Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation
ABSTRACT Limnospira can colonize a wide variety of environments (e.g., freshwater, brackish, alkaline, or alkaline-saline water) and develop dominant and even permanent blooms that overshadow and limit the diversity of adjacent phototrophs, especially in alkaline and saline environments. Previous ph...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01901-24 |
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| author | Théotime Roussel Cédric Hubas Sébastien Halary Mathias Chynel Charlotte Duval Jean-Paul Cadoret Tarik Meziane Léa Vernès Claude Yéprémian Cécile Bernard Benjamin Marie |
| author_facet | Théotime Roussel Cédric Hubas Sébastien Halary Mathias Chynel Charlotte Duval Jean-Paul Cadoret Tarik Meziane Léa Vernès Claude Yéprémian Cécile Bernard Benjamin Marie |
| author_sort | Théotime Roussel |
| collection | DOAJ |
| description | ABSTRACT Limnospira can colonize a wide variety of environments (e.g., freshwater, brackish, alkaline, or alkaline-saline water) and develop dominant and even permanent blooms that overshadow and limit the diversity of adjacent phototrophs, especially in alkaline and saline environments. Previous phylogenomic analysis of Limnospira allowed us to distinguish two major phylogenetic clades (I and II) but failed to clearly segregate strains according to their respective habitats in terms of salinity or biogeography. In the present work, we attempted to determine whether Limnospira displays metabolic signatures specific to its different habitats, particularly brackish and alkaline-saline ecosystems. The impact of accessory gene repertoires on respective chemical adaptations was also determined. In complement of our previous phylogenomic investigation of Limnospira (Roussel et al., 2023), we develop a specific analysis of the metabolomic diversity of 93 strains of Limnospira, grown under standardized lab culture conditions. Overall, this original work showed distinct chemical fingerprints that were correlated with the respective biogeographic origins of the strains. The molecules that most distinguished the different Limnospira geographic groups were sugars, lipids, peptides, photosynthetic pigments, and antioxidants. Interestingly, these molecular enrichments might represent consequent adaptations to conditions of salinity, light, and oxidative stress in their respective sampling environments. Although the genes specifically involved in the production of these components remain unknown, we hypothesized that within extreme environments, such as those colonized by Limnospira, a large set of flexible genes could support the production of peculiar metabolite sets providing remarkable adaptations to specific local environmental conditions.IMPORTANCELimnospira are ubiquitous cyanobacteria with remarkable adaptive strategies allowing them to colonize and dominate a wide range of alkaline-saline environments worldwide. Phylogenomic analysis of Limnospira revealed two distinct major phylogenetic clades but failed to clearly segregate strains according to their habitats in terms of salinity or biogeography. We hypothesized that the genes found within this variable portion of the genome of these clades could be involved in the adaptation of Limnospira to local environmental conditions. In the present paper, we attempted to determine whether Limnospira displayed metabolic signatures specific to its different habitats. We also sought to understand the impact of the accessory gene repertoire on respective chemical adaptations. |
| format | Article |
| id | doaj-art-9b9b83c2536b43e097af11c92fe0e5c8 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
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| series | Microbiology Spectrum |
| spelling | doaj-art-9b9b83c2536b43e097af11c92fe0e5c82025-02-04T14:03:41ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.01901-24Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptationThéotime Roussel0Cédric Hubas1Sébastien Halary2Mathias Chynel3Charlotte Duval4Jean-Paul Cadoret5Tarik Meziane6Léa Vernès7Claude Yéprémian8Cécile Bernard9Benjamin Marie10UMR7245 MCAM MNHN-CNRS, Muséum National d’Histoire Naturelle, Paris, FranceUMR 7208 BOREA MNHN-CNRS-SU-IRD, Muséum d’Histoire Naturelle, Paris, FranceUMR7245 MCAM MNHN-CNRS, Muséum National d’Histoire Naturelle, Paris, FranceUMR 7208 BOREA MNHN-CNRS-SU-IRD, Muséum d’Histoire Naturelle, Paris, FranceUMR7245 MCAM MNHN-CNRS, Muséum National d’Histoire Naturelle, Paris, FranceAlgama, Paris, FranceUMR 7208 BOREA MNHN-CNRS-SU-IRD, Muséum d’Histoire Naturelle, Paris, FranceAlgama, Paris, FranceUMR7245 MCAM MNHN-CNRS, Muséum National d’Histoire Naturelle, Paris, FranceUMR7245 MCAM MNHN-CNRS, Muséum National d’Histoire Naturelle, Paris, FranceUMR7245 MCAM MNHN-CNRS, Muséum National d’Histoire Naturelle, Paris, FranceABSTRACT Limnospira can colonize a wide variety of environments (e.g., freshwater, brackish, alkaline, or alkaline-saline water) and develop dominant and even permanent blooms that overshadow and limit the diversity of adjacent phototrophs, especially in alkaline and saline environments. Previous phylogenomic analysis of Limnospira allowed us to distinguish two major phylogenetic clades (I and II) but failed to clearly segregate strains according to their respective habitats in terms of salinity or biogeography. In the present work, we attempted to determine whether Limnospira displays metabolic signatures specific to its different habitats, particularly brackish and alkaline-saline ecosystems. The impact of accessory gene repertoires on respective chemical adaptations was also determined. In complement of our previous phylogenomic investigation of Limnospira (Roussel et al., 2023), we develop a specific analysis of the metabolomic diversity of 93 strains of Limnospira, grown under standardized lab culture conditions. Overall, this original work showed distinct chemical fingerprints that were correlated with the respective biogeographic origins of the strains. The molecules that most distinguished the different Limnospira geographic groups were sugars, lipids, peptides, photosynthetic pigments, and antioxidants. Interestingly, these molecular enrichments might represent consequent adaptations to conditions of salinity, light, and oxidative stress in their respective sampling environments. Although the genes specifically involved in the production of these components remain unknown, we hypothesized that within extreme environments, such as those colonized by Limnospira, a large set of flexible genes could support the production of peculiar metabolite sets providing remarkable adaptations to specific local environmental conditions.IMPORTANCELimnospira are ubiquitous cyanobacteria with remarkable adaptive strategies allowing them to colonize and dominate a wide range of alkaline-saline environments worldwide. Phylogenomic analysis of Limnospira revealed two distinct major phylogenetic clades but failed to clearly segregate strains according to their habitats in terms of salinity or biogeography. We hypothesized that the genes found within this variable portion of the genome of these clades could be involved in the adaptation of Limnospira to local environmental conditions. In the present paper, we attempted to determine whether Limnospira displayed metabolic signatures specific to its different habitats. We also sought to understand the impact of the accessory gene repertoire on respective chemical adaptations.https://journals.asm.org/doi/10.1128/spectrum.01901-24cyanobacteriametabolomicsgenomicsadaptationmicro-diversity |
| spellingShingle | Théotime Roussel Cédric Hubas Sébastien Halary Mathias Chynel Charlotte Duval Jean-Paul Cadoret Tarik Meziane Léa Vernès Claude Yéprémian Cécile Bernard Benjamin Marie Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation Microbiology Spectrum cyanobacteria metabolomics genomics adaptation micro-diversity |
| title | Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation |
| title_full | Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation |
| title_fullStr | Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation |
| title_full_unstemmed | Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation |
| title_short | Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation |
| title_sort | limnospira cyanobacteria chemical fingerprint reveals local molecular adaptation |
| topic | cyanobacteria metabolomics genomics adaptation micro-diversity |
| url | https://journals.asm.org/doi/10.1128/spectrum.01901-24 |
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