardiac regeneration remains to be evaluated. |
| format | Article |
| id | doaj-art-9c04354e32504a3d878c6d292470cd50 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9c04354e32504a3d878c6d292470cd502025-02-03T01:27:36ZengWileyStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/916837916837Human Cardiospheres as a Source of Multipotent Stem and Progenitor CellsLucio Barile0Mihaela Gherghiceanu1Laurenţiu M. Popescu2Tiziano Moccetti3Giuseppe Vassalli4Molecular Cardiology Laboratory and Department of Cardiology, Fondazione Cardiocentro Ticino, Via Tesserete 48, 6900 Lugano, SwitzerlandUltrastructural Pathology, “Victor Babeş” National Institute of Pathology, 99-101 Splainl Independentei, 050096 Bucharest 5, RomaniaUltrastructural Pathology, “Victor Babeş” National Institute of Pathology, 99-101 Splainl Independentei, 050096 Bucharest 5, RomaniaMolecular Cardiology Laboratory and Department of Cardiology, Fondazione Cardiocentro Ticino, Via Tesserete 48, 6900 Lugano, SwitzerlandMolecular Cardiology Laboratory and Department of Cardiology, Fondazione Cardiocentro Ticino, Via Tesserete 48, 6900 Lugano, SwitzerlandCardiospheres (CSs) are self-assembling multicellular clusters from the cellular outgrowth from cardiac explants cultured in nonadhesive substrates. They contain a core of primitive, proliferating cells, and an outer layer of mesenchymal/stromal cells and differentiating cells that express cardiomyocyte proteins and connexin 43. Because CSs contain both primitive cells and committed progenitors for the three major cell types present in the heart, that is, cardiomyocytes, endothelial cells, and smooth muscle cells, and because they are derived from percutaneous endomyocardial biopsies, they represent an attractive cell source for cardiac regeneration. In preclinical studies, CS-derived cells (CDCs) delivered to infarcted hearts resulted in improved cardiac function. CDCs have been tested safely in an initial phase-1 clinical trial in patients after myocardial infarction. Whether or not CDCs are superior to purified populations, for example, c-kit+ cardiac stem cells, or to gene therapy approaches for cardiac regeneration remains to be evaluated.http://dx.doi.org/10.1155/2013/916837 |
| spellingShingle | Lucio Barile Mihaela Gherghiceanu Laurenţiu M. Popescu Tiziano Moccetti Giuseppe Vassalli Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells Stem Cells International |
| title | Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells |
| title_full | Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells |
| title_fullStr | Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells |
| title_full_unstemmed | Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells |
| title_short | Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells |
| title_sort | human cardiospheres as a source of multipotent stem and progenitor cells |
| url | http://dx.doi.org/10.1155/2013/916837 |
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