Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation

The impregnation of the polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with mango leaves extract (MLE) was carried out using the Supercritical Solvent Impregnation method. The impregnation loads, antioxidant compounds and physical properties of impregnated polymers were investigated w...

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Main Authors: Ludisbel León-Marcos, Antonio Montes, Diego Valor, Ignacio García-Casas, Noelia D. Machado, Clara Pereyra
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Journal of CO2 Utilization
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Online Access:http://www.sciencedirect.com/science/article/pii/S2212982025000460
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author Ludisbel León-Marcos
Antonio Montes
Diego Valor
Ignacio García-Casas
Noelia D. Machado
Clara Pereyra
author_facet Ludisbel León-Marcos
Antonio Montes
Diego Valor
Ignacio García-Casas
Noelia D. Machado
Clara Pereyra
author_sort Ludisbel León-Marcos
collection DOAJ
description The impregnation of the polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with mango leaves extract (MLE) was carried out using the Supercritical Solvent Impregnation method. The impregnation loads, antioxidant compounds and physical properties of impregnated polymers were investigated with the variation of operating parameters (temperature (35 and 55 °C), pressure (10, 20 and 30 MPa) and depressurization rate (0.1 and 5 MPa/min)). Furthermore, the influence of varying impregnation times (1, 3, 6, 10, 24 h) with 30 MPa, 35 °C and 5 MPa/min was also investigated and the antimicrobial capacity (AC) was determined. The results showed a decrease of the impregnation loads, antioxidant and antimicrobial capacities with increasing impregnation time. At 30 MPa, 35 ºC and 5 MPa/min with an impregnation time of 1 h, impregnation loads and antioxidant capacities of 2.82 ± 0.04 mg MLE/100 mg PHB-HV and 2.77 ± 0.10 mg AC/100 mg PHB-HV, respectively, and a growth inhibition percentage against Staphylococcus aureus and Escherichia coli bacteria greater than 60 % were obtained.
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spelling doaj-art-9b52de33e6564508a192520cda1b284b2025-08-20T02:29:35ZengElsevierJournal of CO2 Utilization2212-98392025-05-019510306210.1016/j.jcou.2025.103062Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnationLudisbel León-Marcos0Antonio Montes1Diego Valor2Ignacio García-Casas3Noelia D. Machado4Clara Pereyra5Corresponding author.; Department of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, International Excellence Agrifood Campus (CeiA3), Wine and Agrifood Research Institute (IVAGRO), 11510 Puerto Real, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, International Excellence Agrifood Campus (CeiA3), Wine and Agrifood Research Institute (IVAGRO), 11510 Puerto Real, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, International Excellence Agrifood Campus (CeiA3), Wine and Agrifood Research Institute (IVAGRO), 11510 Puerto Real, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, International Excellence Agrifood Campus (CeiA3), Wine and Agrifood Research Institute (IVAGRO), 11510 Puerto Real, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, International Excellence Agrifood Campus (CeiA3), Wine and Agrifood Research Institute (IVAGRO), 11510 Puerto Real, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, International Excellence Agrifood Campus (CeiA3), Wine and Agrifood Research Institute (IVAGRO), 11510 Puerto Real, Cadiz, SpainThe impregnation of the polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with mango leaves extract (MLE) was carried out using the Supercritical Solvent Impregnation method. The impregnation loads, antioxidant compounds and physical properties of impregnated polymers were investigated with the variation of operating parameters (temperature (35 and 55 °C), pressure (10, 20 and 30 MPa) and depressurization rate (0.1 and 5 MPa/min)). Furthermore, the influence of varying impregnation times (1, 3, 6, 10, 24 h) with 30 MPa, 35 °C and 5 MPa/min was also investigated and the antimicrobial capacity (AC) was determined. The results showed a decrease of the impregnation loads, antioxidant and antimicrobial capacities with increasing impregnation time. At 30 MPa, 35 ºC and 5 MPa/min with an impregnation time of 1 h, impregnation loads and antioxidant capacities of 2.82 ± 0.04 mg MLE/100 mg PHB-HV and 2.77 ± 0.10 mg AC/100 mg PHB-HV, respectively, and a growth inhibition percentage against Staphylococcus aureus and Escherichia coli bacteria greater than 60 % were obtained.http://www.sciencedirect.com/science/article/pii/S2212982025000460Poly(3-hydroxi-butyrate-co-3-hydroxy-valerate)Supercritical Solvent ImpregnationMangifera indica L. leavesActive food packaging
spellingShingle Ludisbel León-Marcos
Antonio Montes
Diego Valor
Ignacio García-Casas
Noelia D. Machado
Clara Pereyra
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation
Journal of CO2 Utilization
Poly(3-hydroxi-butyrate-co-3-hydroxy-valerate)
Supercritical Solvent Impregnation
Mangifera indica L. leaves
Active food packaging
title Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation
title_full Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation
title_fullStr Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation
title_full_unstemmed Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation
title_short Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) impregnated with mango leaves extracts using supercritical solvent impregnation
title_sort poly 3 hydroxybutyrate co 3 hydroxyvalerate phb hv impregnated with mango leaves extracts using supercritical solvent impregnation
topic Poly(3-hydroxi-butyrate-co-3-hydroxy-valerate)
Supercritical Solvent Impregnation
Mangifera indica L. leaves
Active food packaging
url http://www.sciencedirect.com/science/article/pii/S2212982025000460
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