Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test
While deficient brain plasticity is a well-established pathophysiologic feature of depression, little is known about disorder-associated enhanced cognitive processing. Here, we studied a novel mouse paradigm that potentially models augmented learning of adverse memories during development of a depre...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/5098591 |
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| author | Tatyana Strekalova Nataliia Markova Elena Shevtsova Olga Zubareva Anastassia Bakhmet Harry M. Steinbusch Sergey Bachurin Klaus-Peter Lesch |
| author_facet | Tatyana Strekalova Nataliia Markova Elena Shevtsova Olga Zubareva Anastassia Bakhmet Harry M. Steinbusch Sergey Bachurin Klaus-Peter Lesch |
| author_sort | Tatyana Strekalova |
| collection | DOAJ |
| description | While deficient brain plasticity is a well-established pathophysiologic feature of depression, little is known about disorder-associated enhanced cognitive processing. Here, we studied a novel mouse paradigm that potentially models augmented learning of adverse memories during development of a depressive-like state. We used a modification of the classic two-day protocol of a mouse Porsolt test with an additional session occurring on Day 5 following the initial exposure. Unexpectedly, floating behaviour and brain glycogen synthase kinase-3 beta (GSK-3beta) mRNA levels, a factor of synaptic plasticity as well as a marker of distress and depression, were increased during the additional swimming session that was prevented by imipramine. Observed increases of GSK-3beta mRNA in prefrontal cortex during delayed testing session correlated with individual parameters of behavioural despair that was not found in the classic Porsolt test. Repeated swim exposure was accompanied by a lower pGSK-3beta/GSK-3beta ratio. A replacement of the second or the final swim sessions with exposure to the context of testing resulted in increased GSK-3beta mRNA level similar to the effects of swimming, while exclusion of the second testing prevented these changes. Together, our findings implicate the activation of brain GSK-3beta expression in enhanced contextual conditioning of adverse memories, which is associated with an individual susceptibility to a depressive syndrome. |
| format | Article |
| id | doaj-art-9bfc2ef107d94e8fbbef536270f1f92a |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-9bfc2ef107d94e8fbbef536270f1f92a2025-02-03T06:00:46ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/50985915098591Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim TestTatyana Strekalova0Nataliia Markova1Elena Shevtsova2Olga Zubareva3Anastassia Bakhmet4Harry M. Steinbusch5Sergey Bachurin6Klaus-Peter Lesch7Department of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, NetherlandsDepartment of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, NetherlandsLaboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severnii Proezd 1, Chernogolovka, Moscow 142432, RussiaDepartment of Physiology, Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”, Akademika Pavlova Street 12, Saint-Petersburg 197022, RussiaDepartment of Human Anatomy, I.M. Sechenov First Moscow State Medical University, Mokhovaya Street 11-10, Moscow 125009, RussiaDepartment of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, NetherlandsLaboratory of Biomolecular Screening, Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severnii Proezd 1, Chernogolovka, Moscow 142432, RussiaDepartment of Neuroscience, School for Mental Health and Neuroscience, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, NetherlandsWhile deficient brain plasticity is a well-established pathophysiologic feature of depression, little is known about disorder-associated enhanced cognitive processing. Here, we studied a novel mouse paradigm that potentially models augmented learning of adverse memories during development of a depressive-like state. We used a modification of the classic two-day protocol of a mouse Porsolt test with an additional session occurring on Day 5 following the initial exposure. Unexpectedly, floating behaviour and brain glycogen synthase kinase-3 beta (GSK-3beta) mRNA levels, a factor of synaptic plasticity as well as a marker of distress and depression, were increased during the additional swimming session that was prevented by imipramine. Observed increases of GSK-3beta mRNA in prefrontal cortex during delayed testing session correlated with individual parameters of behavioural despair that was not found in the classic Porsolt test. Repeated swim exposure was accompanied by a lower pGSK-3beta/GSK-3beta ratio. A replacement of the second or the final swim sessions with exposure to the context of testing resulted in increased GSK-3beta mRNA level similar to the effects of swimming, while exclusion of the second testing prevented these changes. Together, our findings implicate the activation of brain GSK-3beta expression in enhanced contextual conditioning of adverse memories, which is associated with an individual susceptibility to a depressive syndrome.http://dx.doi.org/10.1155/2016/5098591 |
| spellingShingle | Tatyana Strekalova Nataliia Markova Elena Shevtsova Olga Zubareva Anastassia Bakhmet Harry M. Steinbusch Sergey Bachurin Klaus-Peter Lesch Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test Neural Plasticity |
| title | Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test |
| title_full | Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test |
| title_fullStr | Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test |
| title_full_unstemmed | Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test |
| title_short | Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test |
| title_sort | individual differences in behavioural despair predict brain gsk 3beta expression in mice the power of a modified swim test |
| url | http://dx.doi.org/10.1155/2016/5098591 |
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