Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres
Ischemic stroke, resulting from the obstruction of blood flow to the brain, remains a leading cause of morbidity and mortality worldwide. Traditional imaging modalities, such as magnetic resonance imaging and computed tomography, while effective for identifying stroke locations, are often limited in...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000717 |
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| author | Ziyue Li Yu Qiang Dongli Chen Dehong Hu Duyang Gao Xiaohua Xu Lei Sun Yingjia Li Weibao Qiu Zonghai Sheng |
| author_facet | Ziyue Li Yu Qiang Dongli Chen Dehong Hu Duyang Gao Xiaohua Xu Lei Sun Yingjia Li Weibao Qiu Zonghai Sheng |
| author_sort | Ziyue Li |
| collection | DOAJ |
| description | Ischemic stroke, resulting from the obstruction of blood flow to the brain, remains a leading cause of morbidity and mortality worldwide. Traditional imaging modalities, such as magnetic resonance imaging and computed tomography, while effective for identifying stroke locations, are often limited in their ability to detect early pathological changes due to constraints in spatial resolution and sensitivity. This study introduces a novel dual-modal imaging approach that employs indocyanine green-doped porous poly (lactic-co-glycolic acid) (PLGA) microspheres (ICG-pPLGA MPs) for super-resolution ultrasound and near-infrared II (NIR-II) fluorescence imaging of ischemic stroke. The porous structure of ICG-pPLGA MPs enhances their stability, prolongs their circulation time, and improves ultrasound contrast compared to commercial lipid microbubbles. Additionally, the NIR-II fluorescence allows for high-resolution and noninvasive visualization of superficial vasculature. In a rat model of ischemic stroke, we demonstrate the capability of ICG-pPLGA MPs to achieve high-resolution imaging of cerebrovascular structures and functions, surpassing the imaging performance of standard diffusion-weighted imaging. Our findings underscore the potential of this dual-modal imaging technique using ICG-pPLGA MPs to accurately characterize microvascular changes during ischemic events, thus offering valuable insights for early diagnosis and therapeutic monitoring. |
| format | Article |
| id | doaj-art-9ab07ddf1c6f4f5889bc2b793cfdc2fa |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-9ab07ddf1c6f4f5889bc2b793cfdc2fa2025-01-26T05:04:44ZengElsevierMaterials Today Bio2590-00642025-04-0131101513Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheresZiyue Li0Yu Qiang1Dongli Chen2Dehong Hu3Duyang Gao4Xiaohua Xu5Lei Sun6Yingjia Li7Weibao Qiu8Zonghai Sheng9Department of Medicine Ultrasonics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China; Research Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; The Hong Kong Polytechnic University, Department of Biomedical Engineering, Hong Kong, 999077, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Division of Ultrasound, The University of Hong Kong-Shenzhen Hospital, No.1, Haiyuan Road, Futian District, Shenzhen, 518053, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, ChinaResearch Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, ChinaDivision of Ultrasound, The University of Hong Kong-Shenzhen Hospital, No.1, Haiyuan Road, Futian District, Shenzhen, 518053, ChinaThe Hong Kong Polytechnic University, Department of Biomedical Engineering, Hong Kong, 999077, ChinaDepartment of Medicine Ultrasonics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China; Corresponding author.Research Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, China; Corresponding author.Research Center for Advanced Detection Materials and Medical Imaging Devices, Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen, 518055, China; Corresponding author.Ischemic stroke, resulting from the obstruction of blood flow to the brain, remains a leading cause of morbidity and mortality worldwide. Traditional imaging modalities, such as magnetic resonance imaging and computed tomography, while effective for identifying stroke locations, are often limited in their ability to detect early pathological changes due to constraints in spatial resolution and sensitivity. This study introduces a novel dual-modal imaging approach that employs indocyanine green-doped porous poly (lactic-co-glycolic acid) (PLGA) microspheres (ICG-pPLGA MPs) for super-resolution ultrasound and near-infrared II (NIR-II) fluorescence imaging of ischemic stroke. The porous structure of ICG-pPLGA MPs enhances their stability, prolongs their circulation time, and improves ultrasound contrast compared to commercial lipid microbubbles. Additionally, the NIR-II fluorescence allows for high-resolution and noninvasive visualization of superficial vasculature. In a rat model of ischemic stroke, we demonstrate the capability of ICG-pPLGA MPs to achieve high-resolution imaging of cerebrovascular structures and functions, surpassing the imaging performance of standard diffusion-weighted imaging. Our findings underscore the potential of this dual-modal imaging technique using ICG-pPLGA MPs to accurately characterize microvascular changes during ischemic events, thus offering valuable insights for early diagnosis and therapeutic monitoring.http://www.sciencedirect.com/science/article/pii/S2590006425000717NIR-II fluorescenceSuper-resolution ultrasoundIndocyanine greenPorous microsphereIschemic stroke |
| spellingShingle | Ziyue Li Yu Qiang Dongli Chen Dehong Hu Duyang Gao Xiaohua Xu Lei Sun Yingjia Li Weibao Qiu Zonghai Sheng Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres Materials Today Bio NIR-II fluorescence Super-resolution ultrasound Indocyanine green Porous microsphere Ischemic stroke |
| title | Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres |
| title_full | Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres |
| title_fullStr | Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres |
| title_full_unstemmed | Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres |
| title_short | Dual-modal super-resolution ultrasound and NIR-II fluorescence imaging of ischemic stroke with ICG-doped porous PLGA microspheres |
| title_sort | dual modal super resolution ultrasound and nir ii fluorescence imaging of ischemic stroke with icg doped porous plga microspheres |
| topic | NIR-II fluorescence Super-resolution ultrasound Indocyanine green Porous microsphere Ischemic stroke |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000717 |
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