T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease
Background: Chronic kidney disease (CKD) patients often experience dysregulated inflammation, particularly when compounded by comorbidities such as type 2 diabetes (T2D). Objective: The aim of this study was to determine whether T2D influences the profile of memory T lymphocytes, regulatory T cells...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2227-9059/13/1/3 |
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| author | Ana Cecilia Granda Alacote Gabriela Goyoneche Linares María Gracia Castañeda Torrico Daysi Zulema Diaz-Obregón Michael Bryant Castro Núñez Alexis Germán Murillo Carrasco Cesar Liendo Liendo Katherine Susan Rufasto Goche Víctor Arrunátegui Correa Joel de León Delgado |
| author_facet | Ana Cecilia Granda Alacote Gabriela Goyoneche Linares María Gracia Castañeda Torrico Daysi Zulema Diaz-Obregón Michael Bryant Castro Núñez Alexis Germán Murillo Carrasco Cesar Liendo Liendo Katherine Susan Rufasto Goche Víctor Arrunátegui Correa Joel de León Delgado |
| author_sort | Ana Cecilia Granda Alacote |
| collection | DOAJ |
| description | Background: Chronic kidney disease (CKD) patients often experience dysregulated inflammation, particularly when compounded by comorbidities such as type 2 diabetes (T2D). Objective: The aim of this study was to determine whether T2D influences the profile of memory T lymphocytes, regulatory T cells (Tregs), and the gene expression of transcription factors such as <i>T-bet (Tbx21)</i>, <i>GATA3</i>, <i>RORyT (RORC)</i>, and <i>FOXP3</i> in CKD patients. Methods: Twenty-two CKD patients undergoing hemodialysis were selected for the study. Flow cytometry was used to identify naïve T cells, Tregs (CD4+CD25+CD127-), central memory T lymphocytes (CCR7+CD45RA-), effector memory T lymphocytes (CCR7-CD45RA-), and TEMRA cells (CCR7-CD45RA+). The expression of helper T cell differentiation regulatory genes was assessed using real-time RT-PCR. Results: Both helper and cytotoxic effector memory T cell populations were found to be higher than naïve lymphocytes in CKD patients, regardless of T2D status. However, Tregs were significantly more frequent in diabetic CKD patients (5.1 ± 2.6%) compared to non-diabetic patients (2.8 ± 3.1%). In terms of transcription factor expression, a significant correlation was observed between T-bet and <i>FOXP3</i> in diabetic patients, and between RORyT and FOXP3 in non-diabetic patients. Conclusions: While T2D does not notably alter the distribution of memory T cells in CKD patients, it significantly impacts the frequency of Tregs and their correlation with pro-inflammatory transcription factors like <i>T-bet (Tbx21)</i> and <i>RORyT</i>. |
| format | Article |
| id | doaj-art-9ba17252595d4a218263948403e1aabd |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Biomedicines |
| spelling | doaj-art-9ba17252595d4a218263948403e1aabd2025-01-24T13:23:41ZengMDPI AGBiomedicines2227-90592024-12-01131310.3390/biomedicines13010003T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney DiseaseAna Cecilia Granda Alacote0Gabriela Goyoneche Linares1María Gracia Castañeda Torrico2Daysi Zulema Diaz-Obregón3Michael Bryant Castro Núñez4Alexis Germán Murillo Carrasco5Cesar Liendo Liendo6Katherine Susan Rufasto Goche7Víctor Arrunátegui Correa8Joel de León Delgado9Faculty of Natural Sciences and Mathematics, Universidad Nacional Federico Villarreal, Lima 15001, PeruFaculty of Natural Sciences and Mathematics, Universidad Nacional Federico Villarreal, Lima 15001, PeruONG Innovation and Science for the Care and Support of Society–INNOVACARE, Lima 15036, PeruHealth Technology Assessment and Research Institute-EsSalud, Lima 15072, PeruFaculty of Medicine, Universidad Nacional Federico Villarreal, Lima 15001, PeruImmunology and Cancer Research Group-IMMUCA, Lima 15001, PeruNephrology Center-CENESA, Lima 15001, PeruPostgraduate School, Universidad Nacional Federico Villarreal, Lima 15001, PeruFaculty of Human Medicine, University of San Martín de Porres, Lima 15011, PeruCenter of Virology Research, Faculty of Human Medicine, University of San Martín de Porres, Lima 15011, PeruBackground: Chronic kidney disease (CKD) patients often experience dysregulated inflammation, particularly when compounded by comorbidities such as type 2 diabetes (T2D). Objective: The aim of this study was to determine whether T2D influences the profile of memory T lymphocytes, regulatory T cells (Tregs), and the gene expression of transcription factors such as <i>T-bet (Tbx21)</i>, <i>GATA3</i>, <i>RORyT (RORC)</i>, and <i>FOXP3</i> in CKD patients. Methods: Twenty-two CKD patients undergoing hemodialysis were selected for the study. Flow cytometry was used to identify naïve T cells, Tregs (CD4+CD25+CD127-), central memory T lymphocytes (CCR7+CD45RA-), effector memory T lymphocytes (CCR7-CD45RA-), and TEMRA cells (CCR7-CD45RA+). The expression of helper T cell differentiation regulatory genes was assessed using real-time RT-PCR. Results: Both helper and cytotoxic effector memory T cell populations were found to be higher than naïve lymphocytes in CKD patients, regardless of T2D status. However, Tregs were significantly more frequent in diabetic CKD patients (5.1 ± 2.6%) compared to non-diabetic patients (2.8 ± 3.1%). In terms of transcription factor expression, a significant correlation was observed between T-bet and <i>FOXP3</i> in diabetic patients, and between RORyT and FOXP3 in non-diabetic patients. Conclusions: While T2D does not notably alter the distribution of memory T cells in CKD patients, it significantly impacts the frequency of Tregs and their correlation with pro-inflammatory transcription factors like <i>T-bet (Tbx21)</i> and <i>RORyT</i>.https://www.mdpi.com/2227-9059/13/1/3chronic kidney diseasetype 2 diabetestranscription factorsT cells |
| spellingShingle | Ana Cecilia Granda Alacote Gabriela Goyoneche Linares María Gracia Castañeda Torrico Daysi Zulema Diaz-Obregón Michael Bryant Castro Núñez Alexis Germán Murillo Carrasco Cesar Liendo Liendo Katherine Susan Rufasto Goche Víctor Arrunátegui Correa Joel de León Delgado T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease Biomedicines chronic kidney disease type 2 diabetes transcription factors T cells |
| title | T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease |
| title_full | T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease |
| title_fullStr | T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease |
| title_full_unstemmed | T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease |
| title_short | T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease |
| title_sort | t cell subpopulations and differentiation bias in diabetic and non diabetic patients with chronic kidney disease |
| topic | chronic kidney disease type 2 diabetes transcription factors T cells |
| url | https://www.mdpi.com/2227-9059/13/1/3 |
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