Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients
Understanding the immune response generated by SARS-CoV-2 is critical for assessing efficient therapeutic protocols and gaining insights into the durability of protective immunity. The current work was aimed at studying the specific humoral responses against SARS-CoV-2 in Cuban COVID-19 convalescent...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/7112940 |
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| author | Ivette Orosa Vázquez Marianniz Díaz Yaima Zúñiga Rosales Klayris Amada Janoi Chang Ernesto Relova Hernández Yaima Tundidor Hilda Roblejo Balbuena Giselle Monzón Bárbara Torres Rives Enrique Noa Romero Danay Carrillo Valdés Irinia Valdivia Álvarez Aurora Delahanty Fernández Claudia Díaz Joaquín Solozabal Mileidys Gil Belinda Sánchez Gertrudis Rojas Beatriz Marcheco Tania Carmenate |
| author_facet | Ivette Orosa Vázquez Marianniz Díaz Yaima Zúñiga Rosales Klayris Amada Janoi Chang Ernesto Relova Hernández Yaima Tundidor Hilda Roblejo Balbuena Giselle Monzón Bárbara Torres Rives Enrique Noa Romero Danay Carrillo Valdés Irinia Valdivia Álvarez Aurora Delahanty Fernández Claudia Díaz Joaquín Solozabal Mileidys Gil Belinda Sánchez Gertrudis Rojas Beatriz Marcheco Tania Carmenate |
| author_sort | Ivette Orosa Vázquez |
| collection | DOAJ |
| description | Understanding the immune response generated by SARS-CoV-2 is critical for assessing efficient therapeutic protocols and gaining insights into the durability of protective immunity. The current work was aimed at studying the specific humoral responses against SARS-CoV-2 in Cuban COVID-19 convalescents. We developed suitable tools and methods based on ELISA methodology, for supporting this evaluation. Here, we describe the development of an ELISA for the quantification of anti-RBD IgG titers in a large number of samples and a similar test in the presence of NH4SCN as chaotropic agent for estimating the RBD specific antibody avidity. Additionally, a simple and rapid ELISA based on antibody-mediated blockage of the binding RBD-ACE2 was implemented for detecting, as a surrogate of conventional test, the levels of anti-RBD inhibitory antibodies in convalescent sera. In a cohort of 273 unvaccinated convalescents, we identified higher anti-RBD IgG titer (1 : 1,330, p <0.0001) and higher levels of inhibitory antibodies blocking RBD-ACE2 binding (1 : 216, p <0.05) among those who had recovered from severe illness. Our results suggest that disease severity, and not demographic features such as age, sex, and skin color, is the main determinant of the magnitude and neutralizing ability of the anti-RBD antibody response. An additional paired longitudinal assessment in 14 symptomatic convalescents revealed a decline in the antiviral antibody response and the persistence of neutralizing antibodies for at least 4 months after the onset of symptoms. Overall, SARS-CoV-2 infection elicits different levels of antibody response according to disease severity that declines over time and can be monitored using our homemade serological assays. |
| format | Article |
| id | doaj-art-9ab37f77e4cf4be4a5086f33ab50f49f |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9ab37f77e4cf4be4a5086f33ab50f49f2025-02-03T11:54:52ZengWileyJournal of Immunology Research2314-71562024-01-01202410.1155/2024/7112940Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered PatientsIvette Orosa Vázquez0Marianniz Díaz1Yaima Zúñiga Rosales2Klayris Amada3Janoi Chang4Ernesto Relova Hernández5Yaima Tundidor6Hilda Roblejo Balbuena7Giselle Monzón8Bárbara Torres Rives9Enrique Noa Romero10Danay Carrillo Valdés11Irinia Valdivia Álvarez12Aurora Delahanty Fernández13Claudia Díaz14Joaquín Solozabal15Mileidys Gil16Belinda Sánchez17Gertrudis Rojas18Beatriz Marcheco19Tania Carmenate20Center of Molecular ImmunologyCenter of Molecular ImmunologyNational Center of Medical GeneticsJulio Trigo HospitalCenter of Molecular ImmunologyCenter of Molecular ImmunologyCenter of Molecular ImmunologyNational Center of Medical GeneticsNational Center of Medical GeneticsNational Center of Medical GeneticsResearch Center of Civil DefenseResearch Center of Civil DefenseCentre for ImmunoassaysCentre for ImmunoassaysCenter of Molecular ImmunologyCenter of Molecular ImmunologyJulio Trigo HospitalCenter of Molecular ImmunologyCenter of Molecular ImmunologyNational Center of Medical GeneticsCenter of Molecular ImmunologyUnderstanding the immune response generated by SARS-CoV-2 is critical for assessing efficient therapeutic protocols and gaining insights into the durability of protective immunity. The current work was aimed at studying the specific humoral responses against SARS-CoV-2 in Cuban COVID-19 convalescents. We developed suitable tools and methods based on ELISA methodology, for supporting this evaluation. Here, we describe the development of an ELISA for the quantification of anti-RBD IgG titers in a large number of samples and a similar test in the presence of NH4SCN as chaotropic agent for estimating the RBD specific antibody avidity. Additionally, a simple and rapid ELISA based on antibody-mediated blockage of the binding RBD-ACE2 was implemented for detecting, as a surrogate of conventional test, the levels of anti-RBD inhibitory antibodies in convalescent sera. In a cohort of 273 unvaccinated convalescents, we identified higher anti-RBD IgG titer (1 : 1,330, p <0.0001) and higher levels of inhibitory antibodies blocking RBD-ACE2 binding (1 : 216, p <0.05) among those who had recovered from severe illness. Our results suggest that disease severity, and not demographic features such as age, sex, and skin color, is the main determinant of the magnitude and neutralizing ability of the anti-RBD antibody response. An additional paired longitudinal assessment in 14 symptomatic convalescents revealed a decline in the antiviral antibody response and the persistence of neutralizing antibodies for at least 4 months after the onset of symptoms. Overall, SARS-CoV-2 infection elicits different levels of antibody response according to disease severity that declines over time and can be monitored using our homemade serological assays.http://dx.doi.org/10.1155/2024/7112940 |
| spellingShingle | Ivette Orosa Vázquez Marianniz Díaz Yaima Zúñiga Rosales Klayris Amada Janoi Chang Ernesto Relova Hernández Yaima Tundidor Hilda Roblejo Balbuena Giselle Monzón Bárbara Torres Rives Enrique Noa Romero Danay Carrillo Valdés Irinia Valdivia Álvarez Aurora Delahanty Fernández Claudia Díaz Joaquín Solozabal Mileidys Gil Belinda Sánchez Gertrudis Rojas Beatriz Marcheco Tania Carmenate Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients Journal of Immunology Research |
| title | Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients |
| title_full | Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients |
| title_fullStr | Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients |
| title_full_unstemmed | Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients |
| title_short | Studying the Humoral Response against SARS-CoV-2 in Cuban COVID-19 Recovered Patients |
| title_sort | studying the humoral response against sars cov 2 in cuban covid 19 recovered patients |
| url | http://dx.doi.org/10.1155/2024/7112940 |
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