CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1

CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1

ABSTRACT Lactiplantibacillus plantarum, a natural inhabitant of the human body, is a promising candidate vehicle for vaccine delivery. An obstacle in developing bacterial delivery vehicles is generating a production strain that lacks antibiotic resistance genes and contains minimal foreign DNA. To d...

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Bibliographic Details
Main Authors: Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen
Format: Article
Language:English
Published: American Society for Microbiology 2025-02-01
Series:Microbiology Spectrum
Subjects:
genome editing
gene insertion
Lactiplantibacillus plantarum
CRISPR
surface display
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02025-24
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