The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration
Objective(s): Platelet-rich plasma (PRP) contains multiple growth hormones that may stimulate tissue repair. We aimed to assess PRP’s efficacy and explore possible mechanisms using the intervertebral disc degeneration (IDD) model. Materials and Methods: A total of 48 male Sprague-Dawley (SD) rats we...
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Mashhad University of Medical Sciences
2025-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_25233_b6707a240a7962ae68e134e613620c50.pdf |
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| author | Jing Luan Qi Wang Wei Zheng Yongjin He |
| author_facet | Jing Luan Qi Wang Wei Zheng Yongjin He |
| author_sort | Jing Luan |
| collection | DOAJ |
| description | Objective(s): Platelet-rich plasma (PRP) contains multiple growth hormones that may stimulate tissue repair. We aimed to assess PRP’s efficacy and explore possible mechanisms using the intervertebral disc degeneration (IDD) model. Materials and Methods: A total of 48 male Sprague-Dawley (SD) rats were randomly divided into three groups: sham, IDD+PBS, and IDD+PRP (n=16, respectively). IL-1β (10 ng/ml) was used to establish a humanized IDD model in human lumbar nucleus pulposus (NP) tissues from 36 patients with degenerative disc disease. These NP cells were randomly divided into three groups: sham, IDD+PBS, and IDD+PRP (n=12, respectively). RT-PCR and western blot were used to detect the expression of aggrecan, collagen II, IL-1β, IL-6, TNF-α, Bcl-2, cleaved-Caspase 3, Bax and Akt/mTOR/p70S6K signaling pathway. A related assay kit was used to detect MDA, SOD, and GSH.Results: PRP affected the expression of aggrecan, collagen II, IL-1β, IL-6, TNF-α, MDA, SOD, GSH, Bcl-2, cleaved-Caspase 3, and Bax in IDD rats. Compared with the IDD+PBS group, the expression of p-mTOR, p-p70/S6K, and p-Akt was much lower in the rat IDD+PRP group (P<0.05). Similarly, with PRP treatment in the humanized IDD model, the expression of p-mTOR, p-p70/S6K, and p-Akt was also inhibited.Conclusion: PRP may be a potential therapy for IDD via the mTOR signaling pathway in regulating and affecting extracellular matrix degradation, inflammatory factors, oxidative stress, and apoptosis. |
| format | Article |
| id | doaj-art-9b0ba2523ac841cbbbed90e25b4564f5 |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-9b0ba2523ac841cbbbed90e25b4564f52025-08-20T02:48:06ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-03-0128339340010.22038/ijbms.2024.79218.1716325233The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degenerationJing Luan0Qi Wang1Wei Zheng2Yongjin He3Department of Pain, Tianjin First Central Hospital, Tianjin, 300110, ChinaDepartment of Anesthesiology, Tianjin First Central Hospital, Tianjin, 300110, ChinaDepartment of Pain, Tianjin First Central Hospital, Tianjin, 300110, ChinaDepartment of Pain, Tianjin First Central Hospital, Tianjin, 300110, ChinaObjective(s): Platelet-rich plasma (PRP) contains multiple growth hormones that may stimulate tissue repair. We aimed to assess PRP’s efficacy and explore possible mechanisms using the intervertebral disc degeneration (IDD) model. Materials and Methods: A total of 48 male Sprague-Dawley (SD) rats were randomly divided into three groups: sham, IDD+PBS, and IDD+PRP (n=16, respectively). IL-1β (10 ng/ml) was used to establish a humanized IDD model in human lumbar nucleus pulposus (NP) tissues from 36 patients with degenerative disc disease. These NP cells were randomly divided into three groups: sham, IDD+PBS, and IDD+PRP (n=12, respectively). RT-PCR and western blot were used to detect the expression of aggrecan, collagen II, IL-1β, IL-6, TNF-α, Bcl-2, cleaved-Caspase 3, Bax and Akt/mTOR/p70S6K signaling pathway. A related assay kit was used to detect MDA, SOD, and GSH.Results: PRP affected the expression of aggrecan, collagen II, IL-1β, IL-6, TNF-α, MDA, SOD, GSH, Bcl-2, cleaved-Caspase 3, and Bax in IDD rats. Compared with the IDD+PBS group, the expression of p-mTOR, p-p70/S6K, and p-Akt was much lower in the rat IDD+PRP group (P<0.05). Similarly, with PRP treatment in the humanized IDD model, the expression of p-mTOR, p-p70/S6K, and p-Akt was also inhibited.Conclusion: PRP may be a potential therapy for IDD via the mTOR signaling pathway in regulating and affecting extracellular matrix degradation, inflammatory factors, oxidative stress, and apoptosis.https://ijbms.mums.ac.ir/article_25233_b6707a240a7962ae68e134e613620c50.pdfakt/mtor/p70s6k-signaling pathwayapoptosisextracellular matrix- degradationinflammatory factors intervertebral disc degenerationoxidative stressplatelet-rich plasma |
| spellingShingle | Jing Luan Qi Wang Wei Zheng Yongjin He The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration Iranian Journal of Basic Medical Sciences akt/mtor/p70s6k-signaling pathway apoptosis extracellular matrix- degradation inflammatory factors intervertebral disc degeneration oxidative stress platelet-rich plasma |
| title | The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration |
| title_full | The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration |
| title_fullStr | The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration |
| title_full_unstemmed | The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration |
| title_short | The mTOR pathway is involved in the process of platelet-rich plasma improving intervertebral disc degeneration |
| title_sort | mtor pathway is involved in the process of platelet rich plasma improving intervertebral disc degeneration |
| topic | akt/mtor/p70s6k-signaling pathway apoptosis extracellular matrix- degradation inflammatory factors intervertebral disc degeneration oxidative stress platelet-rich plasma |
| url | https://ijbms.mums.ac.ir/article_25233_b6707a240a7962ae68e134e613620c50.pdf |
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