Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth
Plant growth-promoting rhizobacteria (PGPR) are an important bacterial resource as biofertilizers, which can promote plant growth and increase crop yields. In this study, isolation, characterization, and its effect on plant promotion were assessed by isolating PGPR using soil samples collected from...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2022/5115875 |
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| author | Yasin Ahmed Waday Ermias Girma Aklilu Mohammed Seid Bultum Venkata Ramayya Ancha Dejene Beyene |
| author_facet | Yasin Ahmed Waday Ermias Girma Aklilu Mohammed Seid Bultum Venkata Ramayya Ancha Dejene Beyene |
| author_sort | Yasin Ahmed Waday |
| collection | DOAJ |
| description | Plant growth-promoting rhizobacteria (PGPR) are an important bacterial resource as biofertilizers, which can promote plant growth and increase crop yields. In this study, isolation, characterization, and its effect on plant promotion were assessed by isolating PGPR using soil samples collected from Jimma, Ethiopia. Out of 10 isolates, three of them (JEC3, JEC4, and JEC7) exhibited PSB traits in Pikovskaya media by solubilizing phosphate and producing IAA. Of the three isolates, JEC4 recorded the maximum phosphate solubilization index (4.98), soluble phosphate (283 μg/ml), and IAA production (10.21 μg/ml). The findings of the isolates' morphological and biochemical tests showed that JEC3, JEC4, and JEC7 as Bacillus sp., Pseudomonas sp., and Enterobacter sp., respectively. The optimization of phosphate solubilization was done using different incubation times and sources of carbon and nitrogen. Furthermore, the isolate (JEC4) was tested for plant growth-promoting (PGP) activities and indicated the production of ammonia, hydrogen cyanide, and siderophore. JEC4 isolate showed a significant increase in sideways (%) seed germination, root and shoot length, fresh and dry weight of root, and shoot of maize in field experiments than the untreated ones. The results indicate that the JEC4 isolate can be developed as a biological fertilizer for promoting crop productivity and ecosystem dynamics. |
| format | Article |
| id | doaj-art-9bb746fdd8fc48e4a59f7c6efbf2394a |
| institution | Kabale University |
| issn | 1687-7675 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
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| series | Applied and Environmental Soil Science |
| spelling | doaj-art-9bb746fdd8fc48e4a59f7c6efbf2394a2025-02-03T01:32:31ZengWileyApplied and Environmental Soil Science1687-76752022-01-01202210.1155/2022/5115875Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize GrowthYasin Ahmed Waday0Ermias Girma Aklilu1Mohammed Seid Bultum2Venkata Ramayya Ancha3Dejene Beyene4School of Chemical EngineeringSchool of Chemical EngineeringSchool of Chemical EngineeringFaculty of Mechanical EngineeringFaculty of Civil and Environmental EngineeringPlant growth-promoting rhizobacteria (PGPR) are an important bacterial resource as biofertilizers, which can promote plant growth and increase crop yields. In this study, isolation, characterization, and its effect on plant promotion were assessed by isolating PGPR using soil samples collected from Jimma, Ethiopia. Out of 10 isolates, three of them (JEC3, JEC4, and JEC7) exhibited PSB traits in Pikovskaya media by solubilizing phosphate and producing IAA. Of the three isolates, JEC4 recorded the maximum phosphate solubilization index (4.98), soluble phosphate (283 μg/ml), and IAA production (10.21 μg/ml). The findings of the isolates' morphological and biochemical tests showed that JEC3, JEC4, and JEC7 as Bacillus sp., Pseudomonas sp., and Enterobacter sp., respectively. The optimization of phosphate solubilization was done using different incubation times and sources of carbon and nitrogen. Furthermore, the isolate (JEC4) was tested for plant growth-promoting (PGP) activities and indicated the production of ammonia, hydrogen cyanide, and siderophore. JEC4 isolate showed a significant increase in sideways (%) seed germination, root and shoot length, fresh and dry weight of root, and shoot of maize in field experiments than the untreated ones. The results indicate that the JEC4 isolate can be developed as a biological fertilizer for promoting crop productivity and ecosystem dynamics.http://dx.doi.org/10.1155/2022/5115875 |
| spellingShingle | Yasin Ahmed Waday Ermias Girma Aklilu Mohammed Seid Bultum Venkata Ramayya Ancha Dejene Beyene Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth Applied and Environmental Soil Science |
| title | Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth |
| title_full | Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth |
| title_fullStr | Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth |
| title_full_unstemmed | Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth |
| title_short | Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Coffee Plantation Soils and Its Influence on Maize Growth |
| title_sort | isolation and characterization of plant growth promoting rhizobacteria from coffee plantation soils and its influence on maize growth |
| url | http://dx.doi.org/10.1155/2022/5115875 |
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