Degradation of Natural <i>Undaria pinnatifida</i> into Unsaturated Guluronic Acid Oligosaccharides by a Single Alginate Lyase

Degradation of Natural <i>Undaria pinnatifida</i> into Unsaturated Guluronic Acid Oligosaccharides by a Single Alginate Lyase

Here, we report on a bifunctional alginate lyase (Vnalg7) expressed in <i>Pichia pastoris</i>, which can degrade natural <i>Undaria pinnatifida</i> into unsaturated guluronic acid di- and trisaccharide without pretreatment. The enzyme activity of Vnalg7 (3620.00 U/mL-culture)...

Full description

Saved in:

Bibliographic Details
Main Authors:	Hui Wang, Jiaqi Wen, Nuraliya Ablimit, Kun Deng, Wenzhuo Wang, Wei Jiang
Format:	Article
Language:	English
Published:	MDPI AG 2024-10-01
Series:	Marine Drugs
Subjects:	alginate lyase action mode alginate oligosaccharide catalytic mechanism <i>Undaria pinnatifida</i>
Online Access:	https://www.mdpi.com/1660-3397/22/10/453
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Expression, Purification, and Characterisation of Recombinant Alginate Lyase (<i>Flammeovirga</i> AL2) for the Bioconversion of Alginate into Alginate Oligosaccharides
by: Coleen E. Grobler, et al.
Published: (2024-11-01)

Direct Preparation of Alginate Oligosaccharides from Brown Algae by an Algae-Decomposing Alginate Lyase AlyP18 from the Marine Bacterium <i>Pseudoalteromonas agarivorans</i> A3
by: Xiao-Hui Sun, et al.
Published: (2024-10-01)

Therapeutic Prospects of <i>Undaria pinnatifida</i> Polysaccharides: Extraction, Purification, and Functional Activity
by: Kit-Leong Cheong, et al.
Published: (2025-04-01)

Multi-Functional Alginate Lyase <i>AlgVR7</i> from <i>Vibrio rumoiensis</i>: Structural Insights and Catalytic Mechanisms
by: Zhe Huang, et al.
Published: (2025-03-01)

The Identification and Characterization of a Novel Alginate Lyase from <i>Mesonia hitae</i> R32 Exhibiting High Thermal Stability and Potent Antioxidant Oligosaccharide Production
by: Yongshang Ye, et al.
Published: (2025-04-01)

Application of marine bacterial alginate lyases in wastewater treatment, biofilm removal and green technology
by: M.U.K. Anjung, et al.
Published: (2025-07-01)

Improvement of <i>Undaria pinnatifida</i> Sugar-Free Gummy Jellies’ Properties by Phycocyanin Under Ultraviolet (UV) Irradiation
by: Ying Bai, et al.
Published: (2024-12-01)

Combined Effects of Marine Heatwaves and Light Intensity on the Physiological, Transcriptomic, and Metabolomic Profiles of <i>Undaria pinnatifida</i>
by: Hanmo Song, et al.
Published: (2025-05-01)

Neuroprotective and Anti-Inflammatory Activity of <i>Undaria pinnatifida</i> Fucoidan In Vivo—A Proteomic Investigation
by: Cheng Yang, et al.
Published: (2025-04-01)

Improvement of Catalytic Activity and Thermostability of Alginate Lyase VxAly7B-CM via Rational Computational Design Strategies
by: Xin Ma, et al.
Published: (2025-05-01)

Recent Advances in Alginate Lyase Engineering for Efficient Conversion of Alginate to Value‐Added Products
by: Hyo Jeong Shin, et al.
Published: (2025-05-01)

Fucoidan from <i>Undaria pinnatifida</i> Enhances Exercise Performance and Increases the Abundance of Beneficial Gut Bacteria in Mice
by: Cheng Yang, et al.
Published: (2024-10-01)

Current Distribution of the Invasive Kelp <i>Undaria pinnatifida</i> (Harvey) Suringar, 1873 Along Artificial and Natural Habitats in North Portugal—Impacts and Mitigation Initiatives
by: Marcos Rubal, et al.
Published: (2025-02-01)

Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate
by: Xiao Liu, et al.
Published: (2024-12-01)

<i>Undaria pinnatifida</i> Fucoidan Enhances Gut Microbiome, Butyrate Production, and Exerts Anti-Inflammatory Effects in an In Vitro Short-Term SHIME<sup>®</sup> Coupled to a Caco-2/THP-1 Co-Culture Model
by: Barbara C. Wimmer, et al.
Published: (2025-06-01)

Antioxidant and immunomodulatory activity of Perna canaliculus oil extract in combination with low molecular weight fucoidan extracted from Undaria pinnatifida
by: Belgheis Ebrahimi, et al.
Published: (2025-06-01)

Alginate-Degrading Modes, Oligosaccharide-Yielding Properties, and Potential Applications of a Novel Bacterial Multifunctional Enzyme, Aly16-1
by: Lianghuan Zeng, et al.
Published: (2024-11-01)

Perception and acceptance of high seaweed content novel foods (Ulva spp. and Undaria pinnatifida) across New Zealand and Singaporean consumers
by: Ao Chen, et al.
Published: (2024-12-01)

Exploring the Catalytic Mechanisms of a Newly Identified Salt-Activated Alginate Lyase from <i>Pseudoalteromonas carrageenovora</i> ASY5
by: Xiaoyan Zhuang, et al.
Published: (2025-06-01)

Characterization of alginate-degrading bacteria isolated from seaweed-producing areas of South Korean territory and verification of the bacteria as plant growth-promoting biofertilizer
by: Seung Hwa Jeong, et al.
Published: (2025-06-01)

Undaria pinnatifida extract attenuates combined allergic rhinitis and asthma syndrome by the modulation of epithelial cell dysfunction and oxidative stress
by: Yu Zhen Nan, et al.
Published: (2024-12-01)

Production, characterization and antioxidant analysis on the Undaria-based alcoholic beverages using response surface method and HS-SPME-GC × GC-TOF-MS
by: Fangru Nan, et al.
Published: (2025-04-01)

Alginate lyase immobilized Chlamydomonas algae microrobots: minimally invasive therapy for biofilm penetration and eradication
by: Xiaoting Zhang, et al.
Published: (2025-06-01)

Screening and identification of marine alginate lyase-producing bacteria and optimization of enzyme production conditions
by: HU Mengdi, LI Yaozu, ZHANG Xiaoyong, MO Meiqing, GAO Xiangyang
Published: (2025-04-01)

A comparative study of the efficacy of alginate lyases in the presence of metal ions elevated in the cystic fibrosis lung milieu
by: Neetu, et al.
Published: (2024-12-01)

Screening, Optimization of Fermentation Conditions and Evaluation of Sargassum-degrading Effect of Exiguobacterium mexicanum MAL070 Producing Alginate Lyase
by: Shuang LUO, et al.
Published: (2025-08-01)

Analysis of Flavor Differences Between <i>Undaria pinnatifida</i> Produced Using Different Processing Methods and from Different Origins Based on GC-IMS
by: Xinyi Che, et al.
Published: (2025-06-01)

Ameliorative Effect of Alginate Oligosaccharides on NG-nitro-L-arginine methyl ester-Induced Hypertension in Mice
by: CHEN Junbo, LI Yanxiao, YAN Qiaojuan, JIANG Jun, YANG Shaoqing, JIANG Zhengqiang,
Published: (2024-11-01)

Identification, Whole-Genome Sequencing and Analysis of Vibrio alginolyticus S10, a Strain Producing Alginate Lyase
by: LIU Fang, SHU Zhiqiang, WANG Gongming, JING Yuexin, ZHAO Yunping, XU Yingjiang, JIAO Chunna, ZHANG Jian
Published: (2024-11-01)

Insights into putative alginate lyases from epipelagic and mesopelagic communities of the global ocean
by: Mariana Lozada, et al.
Published: (2025-03-01)

Metabolic potential of Azotobacter alginate producers and sustainable alternatives for alginate extraction
by: Simiat O. Jimoh, et al.
Published: (2024-06-01)

Isolation and identification of an alginate lyase-producing marine bacterium strain and its enzymological characteristics
by: TANG Haiqing, et al.
Published: (2013-07-01)

Alginate Oligosaccharide Promoted the Nutrient Uptake and Growth of Cucumber Seedlings Under Suboptimal Temperature Conditions
by: Xu Guo, et al.
Published: (2025-05-01)

Effects of Combined Application of Alginate Oligosaccharides, Potassium Fertilizer and Water Management on the Yield and Quality of Citrus Fruits
by: Sijie SONG, et al.
Published: (2025-03-01)

Sodium Alginate/Cuprous Oxide Composite Materials with Antibacterial Properties: A Preliminary Study Revealing the Counteracting Effects of Oligosaccharides in the Matrix
by: Reeba Thomas, et al.
Published: (2025-05-01)

Effects of alginate oligosaccharides on depression-like behavior and gut microbiota in zebrafish induced by chronic unpredictable mild stress
by: LONG Yan, et al.
Published: (2024-07-01)

Removal of As from Tambo River Using Sodium Alginate from <i>Lessonia trabeculata</i> (Aracanto)
by: Diana M. Villanueva, et al.
Published: (2025-07-01)

Synergistic effects of alginate oligosaccharide and cyanidin-3-O-glucoside on the amelioration of intestinal barrier function in mice
by: Jie Li, et al.
Published: (2023-11-01)

Identification and Characterization of a Highly Active Hyaluronan Lyase from <em>Enterobacter asburiae</em>
by: Linjing Zhang, et al.
Published: (2024-08-01)

Effects of alginate oligosaccharides on the growth performance, hepatocyte health, and intestinal inflammation of the Chinese sea bass (Lateolabrax maculatus)
by: Shengzhe An, et al.
Published: (2025-03-01)