Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire
This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multi...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/9532170 |
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| author | Ollo Kambire Ama Antoinette Adingra Konan Mathurin Yao Rose Koffi-Nevry |
| author_facet | Ollo Kambire Ama Antoinette Adingra Konan Mathurin Yao Rose Koffi-Nevry |
| author_sort | Ollo Kambire |
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| description | This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 19 from sediment, and 1 from crab) were positive for virulence genes, including 34 positive for elt (ETEC), 46 positive for est (ETEC), 24 positive for both elt and est, 6 positive for stx (EHEC), 1 positive for both stx + est, and 1 positive for both stx + elt. Genes eae (EPEC) and AggR (EAEC) were not detected. Nine serogroups (O114, O127, O55, O111, O86, O119, O126, O128, and O142) were identified. This study revealed the presence of diarrheagenic and nondiarrheagenic E. coli and potential public health risks if fishery products are not appropriately cooked. |
| format | Article |
| id | doaj-art-9b7ce8cf08eb46da8848ebf774ccfeb6 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-9b7ce8cf08eb46da8848ebf774ccfeb62025-02-03T06:43:53ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/95321709532170Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’IvoireOllo Kambire0Ama Antoinette Adingra1Konan Mathurin Yao2Rose Koffi-Nevry3Department of Biochemistry and Food Sciences, University of Peleforo Gon Coulibaly, BP 1328, Korhogo, Côte d’IvoireLaboratory of Microbiology, Oceanographic Research Center, BP V 18, Abidjan, Côte d’IvoireDepartment of Biochemistry and Food Sciences, University of Peleforo Gon Coulibaly, BP 1328, Korhogo, Côte d’IvoireLaboratory of Biotechnology and Food Microbiology (UFR/STA), University of Nangui Abrogoua, 02 BP 801 Abidjan, Côte d’IvoireThis study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 19 from sediment, and 1 from crab) were positive for virulence genes, including 34 positive for elt (ETEC), 46 positive for est (ETEC), 24 positive for both elt and est, 6 positive for stx (EHEC), 1 positive for both stx + est, and 1 positive for both stx + elt. Genes eae (EPEC) and AggR (EAEC) were not detected. Nine serogroups (O114, O127, O55, O111, O86, O119, O126, O128, and O142) were identified. This study revealed the presence of diarrheagenic and nondiarrheagenic E. coli and potential public health risks if fishery products are not appropriately cooked.http://dx.doi.org/10.1155/2017/9532170 |
| spellingShingle | Ollo Kambire Ama Antoinette Adingra Konan Mathurin Yao Rose Koffi-Nevry Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire International Journal of Microbiology |
| title | Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire |
| title_full | Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire |
| title_fullStr | Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire |
| title_full_unstemmed | Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire |
| title_short | Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire |
| title_sort | prevalence of virulence genes associated with diarrheagenic pathotypes of escherichia coli isolates from water sediment fish and crab in aby lagoon cote d ivoire |
| url | http://dx.doi.org/10.1155/2017/9532170 |
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