Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae
A 30-day feeding trial was conducted to determine the effects of dietary glycyrrhizin (GL) on survival, growth performance, expression of feeding-related genes, activities of digestive enzymes, antioxidant capacity, and expression of inflammatory factors of large yellow croaker larvae with an initia...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2022/5508120 |
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| author | Yuntao Wang Wenxuan Xu Jianmin Zhang Jiahui Liu Zhen Wang Yongtao Liu Kangsen Mai Qinghui Ai |
| author_facet | Yuntao Wang Wenxuan Xu Jianmin Zhang Jiahui Liu Zhen Wang Yongtao Liu Kangsen Mai Qinghui Ai |
| author_sort | Yuntao Wang |
| collection | DOAJ |
| description | A 30-day feeding trial was conducted to determine the effects of dietary glycyrrhizin (GL) on survival, growth performance, expression of feeding-related genes, activities of digestive enzymes, antioxidant capacity, and expression of inflammatory factors of large yellow croaker larvae with an initial weight of 3.78±0.27 mg. Four 53.80% crude protein and 16.40% crude lipid diets were formulated with supplementation of 0%, 0.005%, 0.01%, and 0.02% GL, respectively. Results indicated that larvae fed diets with GL had higher survival rate and specific growth rate than the control (P<0.05). Compared with the control, the mRNA expression of orexigenic factor genes including neuropeptide Y (npy) and agouti-related protein (agrp) were significantly increased in larvae fed the diet with 0.005% GL, while the mRNA expression of anorexigenic factor genes including thyrotropin-releasing hormone (trh), cocaine and amphetamine regulated transcript (cart), and leptin receptor (lepr) were significantly decreased in larvae fed the diet with 0.005% GL (P<0.05). The trypsin activity in larvae fed the diet with 0.005% GL was significantly higher than the control (P<0.05). The alkaline phosphatase (AKP) activity in larvae fed the diet with 0.01% GL was significantly higher than the control (P<0.05). A clear increase of total glutathione (T-GSH) content, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) was observed in larvae fed the diet with 0.01% GL compared with the control (P<0.05). Moreover, the mRNA expression of interleukin-1β (il-1β) and interleukin-6 (il-6) (proinflammatory genes) in larvae fed the diet with 0.02% GL were significantly lower than the control (P<0.05). In conclusion, the supplementation of 0.005% -0.01% GL could enhance the expression of orexigenic factor genes, activities of digestive enzymes and antioxidant capacity, ultimately improving the survival, and growth performance of large yellow croaker larvae. |
| format | Article |
| id | doaj-art-9ba64b4f8a624bfa890b6c2fcead304e |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-9ba64b4f8a624bfa890b6c2fcead304e2025-02-03T06:05:01ZengWileyAquaculture Nutrition1365-20952022-01-01202210.1155/2022/5508120Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) LarvaeYuntao Wang0Wenxuan Xu1Jianmin Zhang2Jiahui Liu3Zhen Wang4Yongtao Liu5Kangsen Mai6Qinghui Ai7Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs)A 30-day feeding trial was conducted to determine the effects of dietary glycyrrhizin (GL) on survival, growth performance, expression of feeding-related genes, activities of digestive enzymes, antioxidant capacity, and expression of inflammatory factors of large yellow croaker larvae with an initial weight of 3.78±0.27 mg. Four 53.80% crude protein and 16.40% crude lipid diets were formulated with supplementation of 0%, 0.005%, 0.01%, and 0.02% GL, respectively. Results indicated that larvae fed diets with GL had higher survival rate and specific growth rate than the control (P<0.05). Compared with the control, the mRNA expression of orexigenic factor genes including neuropeptide Y (npy) and agouti-related protein (agrp) were significantly increased in larvae fed the diet with 0.005% GL, while the mRNA expression of anorexigenic factor genes including thyrotropin-releasing hormone (trh), cocaine and amphetamine regulated transcript (cart), and leptin receptor (lepr) were significantly decreased in larvae fed the diet with 0.005% GL (P<0.05). The trypsin activity in larvae fed the diet with 0.005% GL was significantly higher than the control (P<0.05). The alkaline phosphatase (AKP) activity in larvae fed the diet with 0.01% GL was significantly higher than the control (P<0.05). A clear increase of total glutathione (T-GSH) content, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) was observed in larvae fed the diet with 0.01% GL compared with the control (P<0.05). Moreover, the mRNA expression of interleukin-1β (il-1β) and interleukin-6 (il-6) (proinflammatory genes) in larvae fed the diet with 0.02% GL were significantly lower than the control (P<0.05). In conclusion, the supplementation of 0.005% -0.01% GL could enhance the expression of orexigenic factor genes, activities of digestive enzymes and antioxidant capacity, ultimately improving the survival, and growth performance of large yellow croaker larvae.http://dx.doi.org/10.1155/2022/5508120 |
| spellingShingle | Yuntao Wang Wenxuan Xu Jianmin Zhang Jiahui Liu Zhen Wang Yongtao Liu Kangsen Mai Qinghui Ai Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae Aquaculture Nutrition |
| title | Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae |
| title_full | Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae |
| title_fullStr | Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae |
| title_full_unstemmed | Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae |
| title_short | Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae |
| title_sort | effects of glycyrrhizin gl supplementation on survival growth performance expression of feeding related genes activities of digestive enzymes antioxidant capacity and expression of inflammatory factors in large yellow croaker larimichthys crocea larvae |
| url | http://dx.doi.org/10.1155/2022/5508120 |
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