Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population
Pandemic SARS-CoV-2 has caused unprecedented mortalities. Vaccine is in urgent need to stop the pandemic. Despite great progresses on SARS-CoV-2 vaccine development, the efficacy of the vaccines remains to be determined. Deciphering the interactions of the viral epitopes with the elicited neutralizi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1815591 |
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| author | Zhigang Yi Yun Ling Xiaonan Zhang Jieliang Chen Kongying Hu Yuyan Wang Wuhui Song Tianlei Ying Rong Zhang HongZhou Lu Zhenghong Yuan |
| author_facet | Zhigang Yi Yun Ling Xiaonan Zhang Jieliang Chen Kongying Hu Yuyan Wang Wuhui Song Tianlei Ying Rong Zhang HongZhou Lu Zhenghong Yuan |
| author_sort | Zhigang Yi |
| collection | DOAJ |
| description | Pandemic SARS-CoV-2 has caused unprecedented mortalities. Vaccine is in urgent need to stop the pandemic. Despite great progresses on SARS-CoV-2 vaccine development, the efficacy of the vaccines remains to be determined. Deciphering the interactions of the viral epitopes with the elicited neutralizing antibodies in convalescent population inspires the vaccine development. In this study, we devised a peptide array composed of 20-mer overlapped peptides of spike (S), membrane (M) and envelope (E) proteins, and performed a screening with 120 COVID-19 convalescent sera and 24 non-COVID-19 sera. We identified five SARS-CoV-2-specific dominant epitopes that reacted with above 40% COVID-19 convalescent sera. Of note, two peptides non-specifically interacted with most of the non-COVID-19 sera. Neutralization assay indicated that only five sera completely blocked viral infection at the dilution of 1:200. By using a peptide-compete neutralizing assay, we found that three dominant epitopes partially competed the neutralization activity of several convalescent sera, suggesting antibodies elicited by these epitopes played an important role in neutralizing viral infection. The epitopes we identified in this study may serve as vaccine candidates to elicit neutralizing antibodies in most vaccinated people or specific antigens for SARS-CoV-2 diagnosis. |
| format | Article |
| id | doaj-art-9b3e09d74dfb48fd913d3d9f822f709c |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-9b3e09d74dfb48fd913d3d9f822f709c2025-08-20T02:11:34ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911988199610.1080/22221751.2020.1815591Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent populationZhigang Yi0Yun Ling1Xiaonan Zhang2Jieliang Chen3Kongying Hu4Yuyan Wang5Wuhui Song6Tianlei Ying7Rong Zhang8HongZhou Lu9Zhenghong Yuan10Shanghai Public Health Clinical Center, Fudan University, Shanghai, People’s Republic of ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, People’s Republic of ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, People’s Republic of ChinaKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaPandemic SARS-CoV-2 has caused unprecedented mortalities. Vaccine is in urgent need to stop the pandemic. Despite great progresses on SARS-CoV-2 vaccine development, the efficacy of the vaccines remains to be determined. Deciphering the interactions of the viral epitopes with the elicited neutralizing antibodies in convalescent population inspires the vaccine development. In this study, we devised a peptide array composed of 20-mer overlapped peptides of spike (S), membrane (M) and envelope (E) proteins, and performed a screening with 120 COVID-19 convalescent sera and 24 non-COVID-19 sera. We identified five SARS-CoV-2-specific dominant epitopes that reacted with above 40% COVID-19 convalescent sera. Of note, two peptides non-specifically interacted with most of the non-COVID-19 sera. Neutralization assay indicated that only five sera completely blocked viral infection at the dilution of 1:200. By using a peptide-compete neutralizing assay, we found that three dominant epitopes partially competed the neutralization activity of several convalescent sera, suggesting antibodies elicited by these epitopes played an important role in neutralizing viral infection. The epitopes we identified in this study may serve as vaccine candidates to elicit neutralizing antibodies in most vaccinated people or specific antigens for SARS-CoV-2 diagnosis.https://www.tandfonline.com/doi/10.1080/22221751.2020.1815591SARS-CoV-2COVID-19epitopeneutralizing antibodyvaccinediagnosis |
| spellingShingle | Zhigang Yi Yun Ling Xiaonan Zhang Jieliang Chen Kongying Hu Yuyan Wang Wuhui Song Tianlei Ying Rong Zhang HongZhou Lu Zhenghong Yuan Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population Emerging Microbes and Infections SARS-CoV-2 COVID-19 epitope neutralizing antibody vaccine diagnosis |
| title | Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population |
| title_full | Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population |
| title_fullStr | Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population |
| title_full_unstemmed | Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population |
| title_short | Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population |
| title_sort | functional mapping of b cell linear epitopes of sars cov 2 in covid 19 convalescent population |
| topic | SARS-CoV-2 COVID-19 epitope neutralizing antibody vaccine diagnosis |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1815591 |
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