Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder
This study investigates the neuroprotective effects of dexpanthenol (DEX) and butafosfan-vitmain B12 combination (BUT) in a circadian rhythm disorder (CRD)- induced brain damage model in mice. Control, CRD, DEX, BUT, and BUT + DEX groups were subjected to a 19-day experimental period during which CR...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2025-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3207 |
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| Summary: | This study investigates the neuroprotective effects of dexpanthenol (DEX) and
butafosfan-vitmain B12 combination (BUT) in a circadian rhythm disorder (CRD)-
induced brain damage model in mice. Control, CRD, DEX, BUT, and BUT + DEX
groups were subjected to a 19-day experimental period during which CRD was induced
by repeated phase shifts in the light/dark cycle. The CRD group experienced circadian
rhythm disorder, while the DEX and BUT groups received intraperitoneal DEX (1000
mg/kg/day) and subcutaneous BUT (200 mg/kg/day) treatments, respectively, for the
same duration. Serum cortisol and creatine kinase (CK) levels were measured using
ELISA to assess stress and tissue damage. Brain tissues were evaluated histopathologically
using hematoxylin and eosin staining, and immunohistochemically for brain-derived
neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) expression using
specific monoclonal antibodies. CRD significantly increased serum cortisol and CK
levels compared to the control group (P<0.001). Both DEX and BUT treatments reduced
these elevations, with the combination therapy showing the most pronounced effect
(P<0.001). Histopathological examination revealed reduced neuronal degeneration,
hyperemia, and hemorrhage in the treatment groups compared to the CRD group.
Immunohistochemical analysis showed significantly increased BDNF and GFAP
expression in the BUT + DEX group (P<0.001). These findings suggest that DEX and
BUT, particularly in combination, exert neuroprotective effects against CRD-induced
brain injury by modulating oxidative stress, inflammation, and neurotrophic signaling
pathways. |
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| ISSN: | 1309-2251 |