circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis
Background. The mortality rate of colorectal cancer (CRC) ranks second. circRNAs are abnormal expression in some diseases, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer is inseparable from the abnormal exp...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/8583382 |
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| author | Yandong Huang Qinyang Bai Zhanlong Wang Hongbo Yu Yanru Li Hao Lu Huimin Kang Xuewei Shi Kai Feng |
| author_facet | Yandong Huang Qinyang Bai Zhanlong Wang Hongbo Yu Yanru Li Hao Lu Huimin Kang Xuewei Shi Kai Feng |
| author_sort | Yandong Huang |
| collection | DOAJ |
| description | Background. The mortality rate of colorectal cancer (CRC) ranks second. circRNAs are abnormal expression in some diseases, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer is inseparable from the abnormal expression of circRNAs. Methods. First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by qRT-PCR. Then, we constructed circ_0052184-silenced CRC cells and detected by qRT-PCR. Furthermore, the proliferation ability of cells was detected by colony formation assay. Cell migration ability was tested by wound healing assay and transwell assay. Cell invasion ability was detected by transwell assay. Results. Expression of circ_0052184 was significantly increased in colorectal cancer cell lines and tissues. Silencing circ_0052184 affected the proliferation, migration, and invasion of colorectal cancer cells. miR-604 was targeted by circ_0052184. The downstream target of miR-604 was HOXA9, and silencing circ_0052184 inhibited HOXA9 expression. The existence of the circ_0052184/miR-604/HOXA9 regulatory network in colorectal cancer was validated. circ_0052184 promoted the occurrence and development of colorectal cancer by targeting the miR-604/HOXA9 axis. Conclusions. Our study revealed that the molecular mechanism of circ_0052184 regulated the miR-604/HOXA9 axis, which might promote the malignant progression of colorectal cancer cells. |
| format | Article |
| id | doaj-art-9be534c1595c4969ae4d4cd6d1c19780 |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-9be534c1595c4969ae4d4cd6d1c197802025-02-03T05:50:40ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/8583382circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 AxisYandong Huang0Qinyang Bai1Zhanlong Wang2Hongbo Yu3Yanru Li4Hao Lu5Huimin Kang6Xuewei Shi7Kai Feng8Department of Tumor CenterDepartment of Tumor CenterDepartment of Tumor CenterPractical Education Skills Center of Baotou Medical CollegeDepartment of Tumor CenterDepartment of Tumor CenterDepartment of Tumor CenterDepartment of Tumor CenterDepartment of Tumor CenterBackground. The mortality rate of colorectal cancer (CRC) ranks second. circRNAs are abnormal expression in some diseases, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer is inseparable from the abnormal expression of circRNAs. Methods. First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by qRT-PCR. Then, we constructed circ_0052184-silenced CRC cells and detected by qRT-PCR. Furthermore, the proliferation ability of cells was detected by colony formation assay. Cell migration ability was tested by wound healing assay and transwell assay. Cell invasion ability was detected by transwell assay. Results. Expression of circ_0052184 was significantly increased in colorectal cancer cell lines and tissues. Silencing circ_0052184 affected the proliferation, migration, and invasion of colorectal cancer cells. miR-604 was targeted by circ_0052184. The downstream target of miR-604 was HOXA9, and silencing circ_0052184 inhibited HOXA9 expression. The existence of the circ_0052184/miR-604/HOXA9 regulatory network in colorectal cancer was validated. circ_0052184 promoted the occurrence and development of colorectal cancer by targeting the miR-604/HOXA9 axis. Conclusions. Our study revealed that the molecular mechanism of circ_0052184 regulated the miR-604/HOXA9 axis, which might promote the malignant progression of colorectal cancer cells.http://dx.doi.org/10.1155/2022/8583382 |
| spellingShingle | Yandong Huang Qinyang Bai Zhanlong Wang Hongbo Yu Yanru Li Hao Lu Huimin Kang Xuewei Shi Kai Feng circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis Analytical Cellular Pathology |
| title | circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis |
| title_full | circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis |
| title_fullStr | circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis |
| title_full_unstemmed | circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis |
| title_short | circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis |
| title_sort | circ 0052184 promotes colorectal cancer progression via targeting mir 604 hoxa9 axis |
| url | http://dx.doi.org/10.1155/2022/8583382 |
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