SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate
Transcription factors NANOG, OCT4, and SOX2 regulate self-renewal and pluripotency in human embryonic stem (hES) cells; however, their expression profiles during early differentiation of hES cells are unclear. In this study, we used multiparameter flow cytometric assay to detect all three transcript...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/298163 |
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| author | Ade Kallas Martin Pook Annika Trei Toivo Maimets |
| author_facet | Ade Kallas Martin Pook Annika Trei Toivo Maimets |
| author_sort | Ade Kallas |
| collection | DOAJ |
| description | Transcription factors NANOG, OCT4, and SOX2 regulate self-renewal and pluripotency in human embryonic stem (hES) cells; however, their expression profiles during early differentiation of hES cells are unclear. In this study, we used multiparameter flow cytometric assay to detect all three transcription factors (NANOG, OCT4, and SOX2) simultaneously at single cell level and monitored the changes in their expression during early differentiation towards endodermal lineage (induced by sodium butyrate). We observed at least four distinct populations of hES cells, characterized by specific expression patterns of NANOG, OCT4, and SOX2 and differentiation markers. Our results show that a single cell can express both differentiation and pluripotency markers at the same time, indicating a gradual mode of developmental transition in these cells. Notably, distinct regulation of SOX2 during early differentiation events was detected, highlighting the potential importance of this transcription factor for self-renewal of hES cells during differentiation. |
| format | Article |
| id | doaj-art-9ab50573cdac458b91086b7a178258d1 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9ab50573cdac458b91086b7a178258d12025-02-03T01:01:03ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/298163298163SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium ButyrateAde Kallas0Martin Pook1Annika Trei2Toivo Maimets3Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaInstitute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaInstitute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaInstitute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaTranscription factors NANOG, OCT4, and SOX2 regulate self-renewal and pluripotency in human embryonic stem (hES) cells; however, their expression profiles during early differentiation of hES cells are unclear. In this study, we used multiparameter flow cytometric assay to detect all three transcription factors (NANOG, OCT4, and SOX2) simultaneously at single cell level and monitored the changes in their expression during early differentiation towards endodermal lineage (induced by sodium butyrate). We observed at least four distinct populations of hES cells, characterized by specific expression patterns of NANOG, OCT4, and SOX2 and differentiation markers. Our results show that a single cell can express both differentiation and pluripotency markers at the same time, indicating a gradual mode of developmental transition in these cells. Notably, distinct regulation of SOX2 during early differentiation events was detected, highlighting the potential importance of this transcription factor for self-renewal of hES cells during differentiation.http://dx.doi.org/10.1155/2014/298163 |
| spellingShingle | Ade Kallas Martin Pook Annika Trei Toivo Maimets SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate Stem Cells International |
| title | SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate |
| title_full | SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate |
| title_fullStr | SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate |
| title_full_unstemmed | SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate |
| title_short | SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate |
| title_sort | sox2 is regulated differently from nanog and oct4 in human embryonic stem cells during early differentiation initiated with sodium butyrate |
| url | http://dx.doi.org/10.1155/2014/298163 |
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